Preparation And Characterization Of Natural Nano Hydroxyapatite From Eggshell And Seashell And Its Effect On Bone Healing

Alhussary, Banan N.; A.Taqa, Ghada; Taqa, Amer Abd Alrhman

doi:10.21608/javs.2020.85567

Preparation And Characterization Of Natural Nano Hydroxyapatite From Eggshell And Seashell And Its Effect On Bone Healing

Document Type : Original Article

Authors

¹ Department of Dental Basic Sciences , College of Dentistry. University of Mosul , Mosul , Iraq.

² Professor in veterinary pharmacology and toxicology, College of Dentistry , Mosul university , Iraq

10.21608/javs.2020.85567

Abstract

The Objectives of the present study were undertaken to prepared hydroxyapatite powder from eggshell and seashell and convert them in Nano size. Hydroxyapatite (HA) is a biomaterial with osteoconductive properties and is prepared by the precipitation method. The properties of HA can be enhanced when the material is synthesized in the Nanoscale range to use in the mandibular bone of the rabbit to examine the influence of (HA) on bone healing.The eggshell and seashell were cleaned, and the phosphoric acid was added. The product inserts in the oven then calcined at 700˚C for 1 hour in the muffled furnace to evaporate CO₂ and to get the white crystalline powder, which indicated the presence of HA, this powder was converting to nanoparticle by attraction method. The product was applied in the groove of the rabbit's mandibular bone after surgical procedures to show the bone healing in histological examination. The results of IR Spectroscopy for the eggshell and seashell hydroxyapatite powder showed that the two compounds showed the same IR spectrum and the location of bands is approximately the same in the two compounds compared with the commercial hydroxyapatite powder by using FT-IR spectroscopy after dried at 100˚C. Histologically show that eggshell and seashell hydroxyapatite powder accelerated the bone healing in the mandibular bone of the rabbits. It can be concluded that hydroxyapatite nanoparticles can successfully be produced by chemical precipitation techniques from eggshells and seashells with a phosphoric acid solution. Eggshell and seashell hydroxyapatite powder was used successfully in bone healing

Keywords

Main Subjects

Nanotechnology

References

ANCHANA D and PRIYA P. 2016. Synthesis and Application of Hydroxyapatite Bioceramics from Different Marine Sources. Journal of Research in Environmental and Earth Science, 2(11): 7-15.

CACCHIOLI, A., SPAGGIARI, B., RAVANETTI, F., MARTINI, F. M., BORGHETTI, P. & GABBI, C. 2006. The critical-sized bone defect: a morphological study of bone healing. Ann. Fac. Medic. Vet. di Parma, 26, 97-110.

CALCIOLARI, E., DONOS, N., & MARDAS, N. 2017. Osteoporotic animal models of bone healing: advantages and pitfalls. Journal of Investigative Surgery, 30(5), 342-350.

CLAUDIO L. DE CASTRO, BRAIN S. 2005. Mitchell Department of Chemical Engineering, Tulane University, New Orleans, Louisiana, USA, ( chapter one).

DADHICH, P., DAS, B., Pal, P., SRIVAS, P. K., DUTTA, J., RAY, S., & DHARA, S. 2016. A simple approach for an eggshell-based 3D-printed osteoinductive multiphasic calcium phosphate scaffold. ACS applied materials & interfaces, 8(19), 11910-11924.

DOROZHKIN, S. V. 2009. Nano dimensional and Nanocrystalline apatite and other calcium orthophosphates in biomedical engineering, biology, and medicine. Materials, 2(4), 1975-2045.

FARA, A.; KHALIS, A. N.; BIN YAHYA, M. A., and ABDULLA, H. Z. 2015. Preparation and Characterization of Biological Hydroxyapatite (HAp) Obtained from Tilapia Fish Bone. Advanced Materials Research, 1087(1):152-156.

HIRATSUKA, T., UEZONO, M., TAKAKUDA, K., KIKUCHI, M., OSHIMA, S., SATO, T., ... & MORIAMA, K. 2020. Enhanced bone formation onto the bone surface using a hydroxyapatite/collagen bone-like Nanocomposite. Journal of Biomedical Materials Research Part B: Applied Biomaterials, 108(2), 391-398.

KHANDELWA, H. and PRAKASH S. 2016. Synthesis and Characterization of Hydroxyapatite Powder by Eggshell. Journal of Minerals and Materials Characterization and Engineering, 4(02): 119.

MAHA A.Al-MURAD, MA’AN M. NAYIF, AMER A. TAQA, 2019. The Effect of incorporation Hydroxyapatite nano-particles on Shear Bond Strength of Self -Adhesive Resin Cement, acceptance for publication journal of oral research.

NANCI, A. 2017. Ten Cate's Oral Histology-E-Book: Development, Structure, and Function. Elsevier Health Sciences.

ORMAN, Z., YUSEL, S., SAHIN, Y. M., GUNDUZ, O., & OKTAR, F. N. 2019. Bioactivity of Hydroxyapatite Produced from Sea Snail Turritella Terebra. Acta Physica Polonica, A., 135(5).

PARK, J. B. & BRONZINO, J. D. 2003. Biomaterials principles and applications. (Eds.), Boca Raton, London, New York, Washington DC, CRC Press.

RIPAMONTI, U.; CROOKS, J.; KHOLI, L. and RODEN, L. 2009. The induction of bone formation by coral-derived calcium carbonate/hydroxyapatite constructs. Biomaterials, 30(7): 1428-1439.

SALAMA, R., KHASHABA, M., & El ROUBY, D. 2019. Histomorphometric evaluation of a Nano-sized eggshell-containing supplement as a natural alloplastic: An animal study. The Saudi dental journal, 31(3), 375-381.

SHAPOVALOVA, Y., LYTKINA, D., RASSKAZOVA, L., ZHUK, I., GUDIMA, A., FILIMOSHKIN, A., ... & KZHYSHKOWSKA, J. 2016. Preparation of biocompatible composites based on poly-L-lactide/hydroxyapatite and investigation of their anti-inflammatory activity. In Key Engineering Materials (Vol. 683, pp. 475-480).

SZCZES, A., HOLYSZ, L., & CHIBOWSKI, E. 2017. Synthesis of hydroxyapatite for biomedical applications. Advances in colloid and interface science, 249, 321-330.

TANGBORIBOON, N.; KUNANURUKASAPONG, R. and SIRIVAT, A. 2012. Preparation and properties of calcium oxide from eggshells via calcination. Materials Science-Poland, 30(4): 313-322.

TRON, A. C. 2016. Decellularized whole organs as vascularized bioscaffolds for bone tissue engineering (Doctoral dissertation, Technische Universität Münchn.