Assessment of Developmental Competence of Holstein Bulls Spermatozoa upon Addition of Aloe Vera Raw Extract during In Vitro Capacitation

Document Type : Original Article

Authors

Theriogenology Department, faculty of veterinary medicine, Beni suef university, Beni suef, Egypt

Abstract

The study aimed to evaluate the effect of the addition of aloe vera raw extract on the capacitation of bovine spermatozoa during the in vitro fertilization process. Hyper activated motility (HAM) and acrosome reaction (AR) of sperm cells, as well as in vitro fertilization and cleavage rates are the main parameters used to estimate the aloe vera’s effect on bovine spermatozoa’s fertilizing ability. In the current study, two different concentrations of aloe vera (5 and 10 µg/ml) were used. Frozen thawed semen spermatozoa were subjected to a swim-up technique to separate the motile spermatozoa and capacitated in modified Sperm Tyrode’s albumin lactate pyruvate (S-TALP) medium supplemented with heparin only without aloe vera treatment (positive control), heparin+5µg/ml aloe vera, heparin+10µg/ml aloe vera, and finally the negative control tube, which was supplemented with 5µg/ml aloe vera without adding heparin. Sperm cells were incubated for 90 minutes at 39C0 in a 5% CO2 incubator and evaluated every 30 minutes at intervals. Cumulus oophorus complexes (COCs) were matured in a 5% CO2 incubator at 39C0 and inseminated in vitro with frozen-thawed bull sperm at the above concentrations. The inseminated oocytes were incubated at 39C0 in a 5% CO2 incubator for 24 hours and then examined for evidence of fertilization. The current study revealed that the aloe vera concentration of 10µg/ml at incubation time (60 minutes) had the best effect on sperm capacitation and acrosome reactions. The overall percentage of spermatozoa with progressive motility (PM) had declined across all groups as time progressed, with a significantly lower value found at the above concentration, as well as the effect of sperm treated with aloe vera on fertilization rate. The results showed that the proportion of fertilized oocytes was significantly increased at the aloe vera concentration of 10µg/ml compared to the other concentrations (5µg/ml), positive control, and negative control, respectively. In conclusion, treatment of bull spermatozoa with 10 µg of aloe vera extract/ml semen was considered the most efficient concentration for enhancement of sperm capacitation as a high IVF rate was obtained in Holstein Bull.

