Abodalal, S., Ismail, M., El Rawy, E. (2023). Preparation of a Combined Inactivated Vaccine against Riemerella anatipestifer and Duck Viral Hepatitis. Journal of Applied Veterinary Sciences, 8(1), 26-31. doi: 10.21608/javs.2022.163930.1179
Samah El Sayed Ali Abodalal; Mamoud T.A. Ismail; Eman Mohamed El Rawy. "Preparation of a Combined Inactivated Vaccine against Riemerella anatipestifer and Duck Viral Hepatitis". Journal of Applied Veterinary Sciences, 8, 1, 2023, 26-31. doi: 10.21608/javs.2022.163930.1179
Abodalal, S., Ismail, M., El Rawy, E. (2023). 'Preparation of a Combined Inactivated Vaccine against Riemerella anatipestifer and Duck Viral Hepatitis', Journal of Applied Veterinary Sciences, 8(1), pp. 26-31. doi: 10.21608/javs.2022.163930.1179
Abodalal, S., Ismail, M., El Rawy, E. Preparation of a Combined Inactivated Vaccine against Riemerella anatipestifer and Duck Viral Hepatitis. Journal of Applied Veterinary Sciences, 2023; 8(1): 26-31. doi: 10.21608/javs.2022.163930.1179
Preparation of a Combined Inactivated Vaccine against Riemerella anatipestifer and Duck Viral Hepatitis
1Newcastle Disease Research Department, Veterinary Serum and Vaccine Research Institute (VSVRI), Abbasia, Agricultural Research Center (ARC), Cairo, Egypt
2Aerobic Bacteria Research Department, Veterinary Serum and Vaccine Research Institute (VSVRI), Abbasia, Agricultural Research Center (ARC), Cairo, Egypt.
Receive Date: 19 September 2022,
Revise Date: 21 October 2022,
Accept Date: 15 November 2022
Abstract
Riemerella anatipestifer (R. anatipestifer) infection and duck virus hepatitis (DVH) are enormous dangers for the duck industry and its investment. So, in the current study, a combined inactivated vaccine against both of them was prepared to combat their adverse effect. One hundred and thirty-three ducks of one-day-old of age were used and grouped randomly into four groups. Group (1) was vaccinated with R. anatipestifer vaccine, group (2) was vaccinated with DHV vaccine, group (3) was vaccinated with the prepared combined vaccine of both and finally, group (4) was kept as a negative control. Vaccination was at one day old of age. The vaccinated groups with Riemerella vaccine had 72.7% protection against challenges with the virulent strain with the highest antibody titers in 6th week as measured by the indirect Hemagglutination test. The control group had 90.9% mortality when challenged against R. anatipestifer, with no detectable antibody titers. DVH-vaccinated groups exhibited their highest serum-neutralizing antibody titers by the 5th and 6th weeks post-vaccination. The Control group had no detectable antibody titers against DVH. Statistically, it was clear that there were no significant differences between the results of different groups vaccinated with combined or single vaccines of the same agent. Briefly, combined vaccines of R. Anatipestifer and duck viral hepatitis have harmonized effects with the priority to decrease the stress on birds and workers. Besides its efficiency, the economic side as providing one-shot vaccines instead of each one separately.
ABOUL SAOUD, S. A., MANAL S. MAHMOUD, EMAN S. AHMED and AMAL M. EL-SAWAH, 2010. 10th Scientific Veterinary Medical Zagaziz Conference., 300 – 309.
BEBARS, A.S. 2000. Characterization of Pasteurella of duck origin by using protein profile analysis and DNA fingerprinting. MD. Thesis, Microbiology, Fac. Vet. Med., Cairo Univ.
CARTER, G.R., and COLE, J.R., 1990. Diagnostic procedures in veterinary bacteriology and mycology. 5th Ed. Academic Press, Inc. Harcourt Brace Jovanovich, Publishers, London, New York.
CHEN, Y.P., LEE, S.H., CHOU, C.H., and TSAI, H.J., 2012. Detection of florfenicol resistance genes in Riemerella anatipestifer isolates from ducks and geese. Vet. Microbiol., 154: 325-331. DOI: 10.1016/j.vetmic.2011.07.012
DING, C., and ZHANG, D., 2007. Molecular analysis of duck hepatitis virus type 1. Virology, 361 (1): 9-17. DOI:10.1016/j.virol.2007.01.007
DOHA ABD ALRAHMAN AHMED, MOSTAFA SAIF ELDIN, RAGAB SAYED IBRAHIM and, OMAR AMEN, 2021. Epidemiological and Molecular Studies on Riemerella Anatipestifer Infection in Ducks. Assiut Veterinary Medical Journal, 67(168): 61-74. DOI: 10.21608/avmj.2021.177849
EL-KOFFY M. A. 1997. Studies on the preparation and evaluation of a vaccine prepared from a local strain of duck virus hepatitis Ph.D. thesis, poultry and rabbit diseases, faculty of veterinary medicine, Cairo university.
