Assessment of the peste des petits ruminants (ppr) attenuated virus produced by inoculating vero cell line at different stages of cell growth

El-Dakhly, A. T.; Youssef, M. M.; Tammam, Abeer A.; Moedham, Namaa A.

doi:10.21608/javs.2016.62123

Assessment of the peste des petits ruminants (ppr) attenuated virus produced by inoculating vero cell line at different stages of cell growth

Document Type : Original Article

Authors

¹ Rinder Pest Vaccine Research Department, Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo, Egypt

² Pox Vaccines Research Department, Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo, Egypt

10.21608/javs.2016.62123

Abstract

This work presents cultural characterization of Peste Des Petites Ruminants (PPR) virus in VERO-culture. The VERO cells are currently considered as an acceptable cell substrate to produce a wide range of viruses. This study evaluates the best time for inoculation of PPR virus on VERO cell cultures; the study proved that the optimum time was 24 hours after subculture of VERO cell line using MOI 2: 1. It was also found that the best time of harvstation of virus fluid of PPR was 9^th day post inoculation to reach the best titre 6 log10 TCID50 /ml.

Keywords

Main Subjects

Virology

References

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