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Ibrahim, E., El Helw, H., Shafik, N., Mekhail, M. (2017). Conjugation Of Foot And Mouth Disease IgY In Chicken Egg Yolk With Horse Radish Peroxidase For Typing Of Foot And Mouth Disease Virus. Journal of Applied Veterinary Sciences, 2(1), 35-42. doi: 10.21608/javs.2017.65605
Ehab E. Ibrahim; Hamed A.H. El Helw; Nermen Guda Shafik; Manal Awad Mekhail. "Conjugation Of Foot And Mouth Disease IgY In Chicken Egg Yolk With Horse Radish Peroxidase For Typing Of Foot And Mouth Disease Virus". Journal of Applied Veterinary Sciences, 2, 1, 2017, 35-42. doi: 10.21608/javs.2017.65605
Ibrahim, E., El Helw, H., Shafik, N., Mekhail, M. (2017). 'Conjugation Of Foot And Mouth Disease IgY In Chicken Egg Yolk With Horse Radish Peroxidase For Typing Of Foot And Mouth Disease Virus', Journal of Applied Veterinary Sciences, 2(1), pp. 35-42. doi: 10.21608/javs.2017.65605
Ibrahim, E., El Helw, H., Shafik, N., Mekhail, M. Conjugation Of Foot And Mouth Disease IgY In Chicken Egg Yolk With Horse Radish Peroxidase For Typing Of Foot And Mouth Disease Virus. Journal of Applied Veterinary Sciences, 2017; 2(1): 35-42. doi: 10.21608/javs.2017.65605

Conjugation Of Foot And Mouth Disease IgY In Chicken Egg Yolk With Horse Radish Peroxidase For Typing Of Foot And Mouth Disease Virus

Article 5, Volume 2, Issue 1, October 2017, Page 35-42  XML PDF (314.06 K)
Document Type: Original Article
DOI: 10.21608/javs.2017.65605
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Authors
Ehab E. Ibrahim email orcid 1; Hamed A.H. El Helw2; Nermen Guda Shafik3; Manal Awad Mekhail4
1Department of Foot and Mouth Disease, Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo, Egypt
2Veterinary Serum and Vaccine Research Institute, svri@idsc.gov.eg.
3Central Laboratory for Evaluation of Veterinary Biologics (CLEVB), Egypt
4Veterinary Serum and Vaccine Research Institute, Abbassia, Egypt
Receive Date: 18 August 2019,  Revise Date: 15 September 2017,  Accept Date: 22 September 2019 
Abstract
Through the present work, anti-FMD IgY immunoglobulins for serotypes O; A, and SAT2 were prepared in chicken egg yolk through immunization of laying hens with locally prepared trivalent FMD vaccine. The antibody titres against three serotypes (O; A, and SAT2) measured by SNT were 2.58; 2.8 and 2.6 respectively and where 2.81; 2.81 and 2.8 respectively when measured by ELISA. Evaluation of Anti-FMD IgY conjugated with Horse radish peroxidase for detection of FMD virus in 100 tongue epithelium; 50 OP fluid samples; 20 nasal swabs from naturally infected cattle, and 100 tissue culture infected fluids revealed sensitivity of 85% indicating the success of preparation of anti-FMD immunoglobulins conjugated with horse radish peroxidase as local product available on request saving cost and time.
Keywords
ELISA; FMD; Immunization; PCR; Horse radish peroxidase; Serotypes
Main Subjects
Immunology and Vaccinology
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