Antimicrobial Resistance of E. coli Isolated from Broiler Flocks and Slaughterhouses in Batna District, Algeria

Document Type : Original Article

Authors

1 Laboratoryof Epidemio-surveillance, Health and Reproduction, Cell Therapy of Domestic and Wild Animals, University of Chahid Bendjedid 3600, El Tare, Algeria

2 Laboratory of Epidemiology-surveillance, Health, Production & Reproduction, Cell Therapy of Domestic and Wild Animals, Department of Veterinary Sciences, Faculty of Nature and Life Sciences, University of Chadli Bendjedid, 36000. El Tarf, Algeria

3 Laboratory of Health, Animal Production and Environment (ESPA), Institute of Veterinary and Agricultural Sciences, University of Batna1, Algeria.

4 Department of Veterinary Sciences, Institute of Veterinary and Agricultural Sciences, University of Batna1, Algeria Laboratory of Biotechnology of Bioactive Molecules and Cellular Physiopathology (LBMPC), University of Batna2, Algeria

Abstract

In the last few years, the poultry sector in Batna has faced the emergence of atypical diseases. Many professionals suspect unusual viral infections like Newcastle disease, infectious bronchitis, and avian influenza. These diseases are often associated with colibacillosis and salmonellosis, both of which have developed atypical, multi-drug resistance. combat Escherichia (E.) coli superinfections, a study targeted broiler chickens from 80 flocks and two slaughterhouses in the Batna region. Researchers collected organ samples, including neck skin, from both sick animals and those intended for slaughter, isolating 100 E. coli strains—50 from flocks and 50 from slaughterhouses. antibiotic resistance profiles of E. coli isolated from livestock were 62.5% (50/80), revealing moderate resistance rates to several antibiotics. Specifically, resistance was noted for tetracycline (62%), doxycycline (56%), enrofloxacin (44%), ampicillin (46%), amoxicillin (40%), and sulfamethoxazole-trimethoprim (34%). Resistance to colistin showed a lower rate of 20%; amoxicillin-clavulanate and ticarcillin-clavulanate had a resistance of 12% and 8%, respectively. While gentamicin and chloramphenicol had even lower rates at 8% and 6%. Notably, 94% of strains were sensitive to chloramphenicol, and 92% were sensitive to gentamicin. Additionally, 28% of strains were resistant to three antibiotics, and 18% were resistant to four. Escherichia coli isolated from the slaughterhouse was 83.3% (50 / 60) demonstrated higher antibiotic resistance than those from herds. These isolates were resistant to tetracycline, ampicillin, enrofloxacin, and doxycycline, at 82%, 80%, 78%, and 74%, respectively, and had moderate resistance to nalidixic acid, chloramphenicol, sulfamethoxazole-trimethoprim (SXT), and amoxicillin; resistance rates for colistin, gentamicin, and TTC were lower. In terms of multidrug resistance, 42% of the slaughterhouse strains were resistant to five antibiotics, 18% to four, and 14% to two. Nevertheless, these strains remained highly sensitive to colistin and gentamicin.

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References

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Journal of Applied Veterinary Sciences
Volume 10, Issue 1
January 2025
Pages 22-31

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History

  • Receive Date: 28 September 2024
  • Revise Date: 19 November 2024
  • Accept Date: 06 December 2024

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