EL-RAWY, E.M., KHADER, A.A., MAHMOUD, M.S., and ZAKI, E.S., 2020. Preparation and evaluation of combined oil adjuvant vaccine against duck pasteurellosis and Riemerella anatipestifer infection in ducks. International Journal of Veterinary Sciences and Animal Husbandry, 5 (1): 18-21.
GAMAL, F.Z., SOLIMAN, E.M., EL-NAGGAR, H.M., ABD EL-MONEIM, W.S., and HASSANNIN, A.I., 2021. Trial for preparation and evaluation of combined inactivated vaccine for the protection against Riemerella anatipestifer and Avian Influenza (h5n1) in ducks. Adv. Anim. Vet. Sci, 9(4): 490-499. DOI | http://dx.doi.org/10.17582/journal.aavs/202
IBM CORP. RELEASED, 2019. IBM SPSS Statistics for Windows, Version 26.0. Armonk, NY: IBM Corp
KALETA, E.F. 1988. Duck viral hepatitis type 1 vaccination: monitoring of the immune response with a microneutralization test in Pekin duck embryo kidney cell cultures. Avian Pathology,17(2):325-332. https://doi.org/10.1080/03079458808436451
KOZDRU, W., CZEKAJ, H., and LOREK, M., 2014. Outbreak of duck viral hepatitis in duckling flocks in Poland-case report. Journal of Veterinary Research, 58 (4): 513-515. DOI: https://doi.org/10.2478/bvip-2014-0078
LIU, H., WANG, X., DING, C., HAN, X., CHENG, A., WANG, S., and YU, S., 2013. Development and evaluation of a trivalent Riemerella anatipestifer-inactivated vaccine. Clinical and Vaccine Immunology, 20 (5): 691-697. DOI: 10.1128/CVI.00768-12
MERVAT, A. EL-KOFFY, ABOU EL-KHAIR, M.A., ABD EL-KHALECK, M.A., and ABD EL-WANIS, N.A., 2000. Trials for production of alhydragel inactivated duck hepatitis virus vaccine. SCVMJ. 3 (1): 215-221.
MERVAT A. EL-KOFFY; ABOU EL-KHAIR, MA; ABD EL-KHALECK, MA, and ABD EL-WANIS, N.A., 2005. Preparation and evaluation of combined inactivated aluminum hydroxide gel vaccine against duck virus hepatitis and duck plague disease private. Vet. Med. J. Giza. 53 (4): 901-909.
OIE, 2018. Manual of Diagnostic Tests and Vaccines for Terrestrial Animals chapter 3.3.8 Duck Virus Hepatitis.
SOMAN, M., NAIR, S.R., MINI, M., MANI, B.K., and JOSEPH, S., (2014). Isolation and polymerase chain reaction-based identification of Riemerella anatipestifer from ducks in Kerala, India. Veterinary World, 7 (10): 765. DOI: 10.14202/vetworld.2014.765-769
Stoute, S.T., Sandhu, T.S., and Pitesky, M.E., 2016. Evaluation of protection induced by Riemerella anatipestifer-E. coli O78 bacterin in white pekin ducks. Journal of Applied Poultry Research, 25 (2): 232-238.
SUBRAMANIAM, S., HUANG, B., LOH, H., TAN, H-M., CHUA K-L., FREY, J., and KWANG, J., 2000. Characterization of a predominant immunogenic outer membrane protein of Riemerella anatipestifer. Clin. Diagn. Lab. Immunol., 7: 168–174. DOI: 10.1128/cdli.7.2.168-174.2000
WANG, Y., GUO, Y., WANG, H., WU, Z., HONG, W., SUN, H., and ZHU, S., 2022. Protection of Ducklings from Duck Hepatitis A Virus Infection with ELPylated Duck Interferon-α. Viruses, 14 (3): 633. DOI: 10.3390/v14030633
ZANATY, A., HAGAG, N., SAMY, M., ABDEL-HALIM, A., A SOLIMAN, M., ARAFA, A.S., and NASIF, S., 2017. Molecular and pathological studies of duck hepatitis virus in Egypt. Journal of Veterinary edical Research, 24 (2): 194-204. DOI: 10.21608/jvmr.2017.43283
ZHANG, Y., CHEN, H., ZENG, X., WANG, P., LI, J., and WU, W., 2014. Levamisole enhances immunity in ducklings vaccinated against Riemerella anatipestifer. Microbiology and Immunology, 58 (8): 456-462.DOI: 10.1111/1348-0421.12169
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