The Impacts of Teriparatide Treatment on Bone Alkaline Phosphatase, Calcium and Phosphate Serum Levels in Rats

Ahmed, Duaa; Al-Wattar, Wael; Taqa, Ghada A.

doi:10.21608/javs.2023.240748.1281

The Impacts of Teriparatide Treatment on Bone Alkaline Phosphatase, Calcium and Phosphate Serum Levels in Rats

Document Type : Original Article

Authors

¹ Ministry of Health, Nineveh Health Directorate, Mosul, Iraq

² Department of Oral and Maxillofacial Surgery, College of Dentistry, University of Mosul , Mosul, Iraq

³ Professor in veterinary pharmacology and toxicology, College of Dentistry , Mosul university , Iraq

10.21608/javs.2023.240748.1281

Abstract

Trauma, tooth loss, cancer surgery, congenital malformations, periodontal disease, and oral-maxillofacial surgery are all major causes of bone loss, deformity, or fracture. Bone healing is a physiologically complicated process that also incorporates mechanical factors. Too many doubts concerning bone regeneration and the conditions that promote it have persisted for far too long. To assess the impact of systemic teriparatide therapy on mandibular bone defects healing.Forty male albino rats were randomly divided into two groups: the control group and the treatment group, with 20 rats per group. All rats had the same surgical technique. A small hole was created in the mandible, measuring 3 mm in diameter and 3 mm in depth, and then left unoccupied. The treatment group received a daily subcutaneous injection of Teriparatide at a dose of 10 µg/kg. Animals were subjected to euthanasia at four distinct time intervals (7, 14, 21, and 28) days. Biochemical analyses were done. Teriparatide was shown to elevate serum levels of bone alkaline phosphatase (BALP), elevate serum calcium concentration levels, and lower serum phosphate. There were statistically significant differences in the levels of BALP between the control group and the treated group at 7, 14, and 21 days after the surgical procedure and there was a statistically significant difference in serum calcium levels between the treated group and the control group on day 14 of the experiment. The findings of this research suggest that the systemic administration of teriparatide resulted in an acceleration of bone healing. This was evidenced by a notable elevation in bone alkaline phosphatase levels as seen in the biochemical analysis. Teriparatide slightly elevated serum calcium levels and lowered serum phosphate levels because it is a synthetic parathyroid hormone.

Keywords

Main Subjects

Surgery

References

BHUYAN, L., DASH, K. C., RAGHUVANSHI, M., and RAMACHANDRA, S., 2017. Teriparatide - potential boon in dentistry. World Journal of Pharmaceutical Research , 7 (2), 303-316. https://www.wjpr.net/abstract_show/8841.

BLICK, S. K., DHILLON, S., and KEAM, S. J., 2008. Teriparatide: a review of its use in osteoporosis.Drugs, 68(18),2709-37. https://doi.org/10.2165/0003495-200868180-00012.

BONNICK, S., De VILLIERS, T., ODIO, A., PALACIOS,S., CHAPURLATE, R., DASILVA, C., and GURNER, D., 2013. "Effects of odanacatib on BMD and safety in the treatment of osteoporosis in postmenopausal women previously treated with alendronate: a randomized placebo-controlled trial. The Journal of Clinical Endocrinology & Metabolism, 98(12), 4727-4735. https://doi.org/10.1210/jc.2013-2020.

CANALIS, E. 2018. MANAGEMENT OF ENDOCRINE DISEASE: Novel anabolic treatments for osteoporosis. European journal of endocrinology, 178(2), R33-R44. https://doi.org/10.1530/EJE-17-0920.

COLLOCA, G., DI CAPUA,B., BELLIENI, A., FUSCO, D., CICIARELLO, F. L., TAGLIAFERRI, L., and BALDUCCI, L., 2020. Biological and functional biomarkers of aging: definition, characteristics, and how they can impact everyday cancer treatment. Current Oncology Reports, 22(11),115. https://doi.org/10.1007/s11912-020-00977-w.