Keywords

Main Subjects

References

ABD-ALLAH, S.M. 2009. Successful cryopreservation of buffalo ovaries using in situ oocyte cryopreservation. Veterinaria Italiana, 45(4), 507-512.
ABD-ALLAH, S.M., RALAN, M., SULIMAN, H., ESSAM, T., and SALEM, H.F., 2017.  Preparation of progesterone nanoparticles and evaluation of its effect on the capacitation of Bovine spermatozoa used in the in Vitro Fertilization. International Journal of Drug Delivery, 9(4), 125-131. http://www.arjournals.org/index.php/ijdd/article/view/2224/pdf.
ABDEL-HALIM, B.R. 2018.  Protective effect of Chitosan nanoparticles against the inhibitory effect of linoleic acid supplementation on maturation and developmental competence of bovine oocytes. Theriogenology, 114,143-148. https://doi.org/10.1016/j.theriogenology.2018.03.032
AITKEN, R.J. 2017. Reactive oxygen species as mediators of sperm capacitation and pathological damage. Molecular Reproduction and Development, 84, 1039-1052. https://doi.org/10.1002/mrd.22871
ANDRIELLE THAINAR MENDES CUNHA, FARIA, O.A.C., and GUIMARÃES, A.L.S., 2017. Bovine Sperm Capacitation: Physiological Changes and Evaluations. JSM In vitro Fertilization, 2(1), 1011. https://www.jscimedcentral.com/jounal-article-info/JSM-Invitro-Fertilization/Bovine-Sperm-Capacitation%3A-Physiological-Changes-and-Evaluations--6007#
Behmanesh, M.A., Najafzadehvarzi, H., and Poormoosavi, S.M., 2018. Protective Effect of Aloe Vera Extract against Bisphenol A Induced Testicular Toxicity in Wistar Rats. Cell Journal (Yakhteh), 2, 278-283. https://doi.org/10.22074%2Fcellj.2018.5256
CHAUHAN, S., GUPTA, K.C., and AGRAWAL, M., 2014. Application of Biodegradable Aloe Vera gel to control post-harvest decay and longer the shelf life of Grapes. International Journal of Current Microbiology and Applied Sciences, 3,632-642. http://www.ijcmas.com/
CHOI, S., and CHUNG, M.H., 2003. A review on the relationship between Aloe Vera components and their biologic effects. In Semin integ Med, 1, 53-62.  https://doi.org/10.1016/S1543-1150(03)00005-X
COMBELLES, C., and ALBERTINI, D., 2003. Assessment of oocyte quality following repeated gonadotropin stimulation in the mouse. Biology of Reproduction, 68,812-821. https://doi.org/10.1095/biolreprod.102.008656
DE LAMIRANDE, E., LECLERC, P., and GAGNON, C., 1997. Capacitation as a regulatory event that primes spermatozoa for the acrosome reaction and fertilization. Molecular Human Reproduction, 3,175-94. https://doi.org/10.1093/molehr/3.3.175
DEBNATH, T., GHOSH, M., LEE, Y.M., NATH, N.C.D., LEE, K.G., and LIM, B.O., 2018.  Identification of phenolic constituents and antioxidant activity of Aloe barbadensis flower extracts. Food and Agricultural Immunology, 29, 27-38. https://doi.org/10.1080/09540105.2017.1358254
DEMOTT, R.P., and SUAREZ, S.S., 1992. Hyper activated sperm progress in the mouse oviduct. Biology of Reproduction, 46, 779-85. https://doi.org/10.1095/biolreprod46.5.779
FAKHRILDIN, M.B. and SODANI, F.I.J. 2014. Effect of Aloe Vera on its in vitro human sperm parameters for asthenozoospermic patients. Journal of Thi-Qar Science, 5(1), 8-13. https://www.researchgate.net/publication/318795994_Effect_of_Aloe_vera_extracts_on_in_vitro_human_sperm_parameters_for_asthenozoospermic_patients
FUJINOKI, M., SUZUKI, T., TAKAYAMA, T., SHIBAHARA, H., and OHTAKE, H., 2006. Profiling of proteins phosphorylated or dephosphorylated during hyper activation via activation on hamster spermatozoa. Reproductive Medicine and Biology, 5(2), 123-135. https://doi.org/10.1007/BF03016148
