Saleh, A., Fathy, N., AbdRabo, M., Helmy, N. (2019). Trials For Preparation And Evaluation Of Inactivated Tissue Culture Newcastle Disease Vaccine From Recent Isolate. Journal of Applied Veterinary Sciences, 4(1), 35-42. doi: 10.21608/javs.2019.62675
Amani A. Saleh; Nada A. Fathy; Mohamed A. AbdRabo; Noha A. Helmy. "Trials For Preparation And Evaluation Of Inactivated Tissue Culture Newcastle Disease Vaccine From Recent Isolate". Journal of Applied Veterinary Sciences, 4, 1, 2019, 35-42. doi: 10.21608/javs.2019.62675
Saleh, A., Fathy, N., AbdRabo, M., Helmy, N. (2019). 'Trials For Preparation And Evaluation Of Inactivated Tissue Culture Newcastle Disease Vaccine From Recent Isolate', Journal of Applied Veterinary Sciences, 4(1), pp. 35-42. doi: 10.21608/javs.2019.62675
Saleh, A., Fathy, N., AbdRabo, M., Helmy, N. Trials For Preparation And Evaluation Of Inactivated Tissue Culture Newcastle Disease Vaccine From Recent Isolate. Journal of Applied Veterinary Sciences, 2019; 4(1): 35-42. doi: 10.21608/javs.2019.62675
Trials For Preparation And Evaluation Of Inactivated Tissue Culture Newcastle Disease Vaccine From Recent Isolate
1Veterinary vaccine and serum research institute (VSVRI), El-Seka El-BaidaSt.Abbasia,Cairo,Egypt.
2Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo,
3Central Laboratory for Evaluation of Veterinary Biologics (CLEVB), El-Seka El-BaidaSt.Abbasia,Cairo,Egypt
Receive Date: 20 March 2019,
Revise Date: 28 November 2019,
Accept Date: 12 April 2019
Abstract
The present study was undertaken to development of BHK-21 cell adapted inactivated vaccine of Newcastle disease virus (NDVgenotype VII) from the field isolate from broiler chicken in Egypt during 2015-2016. The isolatesof El-Giza/2015were classified by sequencing as velogenic NDV genotype VII d contains F protein cleavage site motifs (112RRQKRF117). Such virus was propagated in the BHK-21 cell line. and cell adapted virus was confirmed as NDV by reverse transcription-polymerase chain reaction (RT-PCR) using fusion gene-specific primers and used to develop inactivated vaccine adjuvanted with Montanide IMS 1313.Potency test revealed that Vaccinated chicks with 0.5ml of prepared NDV vaccine exhibited HI antibody titer of 8.6 log2 three weeks post vaccination with the highest titer (10.6 log2/ml) at the 6th-week post vaccination, and 3rd weeks post challenge test. Protective antibodies values were persisting till 12th weeks post vaccination. All chicken groups vaccinated with both prepared inactivated tissue culture vaccine using ISA 1313 and VSVRI inactivated ISA70 adjuvant vaccines were passed challenge test (97.5%,97%,96% protective efficiency to SPF chickens) against the isolated virulent NDV, while the control group could not provide any protective efficiency. The present study indicated that, BHK-21 cell adapted recent isolated NDV inactivated vaccine produced a satisfactory antibodies titre that efficient in control of the disease in Egypt.
AHAMED, T.; HOSSAIN, K. M; BILLAH, M. M; ISLAM, K. M; D. AHASAN M. M. AND ISLAM, M. E. (2004): Adaptation of Newcastle disease virus (NDV) on Vero cell line. Int. J. Poult. Sci., 3(2): 153-156.
Anon.1971.Methods for examining poultry biologics and for identification and quantifying avian pathogens. Newcastle disease, US National Academy of sciences, Wasinghton DC, pp: 66.
CHUN.G. LIU; M. LIU; F. LIU; F. LIU DA; Y. ZHANG AND W.Q. PAN. 2012. Evaluation of several adjuvants in avian influenza vaccine to chickens and ducks Avian Dis., 56 (2012), pp. 128-133.
CODE OF FEDERAL REGULATION. CFR.Title 9 part 82, issues of conditional license for paramyxo virus vaccine type 1.killed virus.
CZERMAK, P.; PÖRTNER, R.;AND BRIX, A. 2009. Special Engineering Aspects. Eibl R, Eibl D, Pörtner R, Catapano G, Czermak P (eds). Cell and Tissue Reaction Engineering: Principles and Practice. Springer-Verlag Berlin. Germany; pp 106-120.
