A. Radwan, A., Mikhael, C. (2020). Comparative Evaluation On The Efficacy Of Embryonated Chicken Egg Adapted And Tissue Culture Pigeon Pox Vaccines Against The Local Virulent Strain. Journal of Applied Veterinary Sciences, 5(2), 87-93. doi: 10.21608/javs.2020.85605
Amina A. Radwan; Christine A. Mikhael. "Comparative Evaluation On The Efficacy Of Embryonated Chicken Egg Adapted And Tissue Culture Pigeon Pox Vaccines Against The Local Virulent Strain". Journal of Applied Veterinary Sciences, 5, 2, 2020, 87-93. doi: 10.21608/javs.2020.85605
A. Radwan, A., Mikhael, C. (2020). 'Comparative Evaluation On The Efficacy Of Embryonated Chicken Egg Adapted And Tissue Culture Pigeon Pox Vaccines Against The Local Virulent Strain', Journal of Applied Veterinary Sciences, 5(2), pp. 87-93. doi: 10.21608/javs.2020.85605
A. Radwan, A., Mikhael, C. Comparative Evaluation On The Efficacy Of Embryonated Chicken Egg Adapted And Tissue Culture Pigeon Pox Vaccines Against The Local Virulent Strain. Journal of Applied Veterinary Sciences, 2020; 5(2): 87-93. doi: 10.21608/javs.2020.85605
Comparative Evaluation On The Efficacy Of Embryonated Chicken Egg Adapted And Tissue Culture Pigeon Pox Vaccines Against The Local Virulent Strain
Central Laboratory for Evaluation of Veterinary Biologics (CLEVB), Abbasia, Cairo, Egypt, P.O.Box131
Receive Date: 18 March 2020,
Revise Date: 17 April 2020,
Accept Date: 21 April 2020
Abstract
A total of 150 pigeons of 45 days old was used and divided into three groups; the first one was vaccinated with tissue culture adapted pigeon pox vaccine (TCAPPV), and the second was vaccinated with egg adapted pigeon pox vaccine (EAPPV) and the third as a non-vaccinated control group. Birds were observed for ten days post-vaccination (DPV) for the presence of takes. Cellular immunity was detected by lymphocyte proliferation assay on the whole blood for 21 DPV, and serum samples were collected weekly. The level of induced antibodies was detected by the neutralization test for six months post-vaccination. Twenty pigeons of each group were challenged by virulent pp virus at 28th DPV Takes were recognized at the site of vaccination at 4thDPV and increased to the maximum at7th DPV to reach 90% for TCAPPV and 98% for EAPPV. The peak of the cellular immunity by lymphocyte proliferation assay was at the 12thDPV when TCAPPV recorded 1.534 and EAPPV 2.037. Protection was 90% for TCAPPV and 100% for EAPPV. The peak of neutralizing index (NI) at 35thD.P.V for both vaccinated groups; It was 2.75 for TCAPPV and 3.25 for EAPPV. Both vaccines are still potent to the end of examination at the 6thmonth when NI was 1.5 for TCAPPV and 2.0 for EAPPV. This result shows that both eggs adapted PP and tissue culture PP vaccines are efficient in the protection of pigeons in Egypt despite the egg adapted vaccine is more preferable.
ABDALLAH F.M. AND HASSANIN O. 2013. Detection and molecular characterization of avipoxviruses isolated from different avian species in Egypt. Virus Genes Vol. 46, No: 1. P: 63-70.
ANDRAW, K. 2012. Virus taxonomy: ninth report of the international committee on the taxonomy of viruses. The international committee on taxonomy of international union of microbiological societies.PP.298.
BOSSINGER, T.R.; WINTERFIELD, R. W.; FELDMAN, D.S. AND DHILLON, Α. S. 1982. Psittacine pox virus: virus isolation and identification, transmission and cross challenge studies in parrots and chickens. Avian Dis, 26: 437 – 444.
BRANSON, W.R. AND KIP, C. 1995. Avian Viruses: Function and Control, 10: 285-311.
BULLER , R.M. AND PALUMBO, G.J.1991. Poxvirus pathogenesis, Microbiol.Rev.55:80-122.
CAROL, D.E. AND MARINESCU, M. 1971. Antigenic relationship between fowlpox, pigeon pox and turkey pox viruses determined by serum neutralization test. Lucrari Stuntifice Institutul Agronomic N. Balcescu , Seria C., 14: 217-227.
CHARLES, R., CATPENTER, A.B.; HENRY, R. AND BOSE, J.R. 1978. Suppression of the mitogen-stimulated blastogenic response during reticuloendotheliosis virus-induced tumorigenesis. J. Immunol., 120(4): 1313-1320.
CODE OF FEDERAL REGULATIONS (CFR) USA 2012. Published by the Office of Federal Register National Archives Records Service, Animals 331-13; CFR
COTTRAL, G. E. 1978. Poxviruses.In manual of Standardized Methods for Veterinary Microbiology, ed. G.E.Cottral.Cornell University Press, PP 273- 291.
DASGUPTA, A., HAMMARLUND, E., SLIFKA, M.K. AND FRÜH, K. 2007. Cowpox Virus Evades CTL Recognition and Inhibits the Intracellular Transport of MHC Class I Molecules. J. Immun. 178, 1654.-1660.
FRANK, J.; MARK, P.; DENNIS, A. AND TREVOR, F. 2001B. Poxviridae. Poultry Diseases. 5th Edition, chapter 22: 173 - 187.
