Ultrasonographic Anatomy, Electrophysiological and Histological Studies on Sciatic Nerve in Dog

Khaled, Mona M.; Ibrahium, Asmaa M.; Abdelgalil, Ahmed I.; El-Saied, Mohamed A.; Abouquerin, Nagy; El-Bably, Samah H.

doi:10.21608/javs.2023.232947.1267

Ultrasonographic Anatomy, Electrophysiological and Histological Studies on Sciatic Nerve in Dog

Document Type : Original Article

Authors

¹ Department of Anatomy & Embryology, Faculty of Veterinary Medicine, Cairo University, Egypt

² Department of Surgery, Anaesthesiology, and Radiology, Faculty of Veterinary Medicine, Cairo University, Egypt

³ Department of Pathology, Faculty of Veterinary Medicine, Cairo University, Egypt

⁴ Department of Physiology, Faculty of Medicine, Ain Shams University, Egypt

⁵ Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt

10.21608/javs.2023.232947.1267

Abstract

The sciatic nerve is the major mixed nerve of the canine hind limb. It supplies most of the hind limb musculature. Sciatic nerve morphology and ultrasound guide surgeons and clinicians who contribute to the neurological examination of the sciatic nerve. So, the current study aimed to provide a fully descriptive ultrasonographic anatomy of the normal sciatic nerve in dogs and to set the motor conduction reference values of this nerve. Ultrasonography and neurography were applied to five adult healthy male dogs; two of them were euthanized for morphological and histological studies to describe the origin, branches, and innervated muscles of the sciatic nerve. The sciatic nerve originates from the ventral branches of the L6, L7, and S1 spinal nerves, splitting into muscular and sural branches, and is terminated by the perineal and tibial nerves. The normal sciatic nerve appears to have a hypoechoic core surrounded by hyperechoic bundles. The mean of the estimated electrophysiological parameters of the tibial and common fibular nerves was 2.20, 4.88, 20.08, 67, and 2.50, 7.78, 14.82, and 72.80 for the duration, latency, amplitude, and conduction velocity, respectively. Histological evaluation of the sciatic nerve revealed several nerve fasciculi surrounded by an epineural connective tissue sheath. The study provides a professional descriptive evaluation of the ultrasonographic anatomy of the sciatic nerve from its origin until divergence at the distal femur, which facilitates sciatic nerve evaluation for beginners and practitioners. The study also provides a full description of the neurographical and histological data of the normal sciatic nerve in dogs, which can be used as reference values for comparison with dogs that suffered sciatic nerve injuries.

Keywords

Main Subjects

Anatomy and Histology

References

ABRAHAM, L., MITTEN, R., BECK, C., CHARLES, J., and HOLLOWAY, S., 2003. Diagnosis of sciatic nerve tumour in two dogs by electromyography and magnetic resonance imaging. Australian veterinary journal,81(1‐2), 42-46. https://doi.org/10.1111/j.1751-0813.2003.tb11421.x

ASATO, F., BUTLER, M., BLOMBERG, H., and GORDH, T., 2000. Variation in rat sciatic nerve anatomy: implications for a rat model of neuropathic pain. Journal of the Peripheral Nervous System, 5(1), 19-21. https://doi.org/10.1046/j.1529-8027.2000.00155.x

BENIGNI, L., CORR, S. A., and LAMB, C. R., 2007. Ultrasonographic assessment of the canine sciatic nerve. Veterinary Radiology & Ultrasound, 48(5), 428-433. https://doi.org/10.1111/j.1740-8261.2007.00273.x

BENNETT, D. 1976. An anatomical and histological study of the sciatic nerve, relating to peripheral nerve injuries in the dog and cat. Journal of Small Animal Practice, 17(6), 379-386. https://doi.org/10.1111/j.1748-5827.1976.tb06974.x

CAMPOY, L., BEZUIDENHOUT, A. J., GLEED, R. D., MARTIN-FLORES, M., RAW, R. M., SANTARE, C. L., JAY, A. R., and WANG, A. L., 2010. Ultrasound-guided approach for axillary brachial plexus, femoral nerve, and sciatic nerve blocks in dogs. Veterinary anaesthesia and analgesia, 37(2), 144-153. https://doi.org/10.1111/j.1467-2995.2009.00518.x

