Atiyah, A., L.M., A. (2024). Impact of Fabricated Coral Shell Hydroxyapatite Powder and Autologous Plasma Rich- fibrin in Remodeling of the Mandibular Bone Critical Size Defect in Dogs: Histopathological and Immunohistochemical Study. Journal of Applied Veterinary Sciences, 9(2), 111-119. doi: 10.21608/javs.2024.266431.1312
Ali Ghazi Atiyah; Alkattan L.M.. "Impact of Fabricated Coral Shell Hydroxyapatite Powder and Autologous Plasma Rich- fibrin in Remodeling of the Mandibular Bone Critical Size Defect in Dogs: Histopathological and Immunohistochemical Study". Journal of Applied Veterinary Sciences, 9, 2, 2024, 111-119. doi: 10.21608/javs.2024.266431.1312
Atiyah, A., L.M., A. (2024). 'Impact of Fabricated Coral Shell Hydroxyapatite Powder and Autologous Plasma Rich- fibrin in Remodeling of the Mandibular Bone Critical Size Defect in Dogs: Histopathological and Immunohistochemical Study', Journal of Applied Veterinary Sciences, 9(2), pp. 111-119. doi: 10.21608/javs.2024.266431.1312
Atiyah, A., L.M., A. Impact of Fabricated Coral Shell Hydroxyapatite Powder and Autologous Plasma Rich- fibrin in Remodeling of the Mandibular Bone Critical Size Defect in Dogs: Histopathological and Immunohistochemical Study. Journal of Applied Veterinary Sciences, 2024; 9(2): 111-119. doi: 10.21608/javs.2024.266431.1312
Impact of Fabricated Coral Shell Hydroxyapatite Powder and Autologous Plasma Rich- fibrin in Remodeling of the Mandibular Bone Critical Size Defect in Dogs: Histopathological and Immunohistochemical Study
1Department of Surgery and Obstetrics, College of Veterinary Medicine, University of Tikrit, Iraq
2Department of Surgery and Theriogenology, College of Vet., Med., University of Mosul, Iraq
Receive Date: 30 January 2024,
Revise Date: 01 March 2024,
Accept Date: 10 March 2024
Abstract
Histopathological and immunohistochemical assessment of fabricated coral shell hydroxyapatite (CSHA) and plasma rich fibrin (PRF) in remodeling of the induced critical size defect of the mandibular bone in the dogs: Twenty-seven adult dogs of both sexes were included and equally divided into three equal groups: control, plasma-rich fibrin (PRF) and hydroxyapatite group (CSHA). The experimental mandibular bone defect was induced in a circular shape, and the dimensions of the defect were 14×5mm. Evaluation of the healing progress of the defect and associated macroscopical, histopathological, and Immunohistological findings was recorded in all studied groups at 7, 15, and 30 days post-operatively. Macroscopically, the healing was evaluated by the presence of new bone tissue filling the bone gap defect in all groups during different follow-up periods. In the plasma-rich fibrin (PRF) group, the gap was highly filled with hard, firm tissues that filled all borders and the centre of the induced gap in comparison with the coral shell hydroxyl apatite group (CSHA), which is partially filled with hard tissue. Histopathologically, the progress of healing in the PRF group was represented by the presence of highly mature connective tissue and new woven bone formation at seven days and well-developed mature bone inside defective bone at 15 and 30 days post-operatively, whereas in the CSHA group, the results were represented by the occlusion of highly mature connective tissue and new woven bone formation inside the induced hole at 15 and 30 days post-operatively. At 30 days post-surgery, in the control group, there was the presence of newly formed woven bone surrounded by the edge of the mandible bone. The immunohistochemical expression of the alkaline phosphatase (ALP) in the mandible bone at 30 days PS in the control group was represented by weak positive expression, while mild positive expression was indicated in the CSHA group and moderate positive expression in the PRF group. In conclusion, this research exhibited the role of both CSHA and PRF in improving the healing process of defective mandible bones, with a clear superiority of the beneficial value of using PRF. The histopathological and immunohistochemistry assessments emphasize these results.