GABRIEL, N.N., QIANG, J., MA, X.Y., HE, J., XU, P., and LIU, K., 2015. Dietary Aloe Vera improves plasma lipid profile, antioxidant, and hepatoprotective enzyme activities in GIFT-tilapia (Oreochromis niloticus) after Streptococcus iniae challenge. Fish Physio Bio, 41, 1321-1332. https://doi.org/10.1007/s10695-015-0088-z
GARAGOZLOO, P., and AITKEN, R.J., 2011. The role of sperm oxidative stress in male infertility and the significance of oral antioxidant therapy. Human Reproduction, 26, 1628-40. https://doi.org/10.1093/humrep/der132
GHOTBABADI, F.S., ALIZADEH, A., ZADEHBAGHERI, M., KAMELMANESH, M.M., and SHAABANI, M., 2012. Phytochemical composition of the essential oil, total phenolic content, antioxidant and antimicrobial activity in Iranian Satureja sahendica Bornm. At different ontogenesis conditions. Journal of Medicinal Plants Research, 6, 3525-3534. http://dx.doi.org/10.5897/JMPR11.374
GUVEN, M., GOLGE, U.H., ASLAN, E., SEHITOGLU, M.H., ARAS, A.B., AKMAN, T., and COSAR, M., 2016. The effect of aloe Vera on ischemia—Reperfusion injury of sciatic nerve in rats. Biomed Pharma, 79, 201-207. https://doi.org/10.1016/j.biopha.2016.02.023
HASHEMI, S.A., MADANI, S.A., and ABEDIANKENARI, S., 2015. The review on properties of Aloe Vera in healing of cutaneous wounds.  BioMed Research International, 2015, 714216. https://doi.org/10.1155/2015/714216
HENSLEIGH, H.C., and HUNTER, A.G., 1985. IVM of bovine cumulus enclosed primary oocytes and their subsequent IVF and cleavage. Journal of Dairy Science, 68, 1456-1462. https://doi.org/10.3168/jds.S0022-0302(85)80983-8
HO, H.C., and SUAREZ, S.S., 2001. Hyper activation of mammalian spermatozoa: function and regulation. Reproduction, 122,519-26.  https://doi.org/10.1530/rep.0.1220519
HU, J.H., LI, Q.W., ZHANG, T., and JIANG, Z.L., 2009. Effect of Gynostemma Pentaphyllum Polysaccharide on boar spermatozoa quality following freezing-thawing. Cryobiology, 59, 244–249. https://doi.org/10.1016/j.cryobiol.2009.04.008
JASEM, E., and NASIM, J., 2011. Spermatogenic activity Aloe Vera in adult male rat. Pharmacology online, 2, 886-889. https://www.researchgate.net/publication/265152041_Spermatogenic_activity_of_Aloe_vera_in_adult_male_rats
JOSÉ LUIS ROS-SANTAELLA and ELIANA PINTUS., 2021. Plant Extracts as Alternative Additives for Sperm Preservation. Antioxidants, 10, 772.https://doi.org/10.3390/antiox10050772
KEDARNATH KAMBLE KAVERI, M., CHIMKOD VISHWANATH, B., and PATIL, C.S., 2013. Antimicrobial Activity of Aloe Vera Leaf Extract. International Journal of Applied Biology and Pharmaceutical Technology, 4, 286.
KING, S.S., SPEISER, S.A., JONES, K.L., APGAR, G.A., and WESSELS, S.E., 2006. Equine spermatozoal motility and fertility associated with the incorporation of d-(+)-mannose into semen extender. Theriogenology, 65, 1171–1179. https://doi.org/10.1016/j.theriogenology.2005.08.002
KITIYANANT, Y., CHAISALEE, B., and PAVASUTHIPAISIT, K., 2002. Evaluation of the acrosome reaction and viability in buffalo spermatozoa using two staining methods: the effects of heparin and calcium ionophore A23187. International Journal of Andrology, 25(4), 215-222. https://doi.org/10.1046/j.1365-2605.2002.00350.x
LARSON, J.L., and MILLER, D.J., 1999. Simple histochemical stain for acrosomes on sperm from several species. Molecular Reproduction and Development, 52(4), 445-449. https://doi.org/10.1002/(SICI)1098-2795(199904)52:4%3C445::AID-MRD14%3E3.0.CO;2-6
