Phytochemical Screening, Acute Toxicity , Analgesic And Anti-Inflammatory Effects Of Apricot Seeds Ethanolic Extracts

A., Ramadan; Kamel, Gehan; Awad, Nagwa E.; Shokry, Aya A.

doi:10.21608/javs.2018.62723

Phytochemical Screening, Acute Toxicity , Analgesic And Anti-Inflammatory Effects Of Apricot Seeds Ethanolic Extracts

Document Type : Original Article

Authors

¹ Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Cairo, Egypt.

² Department of Pharmacognosy, Pharmaceutical and Drug Industrial Research Division, National Research Center, Cairo, Egypt.

³ Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Cairo, Egypt

10.21608/javs.2018.62723

Abstract

The aim of present study was to investigate the phytochemical screening, acute toxicity and some pharmacological activities of ethanolic extract 70 % and 99.9% of apricot seeds. The phytochemical screening was done for determination of total phenolic compounds by Folin–Ciocalteu method, total flavonoids by aluminum chloride colorimetric method and total carotenoids by colorimetric method. The acute toxicity was done for determination of LD₅₀ in mice by oral administration of upgraded doses of the extracts. The anti-inflammatory activity was done in vivo by formalin-induced paw edema in rats. The analgesic activity was carried out in mice by writhing test and hot plate method. Phytochemical screening revealed that the amount of total phenolic compounds was 179.4 and 191.2 µg gallic acid equivalent / g dry extract and the amount of total flavonoids was 226.18 and 509.34 µg rutin equivalent / g dry extract and the amount of total carotenoids was 0.145 and 0.156 mg/g dry extract for 70 % and 99.9% ethanolic extract, respectively. The acute toxicity revealed that both extracts had no toxic symptoms in rats and no mortalities appear by upgraded doses of 1 g to 10 g / kg b.wt. The 70% and 99.9% ethanolic extracts exhibited significant analgesic and anti-inflammatory activites in a dose of 100 mg/kg b.wt. of both extracts. These results suggest that apricot seed extracts contain significant level of safe non-toxic phytochemical substances that have significant analgesic and anti-inflammatory activities.

Keywords

Main Subjects

Pharmacology and toxicology

References

ABDEL-RAHMAN, M.K. 2011. Can apricot kernels fatty acids delay the atrophied hepatocytes from progression to fibrosis in dimethyl nitrosamine (DMN) -induced liver injury in rats ? Lipids in Health and Disease. 10(114): 1-10.

AGGARWAL, K.K., RASSOD, J.K., BEDDI, K.L., NARASHIMA, L. 1974. Commercial utilization of wild apricot kernels. Journal of Oil Technologist’s Association of India. 6: 67-69.

AHMED, F., SELIM, M.S.T., DA, A.K., CHOUDHURI, M.S.K. 2004. Anti-inflammatory and antinociceptive activities of Lippia nodiflora Linn. Pharmazie. 59: 329–330.

AKINDELE, A.J., ADEYEMI, O.O. 2007. Anti-inflammatory activity of the aqueous leaf extract of Byrsocarpus coccineus. Fitoterapia. 8: 25-28.

ASMA, B.M., KAN, T., BIRHANLI, O. 2007. Characterization of promising apricot (Prunus armeniaca L.) Genetic resources in Malatya, Turkey. Genetic Resources and Crop Evolution. 54 (1):205-212.

BABA, S.A., MALIK, S.A. 2015. Determination of total phenolic and flavonoid content, antimicrobial and antioxidant activity of a root extract of Arisaema jacquemontii Blume. Journal of Tilbian University for Science. 9(4): 449-454.

BADR, J., TAWFIK, M.K. 2014. Analytical and Pharmacological Investigation of Amygdalin in Prunus armeniaca L. Kernels. Journal of Pharmacy Research. 3(9): 2134-2137.

BENSKY, D., CLAVEY, S., STOGER, E. 2004. Chinese Herbal Medicine: Materia Medica, 3rd edition. Eastland Press: Seattle. 437–440.