ABDULGHANI, S., and MITCHELL, G.R., 2019. Biomaterials for in situ tissue regeneration: A review. Biomolecules., 19,9(11):750. https://doi.org/10.3390/biom9110750
ALAM, K., AL-GHAITHI, A., PIYA, S., and SALEEM, A., 2019. In-vitro experimental study of histopathology of bone in vibrational drilling. Medical engineering and physics, 67, 78-87. https://doi.org/10.1016/j.medengphy.2019.03.013
ALKATTAN, L.M., and HELALl, M., 2013. Effects of ketamine-xylazine and propofol-halothane anesthetic protocols on blood gases and some anesthetic parameters in dogs. Veterinary World, 6(2):95-99. http://dx.doi.org/10.5455/vetworld.2013.95-99
ALLAWI, A.H., ALKATTAN, L.A., and AL IRAQI. O.M., 2019. Clinical and ultrasonographic study of using autogenous venous graft and platelet-rich plasma for repairing Achilles tendon rupture in dogs. Iraqi Journal of Vetrinary Science. 33(2):453-460. https://doi.org/10.33899/ijvs.2019.163199
ATIAH, A. 2018. Use of eggshell hydroxyapatite implant in repair of radial bone defects in rabbits . [master's thesis]. Baghdad: University of Baghdad; pp:34.
BIGHAM, A., DEHGHANI, S., SHAFIEI, Z., and TORABI N.S., 2008. Xenogenic demineralized bone matrix and fresh autogenous cortical bone effects on experimental bone healing: radiological, histopathological and biomechanical evaluation. Journal of Orthopedic. Traumatol., 9(2):73-80. https://doi.org/10.1007/s10195-008-0006-6
CHANDRAN, P., and SIVADASB, A., 2014. Platelet-rich fibrin: Its role in periodontal regeneration. Saudi Journal for Dental Research.,5(2):117-122. https://doi.org/10.1016/j.ksujds.2013.09.001
CINTI, F., ROSSANESE, M., BURACCO, P., PISANI, G., VALLEFUCO, R., MASSARI, F., and CANTATORE, M., 2021. Complications between ventral and lateral approach for mandibular and sublingual sialoadenectomy in dogs with sialocele. Veterinary Surgery., 50(3):579-587. https://doi.org/10.1111/vsu.13601
FELIPE R.S., BRUNO, W.M., SIDNEY, W.G.S., IVIA, de P.C., PEDRO, P.R., JOSE, S.C.J., MARIO, T.J., and LUIS, G.G., 2020. Caprine demineralized bone matrix (DBMc) in the repair of non-critical bone defects in rabbit tibias. A new bone xenograft Acta Cirurigca Brasilia .,35(8). https://doi.org/10.1590/s0102-865020200080000001
GAO, C., PENG, S., FENG, P., and SHUAI, C., 2017. Bone biomaterials and interactions with stem cells. Bone research, 5:1-33. https://doi.org/10.1038/boneres.2017.59
GOBBI, G., and VITALE, M., 2012. Platelet-rich plasma preparations for biological therapy: applications and limits. Operative Techniques in Orthopaedics.,22:10-15. https://doi.org/10.1053/j.oto.2012.01.002
HUSSEIN, A.A., and TAQA, G.A., 2021. The impact of natural calcium carbonate and Ubiquinone on bone mineral density in rabbits. Journal of Applied Veterinary Sciences, 6(4):15-22. https://doi.org/10.21608/javs.2021.87062.1091
HOSHI, K., AMIZUKA, N., ODA, K., IKEHARA, Y., and OZAWA, H., 1997. Immunolocalization of tissue non-specific alkaline phosphatase in mice. Histochem Cell Biol., 107, 183–191. https://doi.org/10.1007/s004180050103
HUH, J.Y., CHOIi, B.H., KIM, B.Y., LEE, S.H., ZHU, S.J., and JUNG, J.H. 2005. Critical size defect in the canine mandible. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiologyand Endodontology.,100(3):296-301. https://doi.org/10.1016/j.tripleo.2004.12.015
