Mustafa, F., Talat Naoman, U. (2021). Comparative Study of Using Four Types of Avian Egg Yolk for Epididymal Sperms Chilled Storage in Awassi Rams (Ovis aries). Journal of Applied Veterinary Sciences, 6(4), 46-50. doi: 10.21608/javs.2021.88532.1096
Faten Fadhel Mustafa; Uday Talat Naoman. "Comparative Study of Using Four Types of Avian Egg Yolk for Epididymal Sperms Chilled Storage in Awassi Rams (Ovis aries)". Journal of Applied Veterinary Sciences, 6, 4, 2021, 46-50. doi: 10.21608/javs.2021.88532.1096
Mustafa, F., Talat Naoman, U. (2021). 'Comparative Study of Using Four Types of Avian Egg Yolk for Epididymal Sperms Chilled Storage in Awassi Rams (Ovis aries)', Journal of Applied Veterinary Sciences, 6(4), pp. 46-50. doi: 10.21608/javs.2021.88532.1096
Mustafa, F., Talat Naoman, U. Comparative Study of Using Four Types of Avian Egg Yolk for Epididymal Sperms Chilled Storage in Awassi Rams (Ovis aries). Journal of Applied Veterinary Sciences, 2021; 6(4): 46-50. doi: 10.21608/javs.2021.88532.1096
Comparative Study of Using Four Types of Avian Egg Yolk for Epididymal Sperms Chilled Storage in Awassi Rams (Ovis aries)
Department of Surgery and Theriogenology, College of Veterinary Medicine, University of Mosul, Iraq.
Receive Date: 31 July 2021,
Revise Date: 20 August 2021,
Accept Date: 04 September 2021
Abstract
The objective of current study was to use four types of egg yolks for ram epididymal sperms The objective of current experiment was to study the effect of different avian egg yolks (EYs) on rams epididymal sperms stored at 4 ºC. Sixteen pairs of testes (n=32) were mature Awassi rams of about 1-4 years, slaughtered at a Al-Sadoon abattoir/ Mosul city were collected. The spermatozoa were collected from tail of epididymis by cutting and squeezing, after that a total of 0.5 ml of spermatozoa volume was diluted by using sodium citrate 2.9% and the volume completed to 1 ml, then the sample divided into four aliquots (0.25 ml for each one). The extender consisted of egg yolk (EY) 10% of four avian types (chicken, quail, duck, turkey), sodium citrate 2.9%, fructose 2.4gm, penicillin, and streptomycin (100.000 IU and 100 mg, respectively) in 100 distal water. Sperms individual motility, live sperm percentage, sperms abnormalities checked at 0, 72, 144 hours of storage. Results of the present study revealed that quail egg yolk improved sperms, individual motility, live sperm percentage with longer life span and reduce sperms abnormalities, followed by Turkey, chicken and duck EYs respectively, Individual motility of the spermatozoa after 144 h of storage in quail EY extender were (36.00%± 1.20), which higher than turkey EY (32.30%± 1.30). However, these results higher and significantly (P˂ 0.05) when compare with chicken EY extender (24.60%± 2.30) and duck EY extender (19.00%± 1.50). Similar results of quail and turkey EYs were manifested in the percentage of live spermatozoa, which were (38.00%± 1.20), (33.30%± 1.30), (24.00%± 2.30), and (19.90%± 1.50), respectively. The duck EY was significantly lower (P˂ 0.05) when comparing between the four types of egg yolks after storage for 144 h. In conclusion: we advise potentially to use of a quail or turkey EYs extender for the chilled storage for ram epididymal sperms.
ABU, AH, KISANI, A. I and AHMEN, T. 2016. Evaluation of cauda epididymides of Red Sokoto bucks. Veterinary world. (9): 1440-1444. DOI: 10.14202/vetworld.2016.1440-1444.
ACHI, J.N., BARJE, P.P., REKWOT, P .I., ACHI, N. P and ALPHONSUS, C. 2017. Effect of Four Different Avian Egg Yolks Tri- Sodium Citrate Extender On Storage Time Of Frisian X Bunaji Semen Characteristics. Journal of Animal Production Research. 29 (1):31-40.
AHMADI, E., NAZARI, H., DAVOODIAN, N and KADIVAR, A . 2020. Feasibility of long-term ovine epididymal spermatozoa preservation in a simple extender containing egg yolk. Journal of Livestock Science and Technologies, 8(1): 59-67. DOI: 10.22103/jlst.2020.14524.1284.
Al-ANY, A.A.T., Al-TAMIMY, S.M.A and Al-MASHADANY, W.A.S. 2016. The Protective Effect of Egg Yolk From Different Avian Species of Awassi Ram Semen During Diluted in Tris-Egg Yolk Extender and Stored At 5°C. Tikrit Journal of Agriculture Science, 16 (2): 116-120.
ALI, A.B.T., BOMBOI, G., FLORIS, B. 2013. Replacing chicken yolk with yolks from other sources in ram semen diluents and their effects on fertility in vitro. Small Ruminant Research, 113:405–410. https://doi.org/10.1016/j.smallrumres.2013.01.017.