HUSSEIN, A. A., and TAQA, G.A., 2021. The impact of natural calcium carbonate and Ubiquinone on bone mineral density in rabbits. Journal of Applied Veterinary Sciences, 6 (4) , 15-22 . https://dx.doi.org/10.21608/javs.2021.87062.1091.

KESKINRUZGAR, A., YANIK, S., ARAS, M., and ÇETINER , S., 2015. Teriparatide [parathyroid hormone (1-34)] and dentistry. Acta Odontol Turca, 32, 165. DOI: http://dx.doi.org/10.17214/aot.74775.

LE HENAFF, C., and Partridge,N.C. 2020. Parathyroid Hormones. Hormonal Signaling in Biology and Medicine, 507-529. https://doi.org/10.1016/B978-0-12-813814-4.00022-5.

MAHMOOD, H. R., and TAQA, A. G., 2022. Systemic Effect of Metformin on Bone Healing at Bone Defect in Rabbits Using Radiographical and Serum Alkaline Phosphatase Assessment. Vet Sci Pract, 17(3), 91-97 l . DOI: 10.5152/ 2022.223353.

MOHAMMED , Z. S., TAQA , G.A., and SULAIMAN, M.S., 2022. Evaluating thelocal and systemic effects of silicon dioxide on healing of mandibular bone in rabbits. AIP Conference Proceedings, AIP Publishing, Vol. 2547, No. 1) https://doi.org/10.1063/5.0116015.

NAJI, A. H., Al-WATTER ,W.T., and TAQA,G.A., 2022. The Effect of Xylitol on Bone Alkaline Phosphatase Serum Level and Bone Defect Diameter in Rabbits. Journal of Applied Veterinary Sciences, 7(1), 6-10. https://dx.doi.org/10.21608/javs.2021.97815.1105.

SARMA, I. D., BRAHMA,D.K., and BANERJEE, P., 2021. Teriparatide in post- menoposal osteoprosis-A new revoluation for osteoporosis. World Journal of Pharmaceutical Research,10( 12), 976-982. DOI: 10.20959/wjpr202112-21865.www.wjpr.net.

SHARMA , U., PAL, D., and PRASAD, R., 2014. Alkaline phosphatase: an overview." Indian journal of clinical biochemistry, 29, 269-278. https://doi.org/10.1007/s12291-013-0408-y.

TAQA, G. A., Al-HUSSARY, B. N., and KASHMOLA, N. T., 2023. Preparing of Nano-Hydroxyapatite from Seashell and Mixed with Gold to Repair Bony Defect of Mandibular Bone in Rabbits. Iraqi Journal of Industrial Research, 10(1), 120-130.‏ https://doi.org/10.53523/ijoirVol10I1ID278.

VIMARLRAJ, S. 2020. Alkaline phosphatase: Structure, expression and its function in bone mineralization. Gene, 5,(754),144855. https://doi.org/10.1016/j.gene.2020.144855

WAWRZYNIAK, A., and BALAWENDER, K., 2022. Structural and metabolic changes in bone. Animals, 12(15), 1946. https://doi.org/10.3390/ani12151946.

YOSHIGA, D., YOSHIOKA, I., HABU, M., SASAGURI, M., and TOMINAGA, K., 2022. Effective ancillary role and long-term course of daily or weekly teriparatide treatment on refractory medication-related osteonecrosis of the jaw: a clinical case series. British Journal of Oral and Maxillofacial Surgery, 60(5), 604-609. https://doi.org/10.1016/j.bjoms.2021.10.004.

YOUNG, K., BEGGS, M. R., GRIMBLY, C., and ALEXENDER, R. T., 2022. Regulation of 1 and 24 hydroxylation of vitamin D metabolites in the proximal tubule. Experimental Biology and Medicine, 247(13), 1103-1111.‏ https://doi.org/10.1177/15353702221091982.

ZANDI, M., DEHGHAN , A., GHEYSARI, F., REZAEIAN, L., and MEZERJI, N. M. G., 2019. Evaluation of teriparatide effect on healing of autografted mandibular defects in rats. Journal of Cranio-Maxillofacial Surgery, 47(1), 120-126. https://doi.org/10.1016/j.jcms.2018.11.015.