JASMINE, S., and KRISHNAMOORTHY, R., 2022. Biodegradable Materials for Bone Defect Repair. Biodegradable Materials and Their Applications, 457-470 . https://doi.org/10.1186/s40779-020-00280-6
JIN,Y.H., BYUNG-Ho, C., BYUNG, Y.K, SEOUNG-Ho Lee, and SHI-J.Z,JAE-H.J., 2005. Critical size defect in the canine mandible. Oral Surg Oral Med Oral Pathol Oral Radiol. Endod.,100(3), 0–301. https://doi.org/10.1016/j.tripleo.2004.12.015
KALANTAR, A., and KHORVASH, B., 2006. Repair of skin covering osteoradionecrosis of the mandible with the fas-ciocutaneous supraclavicular artery island ap: case re- port. J.J Cranio Maxillo Facial Surgery ., 34(7):440-2 https://doi.org/10.1016/j.jcms.2005.05.006
KOMATSU, D.E., BRUNE, K.A., LIU, H., SCHMIDT, A.L., HAN, B., ZENG, Q.Q., and GEISER, A.G., 2009. Longitudinal in vivo analysis of the region-specific efficacy of parathyroid hormone in a rat cortical defect model. Endocrinology, 150(4): 1570-1579. https://doi.org/10.1210/en.2008-0814
MAREI, H.F, MOHMOOD, K., and ALMAS, K., F.D.S. (RCSEd)., 2017. Critical Size Defects for Bone Regeneration Experiments in the Dog Mandible: A Systematic Review .Implant Dintersry ., 27(1). https://doi.org/10.1097/id.0000000000000713
MOHAMMED, F.M., ALKATTAN, L.M., AHMED, M.S., and THANOON M.G., 2023. Evaluation the effect of high and low viscosity Nano-hydroxylapatite gel in repairing of an induced critical-size tibial bone defect in dogs: Radiolographical study. Journal of Applied Veterinary Sciences,8(3):105-110. https://dx.doi.org/10.21608/javs.2023.215990.1239
MOHAMMED, F.M., ALKATTAN, L., and M.ISMAIL, H.K., 2022. Histopathological and serological assessment of using rib lamb xenograft reinforced with and without hydroxyapatite nano gel for reconstruction tibial bone defect in dogs. Iraqi Journal of Vetrinary. Science, 36, Supplement I, (69-76). https://doi.org/10.33899/ijvs.2022.135366.2473
NAJI, A.H., Al-WATTAR, W.T., and TAQA, G.A., 2022. The Effect of Xylitol on Bone Alkaline Phosphatase Serum Level and Bone Defect Diameter in Rabbits. Journal of Applied Veterinary Sciences, 7(1), 6-10. https://dx.doi.org/10.21608/javs.2021.97815.1105
NUGRHA, A.P., NARMADA, I.B., ERNAWATI, D.S., DINARYANTI, A., HENDRIANTO, E., RIAWAN, W., and RANTAM, F.A., 2018. Bone alkaline phosphatase and osteocalcin expression of rat's Gingival mesenchymal stem cells cultured in platelet-rich fibrin for bone remodeling (in vitro study). European Journal Dent.,12(04), 566-573. https://doi.org/10.4103/ejd.ejd_261_18
POLINI, A., PISIGNANO, D., PARODI, M., QUARTO, R., and SCAGLIONE, S., 2011. Osteoinduction of human mesenchymal stem cells by bioactive composite scaffolds without supplemental osteogenic growth factors. Plos one, 6(10), e26211. https://doi.org/10.1371/journal.pone.0026211
ROUDANA, M.A., RAMESHA, S.,NIAKANB, A.,WONGA, Y.H., ZAVAREHA, M.A., CHANDRANC, H. and SUTHARSINIF, U. 2017. Thermal phase stability and properties of hydroxyapatite derived from bio-waste eggshells. Journal of Ceramic Processing Research., 18(1): 69-72. https://www.researchgate.net/publication/316062556.