CHANG, H.K., YANG, H.Y., LEE, T.H., SHIN, M.C., LEE, M.H., SHIN, M.S., KIM, C.J., KIM, O.J., HONG, S.P., CHO, S. 2005. Armeniacae semen extract suppresses lipopolysaccharide-induced expressions of cycloosygenase-2 and inducible nitric oxide synthase in mouse BV2 microglial cells. Biological and Pharmaceutical Bulletin. 28(3): 449-454.

CHEN, Q. 1993. Methodology in pharmacological study on Chinese materia medica. Beijing: 7 people’s Medical publishing house. 360.

CHEVALLIER A. 1996. The encyclopedia of medicinal plants. New York: DK Publishing.

COOK, N.C., SAMMAN, S. 1996. Flavonoids chemistry, metabolism, cardioprotective effects, and dietary sources. Nutritional Biochemistry. 7: 66-76.

DOMENJOZ, R., THEOBALD, W., STENGER, E.G., MORSDORF, K. 1995. The Effect of Anti-Inflammatory Agents on formalin edema and on the vitamin C and cholesterol content of the adrenal glands in hypophysectomized Rats. Archives internationals de pharmacodynamie et de Thérapie. 103(2-3): 341-352.

DURMAZ, G., ALPASLAN, M. 2007. Antioxidant properties of roasted apricot (Prunus armeniaca L.)Kernel. Food Chemistry. 100: 1177-1181.

GEETHA, S., SAI-RAM, M., MONGIA, S.S., SINGH, V., ILAVAZHA-GAN, G., SAWHNEY, R.C. 2003. Evaluation of antioxidant activity of leaf extract of seabuckthorn (Hippophae rhamnoides L.) on chromium (VI) induced oxidative stress in albino rats. Journal of Ethnopharmacology. 87(23): 247-251.

GEZER, I., HACISEFEROGULLARI, H., DEMIR, F. 2002. Some physical properties of Hacihaliloglu apricot pit and its kernel. Journal of Food Engineering. 56: 49-57.

GEZER, I., HACISEFEROGULLARI, H., OZCAN, M.M., ARSLAN, D., ASMA, B.M., UNVER, A. 2011. Physico-chemical properties of apricot (prunus armeniaca l.) kernels. South Western Journal of Horticulture, Biology and Environment. 2(1): 1-13.

GORNAS, P., MISINA, I., GRAVITE, I., SOLIVEN, A., KAUFMANE, E., SEGLIŅA, D. 2015. Tocochromanols composition in kernels recovered from different apricot varieties: RP-HPLC/FLD and RP-UPLC-ESI/MSn study. Natural Product Research. 29(13): 1222-1227.

HELMY, H.E. 1990. Studies on the pigments of some citrus, prune and cucurbit seed oils when processed with or without cottonseed oil. Journal of the American Oil Chemists’ Society. 67(6): 376-380.

HINZ, B., BRUNE, K., PAHL, A. 2000. Prostaglandin E2 upregulates cyclooxygenase-2 expression in lipopolysaccharide-stimulated RAW 264.7 macrophages. Biochemical and Biophysical research Communications, 272 (3), 744-748.

HOWITT, C.A., POGSON, B.J. 2006. Carotenoid accumulation and function in seeds and non-green tissues. Plant, Cell and Environment. 29: 435-445.

HWANG, D.R., KANG, Y.S., KIM, S.S., KIM, D.H., SHIN, M.K., SONG, H.J. 2003. Studies on the allergic asthma effect of semen armeniaceae amarum. The Korea Journal of Herbology. 18(2): 201-208.

HWANG, H.J, KIM, P., KIM, C.J., LEE, H.J., SHIM, I., YIN, C.S., YANG, Y., HAHM, D.H. 2008. Antinociceptive effect of amygdalin isolated from Prunus armeniaca on formalin-induced pain in rats. Biological & Pharmaceutical Bulletin. 31(8): 1559-1564.

