Endosulfan Exposure Reduced Fertilization, Hatching, and Survival Rate of the Lemon Fin Barb Hybrid Eggs and Larvae

Document Type : Original Article

Authors

1 Department of Veterinary Preclinical Sciences, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia

2 Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia

3 Department of Aquaculture, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Selangor Darul Ehsan, Malaysia.

4 Department of Pharmaceutical Life Sciences, Faculty of Pharmacy, Universiti Teknologi MARA, Selangor Branch, Puncak Alam Campus, Puncak Alam, Selangor Darul Ehsan, Malaysia; Atta-ur-Rahman Institute for Natural Product Discovery, Universiti Teknologi MARA, Selangor Branch, Puncak Alam Campus, 42300 Puncak Alam, Selangor Darul Ehsan, Malaysia

Abstract

Endosulfan (ES) is an organochlorine insecticide that has been extensively used in agricultural production. Despite being banned globally, the production and illegal use of ES continue in certain countries, raising concerns about their impact on the environment and human health. The aim of this study was to examine the effect of ES on the fertilization, hatching, and survival rate of the lemon fin barb hybrid (LFBH; Hypsibarbus wetmorei × Barbonymus schwanenfeldii) eggs and larvae. A pair of LFBH was used as the broodstock. The sperm and eggs harvested via the stripping method were mixed and exposed to different concentrations of ES (0 ppm, or control; 0.01 ppm, 0.1 ppm, or 1 ppm). The fertilization and hatching rates were evaluated in vitro at 3 and 18 hours post-exposure, respectively. The survival rate of the larvae was assessed at 24, 48, and 72 hours post-hatching. The fertilization and hatching rates of the LFBH eggs treated with 1 ppm ES (44.24±4.6% and 18.54±2.8%, respectively) were significantly lower (p < /em><0.05) than the control (63.35±5.8% and 46.76±1.3%, respectively). The main effect of treatment and time on the survival rate of the larvae was significant (p < 0.019) within three days post-hatchlings, where the survival rate of larvae exposed to 1 ppm was significantly lower (p < 0.05) than the control at every time interval. Overall, ES exposures displayed a detrimental effect on the early development and survival of the LFBH eggs and larvae.

Keywords

Main Subjects

References

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Journal of Applied Veterinary Sciences
Volume 10, Issue 1
January 2025
Pages 132-139

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  • Receive Date: 28 November 2024
  • Revise Date: 27 December 2024
  • Accept Date: 01 January 2025

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