DIEL.D.C; P.J. MILLER; P.C. WOLF; R.M. MICKLEY; A.R. MUSANTE; D.C. EMANUELI; K.J.SHIVELY; K. PEDERSENAND C.L. AFONSO 2012.Characterization of Newcastle disease viruses isolated from cormorant and gull species in the United States in 2010.
Egyptian Protocol 2017. Egyptian Standards Regulation for Evaluation of Veterinary vaccines 3rd Edition.
FENTIE, T.; HEIDARI, A.;AIELLO, R.;KASSA, T.; CAPUA, I.; CATTOLI, G. AND SAHLE, M. 2014. Molecular characterization of Newcastle disease viruses isolated from rural chicken in northwest Ethiopia reveals the circulation of three distinct genotypes in the country. Tropical Animal Health and production, 46, 299–308.
FRINGE R; BOSMAN AM, EBERSOHN K, BISSCHOP S, ABOLNIK C, VENTER E. 2012 Molecular characterization of Newcastle disease virus isolates from different geographical regions in Mozambique in 2005. Onderstepoort J. Vet. Res. 2012; 79: 1-7.
Hassan, M.K.; Afify, M.A.; AND Aly, M.M. 2004.genetic resistance of Egyptain chickens to Newcastle disease. Animal health production, Vol (36) PP: 1-9
HU, Z.; HU, S.; MENG, C.; WANG, X.; ZHU, J.; AND LIU, X. 2011. Generation of a genotype VII Newcastle disease virus vaccine candidate with high yield in embryonated chicken eggs Avian Dis., 55, pp. 391–397.
HUSSEIN H. A., EL- SANOUSI, A.A. AND YOUSIF , A.A. 2005. Sequence analysis of fusion and matrix protein gene of velogenic viscerotropic NDV in egyptain strain SR76. Journal of virol.Vol : 1,38
HUSSEIN H. A.; EMARA, M. M.; AND ROHAIM, M. A.2013. Molecular characterization of Newcastle disease virus genotype VII d in avian influenza H5N1 infected broiler flock in Egypt. Inter. J. Virol., ISSN 1816-4900.
KHAN T.A; REHMANI S.F; AHMED. A; LONE N.A AND KHAN MN.2012. Characterization of Newcastle disease virus isolated from Suburbs of Karachi-Pakistan. Pakistan J. Zool 2012; 44(2): 443-448.
MAHMOUD , H.A MOHAMED ; SACHIN KUMAR ; ANANDANPALDURAI AND SIBA, K., SAMAL .2011. Sequence analysis of fusion protein gene of Newcastle disease virus isolated from outbreaks in Egypt during 2006Virology Journal 2011, 8:237 doi:10.1186/1743-422X-8-237
MASEMASA J.I; KUNITOSHI IMAI; YASUYUKISANADA; NAOKO SANADA; NOBORU YUASA; TADAOIMADA; KENJI TSUKAMOTO AND SHIGEO YAMAGUCHI. 2002. phylogenetic analysis of Newcastle disease virus genotypes isolated in Japan , J.clini.microbiol., Vol.40, no.10, p. 3826-3830.
MILLER, P.J.; DECANINI, E.L.; AND AFONSO, C.L. 2010. Newcastle disease: evolution of genotypes and the related diagnostic challenges. Infect. Genet.Evol. 10: 26–35
NABILA O; SULTAN S; AHMED AI; IBRAHIM RS;AND SABRA M. 2014.Isolation and Pathotyping of Newcastle Disease Viruses from Field Outbreaks among Chickens in the Southern Part of Egypt 2011- 2012. Global Veterinaria 2014; 12(2): 237- 243.
OIE .2018. OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals Ch. 2.3.4.Newcastle Disease.
OSMAN, N. SERAGELDEEN; S. AHMED I. AHMED; RAGAB S. IBRAHIM AND MAHMOUD SABRA .2014. Isolation and Pathotyping of Newcastle Disease Viruses from Field Outbreaks among Chickens in the Southern Part of Egypt. Department of Poultry Diseases, Faculty of Veterinary Medicine, South Valley University, Qena 83523, Egypt.
RAZMARAII,N.;TOROGHI,R.;BABAEI.H.;KHALILI,I.;SADIGHETEGHAD,S;AND FROGHY,L. 2012.Immunity of commercial, formaldehyde and binary ethylenimine inactivated Newcastle disease virus vaccines in specific pathogen free chicken archives of razi institute, vol.67,no.1:19-25.: Archives of Razi Institute, Vol. 67, No. 1, June (2012): 19- 25.
ROLA R.ALI.; MOKHTAR M ALI AND AMANI A. SALEH .2016. Characterization of Recent Isolates of Newcastle Diseasevirus Thesis ,Faculty of Veterinary Medicine Suez Canal University.Department of Virology.