HWANG, H.J., LEE, H., KIM, C., SHIM, I., HAHM, D. 2008. Inhibitory Effect of Amygdalin on Lipopolysaccharide-Inducible TNF- α and IL-1 β mRNA Expression and Carrageenan-Induced Rat Arthritis. Journal of Microbiology and Biotechnology. 18(10): 1641-1647.

JACOB, J., BOSOVSKI, M. 1961. Screening methods in Pharmacology Academic press INC., New York and London. 104-106.

JANSSEN, P.A., JAGENEAU, A.H. 1957. A new series of potent analgesics: dextro 2:2-diphenyl-3-methyl-4-morpholino-butyrylpyrrolidine and related amides. I. Chemical structure and pharmacological activity. Journal of Pharmacy and Pharmacology. 9(6): 381-400

JING-RONG, W., HUA, Z., ZHI-HONG, J., YUEN FAN, W., LIANG, L. 2008. In Vivo Anti-inflammatory and Analgesic Activities of a Purified Saponin Fraction Derived from the Root of Ilex pubescens, Biological and Pharmaceutical Bulletin. 31: 643-650.

JU, Y.S., KIM, K.Y., CHANG, S.H. 2004. Miyong Donguibogam. [Korean] Seoungbosa: Seoul.

KERBER, G. 1941. Pharmakolgishe Methoden Zur Auffindung Von Arzneimitten Und Gifter Und Analyse Ihrer Wirkungweise Vor. Dr. Med Leopold Ther. Wissen schaftliche Verlage Gerlarge Geese Gesellschaft M. B. H.

KESSLER, M., UBEAUD, G., JUNG, L. 2003. Anti- and pro-oxidant activity of rutin and quercetin derivatives. Journal of Pharmacy and Pharmacology. 55(1): 131-142.

LANDRUM, J.T., BONE, R.A. 2004. Dietary lutein and zeaxanthin: reducing the risk for macular degeneration. Agro Food Industry Hi-Tech. 15(6): 22-25.

OKUN, R.N., ANUAGASI, C.L., LASAGANAL, L. 1963. Screening methods in the effect of aggregation, electric shock and adrenergic blocking drugs on inhibition of the writhing syndrome. Journal of pharmacology and experimental therapeutics. 139: 107-114.

PANTHONG, A., KANJANAPOTHI, D., TAESOTIKUL, T., PHANKUMMOON, A., PANTHOG, K., REUTRAKUL, V. 2004. Anti-inflammatory activity of methanolic extracts from ventilago harmandianapierre. Journal of Ethnopharmacology. 91(2-3): 237-242.

SCHERTZ, F.M. 1923. The quantitative determination of carotene by means of the spectrophotometer and colorimeter. Journal of Agriculture Research. 26: 383-400.

SHAHADI, F., JANITHA, P.K., WANASUNDARA, P.D. 1992. Phenolic antioxidants. Critical Reviews in Food Science and Nutrition. 32(1): 67-103.

SHAO, J., SHENG, H., INOUE, H., MORROW, J.D., DUBOIS, R.N. 2000. Regulation of constitutive cyclooxygenase-2 expression in colon carcinoma cells. Journal of Biological Chemistry. 275(43), 33951-33956.

SHIMOI, K., MASUDA, S., SHEN, B., FURUGORI, M., KINZE, N. 1996. Radio-protective effects of antioxidative plant flavonoids in mice. Mutation Research/ Fundamental and Molecular mechanisms of mutagenesis. 350: 153-161.

SOOBRATTEE, M., NEERGHEEN, V.S., LUXIMON-RAMMA, A., AROMA, O.I., BAHORUN, O.T. 2005. Phenolics as potential antioxidant therapeutic agents: mechanism and actions. Mutation Research/ Fundamental and Molecular mechanisms of mutagenesis. 579(1): 200-213.

YEUM, K.J., RUSSELL, R.M. 2002. Carotenoid bioavailability and bioconversion. Annual Review of Nutrition. 22: 483-504.