Toxicopathological Impacts Of Chlorpyrifos On Sperm Qualities And Testicular Tissue Alterations And Their Modulation With Vitamin E And Zinc In Male Albino Rats

Document Type : Original Article

Authors

1 Dept. of Reproductive Pathology, Animal Reproduction Research Institute, Agricultural Research Center, Egypt

2 Department of Reproductive Pathology, Animal Reproduction Research Institute, Agricultural Research Center, Egypt

3 Artificial Insemination and Embryo Transfer Dept, Animal Reproduction Research Institute, Agriculture Research Center, Egypt

Abstract

This study was carried out on 30 mature male albino rats (2 months old, weighed about 130 g) in 3 groups of ten rats for each one in ARC. Group I, kept as control. Group II, daily intubated with CPF, at a dose level of 10.6 mg/kg BW in corn oil. Group III, daily intubated with CPF at a dose level of 10.6 mg/kg BW, zinc at a dose level of 227 mg/kg BW and vitamin E at a dose level of 75 mg/kg BW for 50 days equivalent to one spermatogenic cycle in rats. At the end of the experiment, the rats were sacrificed to take blood, semen and tissue specimens. Histopathologically, the testes and epididymis showed moderate degeneration and thickening of the tubular membrane with interstitial oedema in the CPF intoxicated group. These changes were ameliorated in the rats of group III. The semen analysis is aggressively badly affected in the CPF intoxicated group in comparison with the control group and improved in rats of group III. Testosterone hormone levels were assayed with ELISA technique as 3.871±0.31 ng/ml, 1.112±0.82 ng/ml, and 2.503±0.25 ng/ml in control, CPF, CPF plus vitamin E and Zn groups, respectively. Moreover, the DNA integrity of spermatozoa in the CPF group was severely affected in comparison with either the control group and group III.

Keywords

Main Subjects

References

AKAN J.C.; JAFIYA L.; MOHAMMED Z. AND ABDULRAHMAN F. 2013. Organophosphorus pesticide residues in vegetables and soil samples from alau dam and going along agricultural areas, Borno State, Nigeria. International Journal of Environmental Monitoring and Analysis, 1, 2; 58-64.
AKHGARI M.; MOHAMMED A.; ABBAS K.; RUHOLLAH H. AND Omid S. 2003. Biochemical evidence for free radical-induced lipid peroxidation as a mechanism for subchronic toxicity of malathion in blood and liver of rats. Human and Experimental Toxicology, 22, 4; 205-211.
Boe-Hansen G.B.; Fedder J.; Ersboll A.K. and Christensen P. 2006. The sperm chromatin structure assay as a diagnostic tool in the human fertility clinic. Human Reproduction, 21, 6; 1576-1582.
Bray T.M. and Bettger W.J. 1990. The physiological role of zinc as an antioxidant. Free Radical Biology and Medicine, 8, 3; 281:291.
Costat Computer Program Copyright 1986. Version 3.03 copyright Cottort Software.
Debnath D. and Mandal T. 2000. Study of quinalphos (an environmental oestrogenic insecticide) formulation (Ekalux 25 E.C.) ‐induced damage of the testicular tissues and antioxidant defense systems in Sprague-Dawley albino rats. J. Appl. Toxicol., 20; 197-204.

Goel A.; Dani V. and Dhawan D.K. 2007.Zinc mediates normalization of hepatic drug-metabolizing enzymes in chlorpyrifos-induced toxicity. Toxicology, 169, 1; 26-33.

Goel A.; Dani V. and Dhawan D.K. 2015. Protective effects of zinc and vitamin E on lipid peroxidation, antioxidant enzymes and lung histoarchitecture in chlorpyrifos-induced toxicity. Chemico-Biol. Inter., 156; 131-134.
Gupta R.C. 2018.Organophosphates (Toxicity). Merk Manual Veterinary Manual.
Hughes C.M.; Lewis S.E.; McKelvey-Martin V. and Thompson W.A. 1996. comparison of baseline and induced DNA damage in human spermatozoa from fertile and infertile men, using a modified comet assay. Mol. Hum. Reprod., 2: 613-619.;
Khalifa K.;  Khalil A.; Barakat H. and  Hassan M. 2011. Protective role of wheat germ and grape seed oils in chlorpyrifos-induced oxidative stress, biochemical and histological alterations in liver of rats”. Australian Journal of Basic and Applied Sciences, 5; 54-66.
Kovgac G.; Baertko P.; Vrzgula L.; Reichel P.; Tiar F.; Mozhi J. and Seidel H. 1995. Application of clinoptilolite during organophosphate intoxication in sheep, lnternat. Comm. Natural Zeolites. Brockport, New York. Natural Zeolites 93, pp: 459-466.