CASTRO, J., NEGREDO, P., and AVENDAÑO, C., 2008. Fiber composition of the rat sciatic nerve and its modification during regeneration through a sieve electrode. Brain research, 1190, 65-77. https://doi.org/10.1016/j.brainres.2007.11.028

CHATO-ASTRAIN, J., GARCÍA-GARCÍA, O. D., CAMPOS, F., SÁNCHEZ-PORRAS, D., and CARRIEL, V., 2022. Basic Nerve Histology and Histological Analyses Following Peripheral Nerve Repair and Regeneration. Peripheral Nerve Tissue Engineering and Regeneration, 151. https://doi.org/10.1016/j.bbrc.2020.04.094

COSTA-FARRÉ, C., BLANCH, X. S., CRUZ, J. I., and FRANCH, J., 2011. Ultrasound guidance for the performance of sciatic and saphenous nerve blocks in dogs. The Veterinary Journal, 187(2), 221-224. https://doi.org/10.1016/j.tvjl.2009.10.016

CUDDON, P. A. 2002. Electrophysiology in neuromuscular disease. Veterinary Clinics: Small Animal Practice,32(1), 31-62. https://doi.org/10.1016/S0195-5616(03)00079-2

DAYER, T., ROHRBACH, H., FORTERRE, S., STOFFEL, M. H., and FORTERRE, F., 2017. Investigation of sciatic nerve surgical anatomy in dogs and cats: a comparative cadaveric study. International Journal of Veterinary Science, 6(3), 131-135. http://dx.doi.org/10.7892/boris.108853

DE LAHUNTA, A., GLASS, E., and KENT, M., 2009. Lower motor neuron: spinal nerve, general somatic efferent system. Veterinary neuroanatomy and clinical neurology, 3, 119-120. https://doi.org/10.1016/j.mehy.2007.09.011

ECHEVERRY, D. F., GIL, F., LAREDO, F., AYALA, M. D., BELDA, E., SOLER, M., and AGUT, A., 2010. Ultrasound-guided block of the sciatic and femoral nerves in dogs: a descriptive study. The Veterinary Journal, 186(2), 210-215. https://doi.org/10.1016/j.tvjl.2009.08.005

EL-BABLY, S., and ABDELGALIL, A., 2018. Ultrasonographic anatomy of the equine carpal region (Equus caballus). International Journal of Veterinary Science, 7(1), 44-49.

EVANS, H. E., and DE LAHUNTA, A., 2013. Miller's anatomy of the dog-E-Book. Elsevier health sciences.

GEUNA, S., RAIMONDO, S., RONCHI, G., DI SCIPIO, F., TOS, P., CZAJA, K., and FORNARO, M., 2009. Histology of the peripheral nerve and changes occurring during nerve regeneration. International review of neurobiology, 87, 27-46. https://doi.org/10.1016/S0074-7742(09)87003-7

GIZA, E., NICPON, J., and WRZOSEK, M., 2014. Clinical and Electrodiagnostic Findings in A Cohort of 61 Dogs with Peripheral Nervous System Diseases-A Retrospective Study. Pakistan Veterinary Journal, 34(2).

GOJRI, B. A., PARRAH, J.-U.-D., DAR, M.-U.-D., AHMAD, R. A., RESHI, P. A., and FAROOQ, U. B., 2022. Gross and sonographic anatomy of sciatic nerve in fecB Sheep. SKUAST Journal of Research, 242), 242-247. http://dx.doi.org/10.5958/2349-297X.2022.00044.7

HARO, P., GIL, F., LAREDO, F., AYALA, M. D., BELDA, E., SOLER, M., and AGUT, A., 2011. Ultrasonographic study of the feline sciatic nerve. Journal of feline medicine and surgery, 13(4), 259-266. https://doi.org/10.1016/j.jfms.2010.12.004

HARO, P., LAREDO, F., GIL, F., BELDA, E., AYALA, M. D., SOLER, M., and AGUT, A., 2012. Ultrasound-guided block of the feline sciatic nerve. Journal of feline medicine and surgery, 14(8), 545-552. https://doi.org/10.1177/1098612X12443749

HUDSON, J., STEISS, J., BRAUND, K., and TOIVIO‐KINNUCAN, M., 1996. Ultrasonography of peripheral nerves during Wallerian degeneration and regeneration following transection. Veterinary Radiology & Ultrasound,37(4), 302-312. https://doi.org/10.1016/j.jneumeth.2010.02.023