CHOI, SH, SONG, KT and OH, HR 2001. Cholesterol contents and fatty acid composition of a chucker, pheasant, guinea fowl and quail egg yolk. Asian-Australian Journal of Animal. Science, 14: 831-836.
DURAND, M.P., RAMOS, L.A., ESPINOZA, R. R., DELGADO, R. A., MESTAS, R.R., GUZMAN, J.A., GUERRA, U.P . 2020. Cryoprotective effect of the egg yolk of different species on the sperm quality of rams under conditions of the Peruvian Altiplano. Supernova, 10(1): 46-51. DOI. 10.18548/aspe/0008.07.
El-SHARAWY, M. E., El-SEIFY, E. M., HUSSIEN, A.M., El-SHERBIENYM, and El-SHAMAA, I. S. 2015. Effect of Duck Egg Yolk on Cryopreservation and Fertility of Egyptian Buffalo Bull Semen. International Journal of Agriculture Innovations and Research, 3(4): 2319-1473.
FERDINAND, N., THOMAS, T.T., AUGUSTAVE, K., HENERY, D.F., FERNAND, T., ETIENNE, P.T. 2012. Effects of buck age, storage duration, storage temperature and diluent on fresh west African dwarf buck semen. Journal 0F Reproduction Infertility, 3(3): 58-66. DOI: 10.5829/idosi.jri.2012.3.3.66216.
HERMANSSON, U and AXNER, E. 2007. Epididymal and ejaculated cat spermatozoa are resistant to cold shock, but egg yolk promotes sperm longevity during cold storage. Theriogenology, (67): 1239-1248. https://doi.org/10.1016/j.theriogenology.2007.01.008
KULAKSIZ, R., CEBI, C., AKACY, E and DASKIN, A. 2010. The protective effect of egg yolk from different avian species during the cryopreservation of Karayaka ram semen. Small Ruminant Research, 88: 12 - 15. https://doi.org/10.1016/j.smallrumres.2009.11.014.
MAHDI, S.W., Al-SHAMARY, M.S., JAAFIR, Z.S. 2019. Role of spermatozoa in PH stability of caudal epididymis environment. Iraqi Journal of Veterinary Science; 33(1): 111-116. 10.33899/ijvs.2019.125511.1033.
NAOMAN, U. T. 2005. Study The effect of month and treatment with HCG hormone on quality and quantity of the semen before and After hemicastration in Iraqi Bucks - Nenava Governorate -MSc thesis. College of Veterinary medicine. University of Mosul. Mosul. Iraq.
RAWASH, Z., LIEL A., ANWER, A and El-BADRY, D. A. 2020. Influence of Combination of Some Permeable Cryprotectants with Chicken and Duck Egg Yolks on Freezability and DNA Integrity of Buffalo Spermatozoa. Journal of Applied Veterinary Sciences, 5 (3): 49 – 56. DOI: 10.21608/JAVS.2020.98371.
SAIEED, A.Y., MAHDI, Z.A., IBRAHIM, A.A. 2018. Effect of addition from egg yolk of different avian to Tris extender on freezing semen traits of Awassi rams. Iraqi Journal of Agriculture Science, 49(1): 663-669. DOI: https://doi.org/10.36103/ijas.v49i4.76.
SUNDAY, A.A., TAIWO, O. J., OLUFEMI, A., ABEL ADEYEMI, A. 2018. Effects of Egg Yolks from Different Avian Species on Boar Sperm Motility and Livability. Journal of Biology, Agriculture and Healthcare. 8(6):24-28.
SWELUM, A.A., SAADELDIN, I. M., AlANAZIA, M.B., AWADHA, H.B., AFIFIDI, M., Al OWAIMER, A. N. 2018. Effects of adding egg yolks of different avian species to Tris glycerol extender on the post-thawing quality of buck semen. Animal Reproduction Science, 195: 345–354. https://doi.org/10.1016/j.anireprosci.2018.06.016.
TRIMECHE, A., ANTON, M., RENARD, P., GANDEMER, G and TAITURIER, D. 1997. Quail egg yolk: a novel cryprotectant for the freeze preservation of Poitou jackass sperm. Cryobiology, 34: 385-393. https://doi.org/10.1006/cryo.1997.2009.
TUNNER, Z., CANISSO I., PODICO G., GARRET E., STEWART, J., HENLEY, P. 2020. Cryopreservation of epididymal and Electro-ejaculated bull semen using liposome and egg yolk-based extender. Clinical Theriogenology, 12(1): 29-38.
UYSAL, O., BUCAK. MN 2007. Effects of oxidized glutathione, bovine serum albumin, cysteine and lycopene on the quality of frozen-thawed ram semen. Acta Veterinary Brno, 76: 383-90. Doi:10.2754/avb200776030383.
WAHEED, S., AHMAD, N., RAHMAN, N., RAHMAN, H., YOUNIS, M., IQBAL, S. 2012. Evaluation of duck egg yolk for the cryopreservation of Nili-Ravi buffalo bull semen. Animal Reproduction Science. 131: 95–99. Doi:10.1016/j.anireprosci.2012.02.011.
View on SCiNiTO
Top