LEUNG, M.Y.K., LIU, C., ZHU, L.F., HUI, B., and FUNG, K.P., 2004. Chemical and biological characterization of a polysaccharide biological response modifier from Aloe Vera L. var  chinensis (Haw) Berg. Glycobiology, 14, 501-510. https://doi.org/10.1093/glycob/cwh050
LOPEZ-CERVANTES, J., SÁNCHEZ-MACHADO, D.I., CRUZ-FLORES, P., MARISCALDOMÍNGUEZ, M.F., DE LA MORA-LÓPEZ, G.S., and CAMPAS-BAYPOLI, O.N., 2018. Antioxidant capacity, proximate composition, and lipid constituents of Aloe Vera flowers. Journal of Applied Research on Medicinal and Aromatic plant, 10, 93-98. https://doi.org/10.1016/j.jarmap.2018.02.004
MINA MAHMOODI POUR, 2019. In vitro Spermicidal Effects of Hydroalcoholic Crude Aloe Vera Leaf Extract on Animal Sperm Motility. International Conference on Biomedical Sciences (ICBMS19), ISBN 978-600-98459-5-8. https://www.greennetwork-events.com/all_images/file_193.pdf
MOGAS, T., MARTINO, A., PALOMO, M.J., and PARAMIO, M.T., 1992. Effect of method of recovery on the number and type of oocytes obtained for IVM. Journal of Reproduction and Fertility, 9, 53.
Mortimer, S.T., and Mortimer, D., 1990. Kinematics of human spermatozoa incubated under capacitating conditions. Journal of Andrology, 11, 195-203. https://doi.org/10.1002/j.1939-4640.1990.tb03228.x
NAEEM ERFANI MAJD, MOHAMMAD REZA TABANDEH, SHIMA HOSSEINIFAR, and MAHIN SADEGHI, 2021. Protective Effect of Aloe Vera Gel against Cisplatin-Induced Testicular Damage, Sperm Alteration and Oxidative Stress in Rats. International Journal of Fertility and Sterility, 15 (3), 210-218. https://doi.org/10.22074/ijfs.2020.134691
PADAMVEER SINGH, SANJAY AGARWAL, HARJYOTE SINGH, PAWAN KUMAR VERMA, A. K. and PANDEY SHARAD KUMAR., 2020. Antioxidant Effects of Aloe Vera as Semen Additive in Cryopreservation of Cattle Bull Semen. International Journal of Current Microbiology and Applied Sciences, 9(9), 1625-1635. https://doi.org/10.20546/ijcmas.2020.909.202
PARRISH, J.J., SUSKO-PARRISH, J.L., LIEBFRIED-RUTLEDGE, L., CRISTER, E.S., EYESTONE, W.H., and FRIST, N.L., 1986. Bovine in vitro fertilization with frozen-thawed semen. Theriogenology, 25, 591- 600. https://doi.org/10.1016/0093-691X(86)90143-3
PFEIFER, H., CONRAD, M., ROETHLEIN, D., KYRIAKOPOULOS, A., BRIELMEIER, M., BORNKAMM, G.W., and BEHNE, D., 2001. Identification of a specific sperm nuclei selenoenzyme necessary for protamine thiol cross-linking during sperm maturation. The FASEB Journal, 15, 1236-1238. https://doi.org/10.1096/fj.00-0655fje
PINTO, S.C.C., ALMEIDA, D.S., ALVES, M.B.R., FLOREZ-RODRIGUEZ, S.A., ABREU JÚNIOR, G.S., ALVES, N., CELEGHINI, E.C.C., LASKOKI, L.M., and SOUZA, F.A. 2020. Does supplementation of vitamin C, reduced glutathione or their association in semen extender reduce oxidative stress in bovine frozen semen? Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 72, 9–17. https://doi.org/10.1590/1678-4162-11293
QUISPE, C., VILLALOBOS, M., BORQUEZ, J., and SIMIRGIOTIS, M., 2018. Chemical Composition and Antioxidant Activity of Aloe Vera from the Pica Oasis (Tarapac´a, Chile) by UHPLC-Q/Orbitrap/MS/MS. Journal of Chemistry, 1-12. https://doi.org/10.1155/2018/6123850
RAJASEKARAN, S., SIVAGNANAM, K., and SUBRAMANIAN, S., 2005. Antioxidant effect of Aloe Vera gel extract in streptozotocin-induced diabetes in rat. Pharm Rep, 57, 90-96.
ROS-SANTAELLA, J.L., DOMÍNGUEZ-REBOLLEDO, A.E., and GARDE, J.J., 2014. Sperm flagellum volume determines freezability in red deer spermatozoa. PLoS ONE, 9, e112382. https://doi.org/10.1371/journal.pone.0112382