SADEK, A.A., ABD-ELKAREEM, M., ADBELHAMID, H.N., MOUSTAFA, S., and HUSSEIN, K., 2023. Repair of critical-sized bone defects in rabbit femurs using graphitic carbon nitride (g-C3N4) and graphene oxide (GO) nanomaterials. Scientific Reports, 13(1):5404. https://doi.org/10.1038/s41598-023-32487-7
SHADJOU, N. and HASANZADEH,M. 2015. Bone tissue engineering using silica-based mesoporous nanobiomaterials: Recent progress. Materials Science and Engineering., 55:401-409. https://doi.org/10.1016/j.msec.2015.05.027
SHARMA, A., INGOLE, S., DESHPANDE, M., RANADIVE, P., SHARMA, S., KAZI, N., and RAJURKAR, S., 2020. Influence of platelet-rich fibrin on wound healing and bone regeneration after tooth extraction: A clinical and radiographic study. J Oral Biol Craniofac Research , 10(4): 385-390. https://doi.org/10.1016/j.jobcr.2020.06.012
SHARMA, U., PAL, D., and PEASAD, R., 2014. Alkaline phosphatase: an overview. Indian Journal Clinical Biochemical., 29: 269-278. https://doi.org/10.1007%2Fs12291-013-0408-y
STERNER, R.M., KREMER, K.N., DUDAKOVIC, A., WESTENDROF, J.J., VAN WIJNEN, A.J., and HEDIN, K. E. 2018.Tissue-Nonspecific Alkaline Phosphatase Is Required for MC3T3 Osteoblast–Mediated Protection of Acute Myeloid Leukemia Cells from Apoptosis. The Journal .of Immunology., 201(3):1086-1096. https://doi.org/10.4049/jimmunol.1800174
SURVANA, S.K., LAYTON, C., BANCROFT, J.D., and BANCROF S., 2013.Theory and Practice of Histological Techniques.7th ed. Churchill Livingstone Press ,USA. P. 12-32 .
TAMBELLA, A.M, BARTOCETTI,F., ROSSI, G, GALOSI, L., CATONE,G., FALCONE,A., and VULLO, C., 2020. Effects of Autologous Platelet-Rich Fibrin in Post-Extraction Alveolar Sockets: A Randomized, Controlled Split-Mouth Trial in Dogs with Spontaneous Periodontal Disease. Animals, 10(8):1343. https://doi.org/10.3390/ani10081343
WANG, X., ZHANG, Y., CHOUKROUM, J., GHANAATI, S., and MIRON, R.J., 2018. Effects of an injectable platelet-rich fibrin on osteoblast behavior and bone tissue formation in comparison to platelet-rich plasma. Platelets, 29(1):48-55. https://doi.org/10.1080/09537104.2017.1293807
XIAN, C.J., ZHOU, F.H., MCCARTY, R.C., and FOSTER, B.K., 2004. Intramembranous ossification mechanism for bone bridge formation at the growth plate cartilage injury site. Journal of Orthopedic Research ., 22(2):417-426. https://doi.org/10.1016/j.orthres.2003.08.003
ZEBON, S.H., EESA, MJ., and BAHAA,F.H., 2020. Efficacy of Nano Composite Porous 3D Scaffold of Crab Shell and Al-Kharit,Histological and Radiological for Bone Repair in Vivo. Iraq Jounal of Veterinary. Medicine., 44(2),15–24. https://doi.org/10.30539/ijvm.v44i2.973
ZEDAN, I.A., ALKATTAN, L.A., AL IRAQI, O.M., 2022. An evaluation of Aloe vera leaves gel with polypropylene mesh to repair of ventrolateral abdominal hernia in rams . Iraqi Journal. Vetrinary. Science., 36:19-25. . https://doi.org/10.33899/ijvs.2022.134989.2430
ZEDAN, I.A., ALKATTAN, L.A., and Al-MAHMOOD, S.S., 2023. Histopathological and immunohistochemical assessment of the using platelets rich fibrin to reinforce ventral hernioplasty in the sheep modelIraqi . Iraqi Journal. Vetrinary. Science .,37, (4): 821https://www.vetmedmosul.com/article_180501.html
ZHANG, Q., WU, W., QIAN, C., XIAO, W., ZHU, H., and GUO,J.,CUI,W., 2019. Advanced biomaterials for repairing and reconstruction of mandibular defects. Materials Science and Engineering.,103:109858. https://doi.org/10.1016/j.msec.2019.109858
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