Li Q.; Kobayashi M. and Kawada T. 2009. Chlorpyrifos induces apoptosis and fibrosis in rat lung. Toxicology, 255; 53-57.

Liu X.Y.; Jin T.Y.; Nordberg G.F.; Rannear S.; Sojostrom M. and Zhou Y.A. 1998. Multivariate study of protectiveeffects of Zn and Cu against nephrotoxicity induced by cadmium metallothionein in rats. Toxicology and Applied Pharmacology, 151, 2; 276:282.
Mansour S.A. and Mossa A. 2009. Lipid peroxidation and oxidative stress in rat erythrocytes induced by chlorpyrifos and the protective effect of zinc. Pesticide Biochemistry and Physiology, 93, 1; 34-39.
Mansour S.A. and Mossa A.H. 2010. Adverse effects of lactational exposure to chlorpyrifos in suckling rats. Toxicol., 29, 2, 77-92.
Mansour S.A.; Heikel T.; Refaie A. and Gamet-Payrstre L. 2011. Antihepatotoxic activity of fennel (Foeniculum vulgare Mill.) essential oil against chlorpyrifos-induced liver injury in rats. Global Journal of Environmental Science and Technology, 1; 10-11.
Mansour S.A. and GAMET-Payrstre L. 2016. Ameliorative effect of vitamin E to mouse dams and their pups following exposure of mothers to chlorpyrifos during gestation and lactation periods. Toxicology and Industrial Health, 32, 7; 1179-1196.
Mansour S.A.; ABBASY M.A. AND SHALDAM H.A. 2017. Hepato-Renal Toxicity Induced by Chlorpyrifos, Diazinon and their Mixture to Male Rats with Special Concern to the Effect of Zinc Supplementation. Journal of Toxicology and Pharmacology, 1; 1-15.
Maroni M.; Colosio C.; Ferioli A. and Fait A. 2000. Biological monitoring of pesticide exposure: a review. Introduction. Toxicology, 143, 1; 1-118.
Olayemi F.O. 2007. Evaluation of the reproductive and toxic effects of Cnestis ferruginea de Candolle) root extract in male rats. Ph. D Thesis. Dept. of Physiology, University of Ibadan, 26: 263.
Olorunshola K.V.; Achie L.N. and Akpomiemie M.L. 2011.Ascorbic acid ameliorates the toxic effects of chlorpyrifos on testicular functions of albino rats. British Journal of Pharmacology and Toxicology, 2, 5; 262-269.
Peiris D.C. and Dhanushka T. 2017.Low doses of chlorpyrifos interfere with spermatogenesis of rats through reduction of sex hormones. Journal Environmental Science and Pollution Research, 24, 26; 859-867.
Powell S.R. 2000.The antioxidantproperties of zinc. Journal of Nutrition, 130, 5; 1447-1454.
Rocha J.S.; Bonkowski M.S. and Franca L.R. 2007. Mild calorie restriction does not affect testosterone levels and testicular gene expression in mutant mice. Exp Biol Med (May-Wood) 232; 1050-1063.
Sai L.; Li X.; Liu Y.; Guo Q.; Xie L.; Yu G.; Bo C.; Zhang Z. and Li L. 2014. Effects of chlorpyrifos on reproductive toxicology of male rats. Environmental Toxicology, 29, 9; 1083-1088.
Sinha N.; Narayan R.; Shanker R. and Saxena D.K. 1995. Endosulfan induced biochemical changes in the testis of rats. Veterinary Hum Toxicol, 37; 547-551.
Smith J.T. and Mayer D.T. 1955.Evaluation of sperm concentration by the hemocytometer method comparison of four counting fluids. Fert. Steril., 6; 271:275.
ThomsonW.T. 1992. Agricultural chemicals. Book 1, Insecticides, English, Book, Illustrated edition: acaricides and ovicides.
Viswanath G.; Chatterjee S.; Dabral S.; Nanguneri S.R.; Divya G. and Roy P. 2010. Anti-androgenic endocrine disrupting activities of chlorpyrifos and piperophos. The Journal of Steroid Biochemistry and Molecular Biology, 120, 1; 22-29.
Wells M.E. and Awa O.A. 1970.New technique for assessing acrosomal characteristics of spermatozoa. J. Dairy Sci., 53, 2; 227:232.
Woston S. and Martin R. 1972. A comparison of change in acrosomes of deep-frozen ram and bull spermatozoa. J. Reprod. Fert., 28, 1; 99-101.
 

Files

History

  • Receive Date: 24 March 2020
  • Revise Date: 04 April 2020
  • Accept Date: 07 April 2020

Share

How to cite

Statistics