Identification of Histopathological Changes Induced by Amitraz in Rats

Mohammed, Zainab Taha; Nabi, Rizgar Khalid; Mousa, Yaareb Jaafar; Mahmood, Mahmood Basher

doi:10.21608/javs.2022.154375.1169

Identification of Histopathological Changes Induced by Amitraz in Rats

Document Type : Original Article

Authors

¹ Department of Internal Medicine, Surgery and Pharmacology, College of Veterinary Medicine, University of Dohuk, Duhok, Iraq.

² Department of Pathology and Microbiology, Collage of Veterinary Medicine, University of Duhok, Duhok, Iraq.

³ Department of Physiology, Biochemistry and Pharmacology, College of Veterinary Medicine, University of Mosul, Mosul, Iraq.

10.21608/javs.2022.154375.1169

Abstract

Amitraz is used worldwide as a pesticide, it produces toxic effects when misused. The peresent study examined the histopathological changes of amitraz in the hepatocyte and kidney tissues of the laboratory albino rats. Thirty-six albino rats were used and they were randomly divided into six groups. Amitraz was given through oral gavage at different doses as follows: The control group (group one) received 5ml/kg of normal saline, group two received 100mg/kg, group three had 250mg/kg, group four received 500mg/kg , organs extraction from the rats (livers and kidneys) were performed after three hours from giving amitraz administration in groups one, two, three, and four. Group five received 500mg/kg and had organ collection after 5 hours of administration, group six received the same as last dose and had organ collection after 24 hours of administration. The results revealed that amitraz produced severe histopathological effects on the liver and kidney tissues compared to the control group. The changes were obvious in rats treated with high dose groups. Severe and significant histopathological changes were evident in the hepatocyte and kidney tissues in the group six of the treated rats compare to the group 5 and 4 of rats. While the lowest given dose group showed normal tissues, indicating that amitraz requires a larger quantity to produce the histopathological action. The study concluded that amitraz has hepatotoxic and nephrotoxic effects when overdose is used.

Keywords

Main Subjects

Pharmacology and toxicology

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