Propagation, Purification And Molecular Characterization Of RVF virus (ZH 501) Strain For Vaccine Production In Egypt

E. M, Abo Hatab; Ali, M H; M. H., Atwa; D.M., Abul Magd; Soudy, Ahmed F.; Moussa, Saad A.

doi:10.21608/javs.2019.62672

Propagation, Purification And Molecular Characterization Of RVF virus (ZH 501) Strain For Vaccine Production In Egypt

Document Type : Original Article

Authors

¹ Animal Health Research Institute, Dokki, Giza, Egypt

² Veterinary Serum and Vaccine Research Institute, Abassia, Cairo, Egypt

10.21608/javs.2019.62672

Abstract

Rift Valley Fever (RVF) is an endemic disease in Egypt causing disease in animals and humans since the 1977. Full molecular description of the national vaccine strain is a strategic concern to ensure the antigenic makeup of local vaccine is adequate to provide the required protective immunity for the vaccinated animal species and as a tool of final product quality control. Molecular characterization and phylogenetic analysis of Gc gene of M segment of RVF-ZH501 strain which used in local vaccine production in veterinary serum and vaccine research institute (VSVRI) were carried out in this study. RVF-ZH501 strain was propagated by passages on BHK cell line and then purified my plaque purification technique. RT-PCR for amplification of the Gc gene of M segment. Sequence analysis of the obtained PCR product carried out by MEGA7 program. The result of the study revealed that RVF strain ZH501 Gc genomic content has 99.5 % molecular identity to the strains firstly isolated in Egypt in 1977, so still suitable for vaccine production.

Keywords

Main Subjects

Virology

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