SACAN, O., AKEV, N., and YANARDAG, R., 2017. In vitro inhibitory effect of Aloe Vera (L.) Burm. f. leaf extracts on the activity of some enzymes and antioxidant activity. Indian Journal of Biochemistry and Biophysics, 54, 82-89. http://nopr.niscpr.res.in/handle/123456789/42894
SHAHIDI, F., WANASUNDARA, P.K., and JANITHA, P.D., 1992. Phenolic antioxidants. Critical Reviews in Food Science and Nutrition, 32, 67-103. https://doi.org/10.1080/10408399209527581
SHAHRAKI, A., SHAHKARI MOJAHED, A., and AFSHAR-GOLI, J., 2014. The effects of hydroalcoholic extract of Aloe Vera gel on spermatogenesis of adult male rats. International Journal of Biosciences, 5(7), 158-165. http://dx.doi.org/10.12692/ijb/5.7.158-8
SPSS. (2011). Statistical Package for Social Science. Computer Program. Version 22.
SUBBIAH, R., KARURAN, S., and SORIMUTHU, S., 2005. Antioxidant effect of Aloe Vera gel extract in streptozotocin induced diabetes in rats. Institute of Pharmacology Polish Academy of Sciences, 57, 90-96. https://pubmed.ncbi.nlm.nih.gov/15849382
TAUKOORAH, U., and Fawzi MAHOMOODALLY, M., 2016. Crude Aloe Vera Gel Shows Antioxidant Propensities and Inhibits Pancreatic Lipase and Glucose Movement in vitro. Journal of Advanced Biomedical and Pharmaceutical Sciences, 2016, 3720850. https://doi.org/10.1155/2016/3720850
WALEED, F.A. MAREI, WAEL A. KHALIL, ANIL, P.G. PUSHPAKUMARA, MOSTAFA, A. EL-HARAIRY, AHMED, M.A. ABO EL-ATTA, CLAIRE WATHESD, D. and ALI FOULADI-NASHTAE, 2018. Polyunsaturated fatty acids influence offspring sex ratio in cows. International Journal of Veterinary Science and Medicine, 6, S36-S40. https://doi.org/10.1016/j.ijvsm.2018.01.006
WARD, F., RIZOS, D., BOLAND, M.P., and LONERGAN, P., 2003. Effect of reducing sperm concentration during IVF on the ability to distinguish between bulls of high and low field fertility: work in progress. Theriogenology, 59, 1575-1584. https://doi.org/10.1016/S0093-691X(02)01202-5
WOJDYŁO, A., OSZMIAŃSKI, J., and CZEMERYS, R., 2007. Antioxidant activity and phenolic compounds in 32 selected herbs. Food Chemistry, 105, 940-949. https://doi.org/10.1016/j.foodchem.2007.04.038
YONG, S.Y., NGUANG, S.I., and KARI, A., 2017. Comparison between the effect of egg yolk based extender and Aloe Vera (Aloe Barbadensis)-based extender on red tilapia (Oreochromis Niloticus) sperm quality. Journal of fundamental and applied sciences, 9, 813-820. http://dx.doi.org/10.4314/jfas.v9i2s
YUANYUAN HE, YILE WANG, HENGDE ZHANG, YONG ZHANG and FUSHENG QUAN, 2020. Alpha-    lipoic acid improves the maturation and the developmental potential of goat oocytes in vitro. Reproduction in Domestic Animals, 56, 545-554. https://doi.org/10.1111/rda.13892
YUKSEL, Y., GUVEN, M., KAYMAZ, B., SEHITOGLU, M.H., ARAS, A.B., AKMAN, T., TOSU, M., and COSAR, M., 2016. Effects of aloe Vera on spinal cord Ischemia–Reperfusion injury of rats. Journal of Investigate Surgery, 29, 389-398. https://doi.org/10.1080/08941939.2016.1178358
ZAREIE, K., FARSHAD, A., ROSTAMZADEH, J., AZIMI, G., and ARIYAN. F., 2021. Freezability of Goat Epididymal Sperm using Aloe Vera Extract and Trehalose in Diluents. AustinJournal of Veterinary Science & Animal Husbandry, 8(2), 1078. http://www.austinpublishinggroup.com/
ZINI, A., SAN GABRIEL, M., and LIBMAN, M., 2010. Lycopene supplementation in vitro can protect human sperm deoxyribonucleic acid from oxidative damage. Fertility and Sterility, 94, 1033–6. https://doi.org/10.1016/j.fertnstert.2009.04.004
 

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  • Receive Date: 22 June 2023
  • Revise Date: 23 July 2023
  • Accept Date: 13 August 2023

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