KHALED, M. M., IBRAHIUM, A. M., ABDELGALIL, A. I., EL-SAIED, M. A., and EL-BABLY, S. H., 2023. Regenerative Strategies in Treatment of Peripheral Nerve Injuries in Different Animal Models. Tissue Engineering and Regenerative Medicine, 1-39. https://doi.org/10.1007/s13770-023-00559-4

KIM, S. Y., GEORGY, J. S., and IVANOV, Y. O., 2019. Clinical Nerve Function Studies and Imaging. In Academic Pain Medicine (pp. 105-110). Springer. https://doi.org/10.1007/978-3-030-18005-8

KIMURA, J. (2001). Principles and variation of nerve conduction studies. Electrodiagnosis in diseases of nerve and muscle: principles and practice. https://doi.org/10.1002/ana.410160402

MAHLER, S. P., and ADOGWA, A. O., 2008. Anatomical and experimental studies of brachial plexus, sciatic, and femoral nerve-location using peripheral nerve stimulation in the dog. Veterinary anaesthesia and analgesia, 35(1), 80-89. https://doi.org/10.1111/j.1467-2995.2007.00356.x

MAROLF, V., ROHRBACH, H., BOLEN, G., VAN WIJNSBERGHE, A.-S., and SANDERSEN, C.M., 2019. Sciatic nerve block in dogs: description and evaluation of a modified ultrasound-guided parasacral approach. Veterinary anaesthesia and analgesia, 46(1), 106-115. https://doi.org/10.1016/j.vaa.2018.10.003

MARTINOLI, C., BIANCHI, S., DAHMANE, M. H., PUGLIESE, F., BIANCHI-ZAMORANI, M., and VALLE, M., 2002. Ultrasound of tendons and nerves. European radiology, 12(1), 44-55. https://doi.org/10.1007/s00330-001-1161-9

MICIELI, F., CHIAVACCINI, L., MENNONNA, G., DELLA VALLE, G., PRISCO, F., MEOMARTINO, L., and VESCE, G., 2021. An ultrasound-guided subparaneural approach to the sciatic nerve in the dog: a cadaver study. Veterinary anaesthesia and analgesia, 48(1), 107-115. https://doi.org/10.1016/j.vaa.2020.06.008

MORTARI, A. C., QUITZAN, J. G., BRANDÃO, C. V. S., and RAHAL, S. C., 2018. Sciatic Nerve Injection Palsy in a Dog: Electrodiagnostic Testing and Microsurgical Treatment. Acta Scientiae Veterinariae, 46, 4. https://doi.org/10.22456/1679-9216.86738

PLATT, S. 2010. Small Animal Neurology: An Illustrated Text. Schlütersche.

SIEVERT, C., RICHTER, H., GASCHO, D., KIRCHER, P. R., and CARRERA, I., 2017. 3 Tesla magnetic resonance imaging study of the normal canine femoral and sciatic nerves. Veterinary Radiology & Ultrasound, 58(5), 598-606. https://doi.org/10.1111/vru.12511

SILVESTRI, E., MARTINOLI, C., DERCHI, L. E., BERTOLOTTO, M., CHIARAMONDIA, M., and ROSENBERG, I., 1995. Echotexture of peripheral nerves: correlation between US and histologic findings and criteria to differentiate tendons. Radiology, 197(1), 291-296. https://doi.org/10.1148/radiology.197.1.7568840

TOIJALA, T. M., CANAPP, D. A., and CANAPP, S. O., 2021. Ultrasonography Findings in the Proximal Sciatic Nerve and Deep Gluteal Muscles in 29 Dogs With Suspected Sciatic Neuritis. Frontiers in Veterinary Science, 8. https://doi.org/10.3389%2Ffvets.2021.704904

UEYAMA, T. 1978. The topography of root fibres within the sciatic nerve trunk of the dog. Journal of Anatomy, 127(Pt 2), 277.

VLEGGEERT-LANKAMP, C. L. 2007. The role of evaluation methods in the assessment of peripheral nerve regeneration through synthetic conduits: a systematic review. Journal of neurosurgery, 107(6), 1168-1189. https://doi.org/10.3171/JNS-07/12/1168

WALKER, T., REDDING, R., and BRAUND, K., 1979. Motor nerve conduction velocity and latency in the dog. American journal of veterinary research, 40(10), 1433-1439. PMID: 525865.