A., R., Afifi, N., Yassin, N., Abdel-Rahman, R., Abd El-Rahman, S., Fayed, H. (2017). Potential Therapeutic Role of Osteopontin Inhibition in Liver Fibrosis -Induced by Thioacetamide in Rats. Journal of Applied Veterinary Sciences, 2(1), 1-8. doi: 10.21608/javs.2017.62129
Ramadan A.; Nehal A. Afifi; Nemat Z. Yassin; Rehab F. Abdel-Rahman; Sahar S. Abd El-Rahman; Hany M. Fayed. "Potential Therapeutic Role of Osteopontin Inhibition in Liver Fibrosis -Induced by Thioacetamide in Rats". Journal of Applied Veterinary Sciences, 2, 1, 2017, 1-8. doi: 10.21608/javs.2017.62129
A., R., Afifi, N., Yassin, N., Abdel-Rahman, R., Abd El-Rahman, S., Fayed, H. (2017). 'Potential Therapeutic Role of Osteopontin Inhibition in Liver Fibrosis -Induced by Thioacetamide in Rats', Journal of Applied Veterinary Sciences, 2(1), pp. 1-8. doi: 10.21608/javs.2017.62129
A., R., Afifi, N., Yassin, N., Abdel-Rahman, R., Abd El-Rahman, S., Fayed, H. Potential Therapeutic Role of Osteopontin Inhibition in Liver Fibrosis -Induced by Thioacetamide in Rats. Journal of Applied Veterinary Sciences, 2017; 2(1): 1-8. doi: 10.21608/javs.2017.62129
Potential Therapeutic Role of Osteopontin Inhibition in Liver Fibrosis -Induced by Thioacetamide in Rats
1Pharmacology Department, Faculty of Veterinary Medicine, Cairo University, Egypt.
2Pharmacology Department, Medical Division, National Research Centre, Giza, Egypt.
3Pathology Department, Faculty of Veterinary Medicine, Cairo University, Egypt.
Receive Date: 14 August 2017,
Revise Date: 07 September 2017,
Accept Date: 17 September 2017
Abstract
To examine the effect of osteopontin inhibition by tranilast on liver fibrosis, four groups of rats were used throughout this study. Group I (Control group): rats received the solvent. Liver fibrosis was induced in Groups II, III, and IV by thioacetamide (TAA; 200mg/kg, ip) twice weekly for 6 weeks. Group II served as (TAA group). Groups III and IV (Treatment groups): rats were given tranilast for 6 weeks after TAA discontinuation. Liver osteopontin (OPN), transforming growth factor-β (TGF-β1), tumor necrosis factor alpha (TNF-α), alpha-smooth muscle actin (ɑ-SMA)), reduced glutathione (GSH), superoxide dismutase (SOD) and lipid peroxidation (MDA) were measured. Additionally, expression of α-smooth muscle actin (SMA) and caspase (Cas)-3 were assigned immunohistochemically. Treatment with tranilast prevented the development of hepatic fibrosis and the activation of stellate cells, and down-regulated the expression of genes for osteopontin and osteopontin-target molecules, including TGF-β and TNF-α and α-SMA.Tranilast significantly decreased MDA and increased levels of GSH, and SOD. Our findings suggest that targeting osteopontin with tranilast represents a new mode of therapy for liver fibrosis.
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