FRANK, J.; MARK, P.; DENNIS, A. AND TREVOR, F. 2001A. Vaccines and vaccination. Poultry Diseases. 5th Edition chapter 4: 55 - 70.
GOTTSTEIN, Ζ.; CIGLAR G. I.; HORVATEK, D.; PRUKNER-RADOVCIC, E.; CAJAVEC, S. AND MAZIJA, H.2004. Safety and immunogenicity of pigeon pox virus vaccine applied to newly hatched chickens by means of nebulization, In Book of Αbstracts XXII World Poultry Congress, Istanbul, Turkey.
HELMY N. AHMED, ABDEL H. EL SABBAGH AND G. ZAHRAN 1967. Studies on pigeon pox vaccine in the U.A.R. Preparation and Application of Desiccated chorioallantoic membrane vaccine. Journal of the Egyptian Veterinary Medical Association.Volxxvii:85-90.
HEMANTH, I., AMARAVATHI, P., SASIDHAR, B. N., ANAND K. A AND SAILAJA, N. 2014. An outbreak of cutaneous form of avian pox in pigeons (Columbia Livia) International Journal of Science, Environment and Technology, Vol. 3, No 4, 1484 – 1488.
JOSHI, R. K. AND NAMITAL, J. 2011. Growth and cytopathogenicity of a field isolate of fowlpox virus in BGM-70 cell line. Ind. J. Comp. Microb., Immun. Infec. Dis., 32, 19-21.
KAFAFY, M.H., ABOUL-SOUD, E.A., OLFAT, E. NAKHLA, CHRISTIN. A. MICHAEL AND NERMIN M. EL-SAID 2018. Preliminary studies on preparation and evaluation of a local isolate tissue culture propagated pigeon pox vaccine. Vet. Med. Journal. Vol. (64): 39-50.
LUCY, F.L. 1984. Proliferative response of chicken B and T lymphocytes to mitogens, Chemical regulation of immunity in veterinary medicine, 15:44-52.
MICHAEL, A., MOUNIRA, I. NASSAR AND EL-SABBAGH, A.H. 1986. Combined vaccination with pigeon and fowlpox vaccines. Assiut Vet.Med.J .16: 365-379.
MICHAEL, A.L. 1981. Further studies on fowlpox vaccines and their efficacy in vaccinating birds. PhD Thesis Fac. Vet.Med. (Poultry Diseases) Cairo University.
MISHRA, S.S. AND MALLICK, B.B. 1994. Structural proteins of fowlpox virus vaccine strain and field isolates. Indian Journal of Experimental Biology. Vol. 32: 826-831.
NAMAA, A. MOHAMMED. 1998. Study on trial for preparation of fowl pox vaccine locally on embryonated specific pathogen-free eggs. M.V.Sc. Thesis, Faculty of Veterinary Medicine, Cairo University.
OLFAT. E. NAKHALA, MERVAT. M. ALI, MICHEAL. A. AND SOAD. M. SOLIMAN 2005. Adaptation and propagation of the Egyptian strain of pigeon pox on different types of cell cultures. J Vet Med Assoc. Vol. (65) pages 191-202.
PCT 2005. Immortalized avian cell lines for virus production. International application published under the patent cooperation treaty. World Intellectual Organization, International Bureau. International publication ( no 0.042728 A2.)
REED, L.J. AND MUENCH, H. 1938. A simple method of estimating fifty percent endpoints. Am. J. Hyg,27:493-497.
SAINOVA, I.V., KRIL, A.I., SIMEONOV, K.B., POPOVA, T.P., IVANOV I.G. 2005. Investigation of the morphology of cell clones, derived from the mammalian EBTr cell line and their susceptibility to vaccine avian poxvirus strains F.K. and Dessau. J. Virol. Meth, 124, 37.
SCHAT K.A. AND PURCHASE H.G. 2008. Cell culture methods. In: Laboratory Manual for the Isolation, Identification and Characterization of AVIAN Pathogens. Ch. 43. Athens, GA: American Association of Avian Pathologists, P 195-203.
SOAD, M. S.: ABD EL-RAZIK, B. A: AMAL, F: AMIRA, A.E. AND MICHEAL, A. 2007. Antigenic comparative studies between vaccinal and virulent strains of pigeon pox viruses Zag. Vet. J. Vol (35), No. (1) PP 8-13.
SOAD, M. SOLIMAN 1986. Immunological and virological studies on fowlpox vaccine. Ph.D.Thesis, Faculty of Veterinary Medicine, Cairo University.
SUMAYA K. M. K. 2005. Pathogenic antigenic and serologic relationship between fowl pox, pigeon pox and canary poxviruses. M.V.Sc., U.K.
TRIPATHY, D.N. AND REED, W.M.1997. Pox. Diseases of Poultry, Chapter 24: 643-659
TRIPATHY, D.N. AND REED, W. M. 2008. Avian pox in Diseases of Poultry, 12th Ed., ed. by Y.M.
WANG, J.; MEERS, J.; SPRADBROW, P. B. AND ROBINSON, W. F. 2006. Evaluation of immune effects of fowl pox vaccine strains and field isolates. Vet. Microbial. 116: 106 – 119.
WELI SC., NILSSEN O., TRAAVIK, T. 2005. Avipox virus multiplication in a mammalian cell line. Virus Res, 109, 39-49.