Isolation and Antimicrobial Resistance Phenotype of Salmonella species from Ready-to-Eat Roasted Meat (Suya) in Ilorin, Kwara State, Nigeria

Oludairo, Oladapo Oyedeji; A.K., Balogun; O.B., Daodu; J.O., Aiyedun

doi:10.21608/javs.2022.154058.1166

Isolation and Antimicrobial Resistance Phenotype of Salmonella species from Ready-to-Eat Roasted Meat (Suya) in Ilorin, Kwara State, Nigeria

Document Type : Original Article

Authors

¹ Department of Veterinary Public Health and Preventive Medicine, University of Ilorin, Nigeria

² Department of Veterinary Microbiology, University of Ilorin, Nigeria

10.21608/javs.2022.154058.1166

Abstract

Salmonella are pathogenic bacteria that can contaminate food products during or after processing. They are among the most important food borne pathogens worldwide contaminating a wide range of animal products including meat products. Human illnesses due to this pathogen are attributed to poor biosecurity in production and improper processing/handling of meat and meat products. This is more likely where surveillance and regulatory control are weak. Ready-to-eat (RTE) foods, like roasted meat (Suya) in Ilorin, do not undergo any treatment to ensure its safety before consumption, therefore the risk of food borne disease occurrence must be considered if these pathogens are present in the food. This study aimed to evaluate the prevalence of Salmonella in Suya in Ilorin, Nigeria. A total of 102 Suya samples were collected and analysed for the presence of Salmonella. Samples were obtained from 5 major markets, consisting of a total of 15 Suya retail outlets. Organism isolation and identification were done using conventional methods. Confirmation of suspected isolates was done using Microbact GNB 24E. Five samples (4.9%) were positive for Salmonella. Confirmed Salmonella isolates were evaluated in vitro for susceptibilities to 12 commonly used antimicrobial agents. All (100%) of the confirmed Salmonella isolates exhibited multiple resistance to at least 3 antibiotics and exhibited five antimicrobial resistance phenotypic patterns. The classes of antibiotics they were resistant to are: aminoglycosides, cephalosporin, penicillin and tetracycline. The isolation of Multiple Drug Resistant (MDR) Salmonella species in this study makes Suya potential vehicle for the transmission of Salmonella. Effort should be made to sensitize producers of Suya on the potential risk of the product being vehicles for the transmission of food borne pathogens and the need to maintain high level hygiene during production. The public should be educated to only patronize reliable Suya joints. Constant research work should be carried out to evaluate the level of contamination of ready to eat foods like Suya in order to be able to take informed decisions.

Keywords

Main Subjects

public health and preventive medicine

References

ABDULLAHI, I. O., UMOH, V. J., AMEH, J. B., and GALADIMA, M., 2014. Hazards associated with Kilishi preparation in Zaria, Nigeria. Nigerian Journal of Microbiology, 18: 338 - 345.

AIYEDUN, J.O., OLUDAIRO, O. O., OLORUNSHOLA, I. D., FURO, N. A., OLOWOLENI, F. R., ADAM, M., and SHOYINKA, S.V.O., 2017. Seroepidermiological Suvey of Bovine Brucellosis in Selected Fulani Herds in Kwara State, Nigeria. Journal of Advanced Veterinary and Animal Research, 4: 222 - 226. https://www.banglajol.info/index.php/JAVAR/article/view/33130

AKINYEMI, K. O., OSHUNDARE, Y. O., OYEYINKA, O. G., and COKER, A. O., 2012. A retrospective study of community-acquired Salmonella infections in patients attending public hospitals in Lagos, Nigeria. Journal of Infection in Developing Countries,6: 87-95. doi: 10.3855/jidc.2120.

AMEME, D. K., ABDULAI, M., ADJEI, E. Y., AFARI, E. A., NYARKO, K. M., ASANTE, D., KYE-DUODU, G., ABBAS, M., SACKEY, S., and WURAPA, F., 2016. Foodborne disease outbreak in a resource-limited setting: a tale of missed opportunties and implications for response. The Pan African Medical Journal, 23: 69.

CLINICAL LABORATORY STANDARDS INSTITUTE (CLSI), 2016. Performance Standards for Antimicrobial Susceptibility Testing. 26th informational supplement. CLSI document M100-S25. Clinical Laboratory Standards Institute, 950 West Valley Road, Suite 2500, Wayne, Pennsylvania, USA.

CLINICAL LABORATORY STANDARDS INSTITUTE (CLSI), 2020. M100 Performance Standards for Antimicrobial Susceptibility Testing 30th Edition.Clinical Laboratory Standards Institute, 950 West Valley Road, Suite 2500, Wayne, Pennsylvania, USA. Available at https://www.nih.org.pk/wp-content/uploads/2021/02/CLSI-2020.pdf. Accessed 29th August 2022.

CRUICKSHANK, R., DUGUID, J. P., MARMION, B. P., and SWAIN, R. H. A., 1975. Medical Microbiology. 12th Edition, 2: 199. Churchhill Livingstone, Edinburgh, London and New York.

DEMOGRAPHIA, 2018. Demographia World Urban Areas. Built-up Urban Areas or Urban Agglomerations.

EGBEBI, A. O., and MUHAMMAD, A. A., 2016. Microbiological Analysis of Ready-To-Eat Suya Meat Sold in Owo, Ondo State. International Journal of Innovative Biochemistry & Microbiology Research, 4: 11-15.

https://pdfslide.net/documents/microbiological-analysis-of-ready-to-eat-suya-meat-sold-in-owo-.html

EL JAKEE, J., ATA, N. S., ABD EL-MOEZ, S. I., KANDIEL, M. M., and RADWAN, N. M., 2014. Assessment of the Prevalence of Salmonellae in Food. International Journal of Current Microbiology and Applied Science, 3: 30 - 42.https://www.ijcmas.com/vol-3-3/J.EI%20Jakee,%20et%20al.pdf

ELVISS, N. C., and JORGENSEN, F., 2012. Investigations Prompted by the Discovery of High Levels of Salmonella in Fresh Herbs in the UK. In Hoorfar J., Case Studies in Food Safety and Authenticity (pp. 116 - 122). Woodhead Publishing Series in Food Science, Techhnology and Nutrition.

FAHIM, A. S., AHMED, A. A., IBRAHIM, A. E., and AHMED, Y. A., 2016. Prevalence of some foodborne microorganisms in meat and meat products. Benha Veterinary Medical Journal, 31: 213 – 219. https://bvmj.journals.ekb.eg/article_31300_363d7f49d41e3d9ba4c939f461471741.pdf.

FALEGAN, C. R., AKOJA, S. O., and OYAREKUA, M. A., 2017. Microbiological assessment of suya (sliced roasted beef) in Ado - Ekiti Metropolis, Ekiti State, Nigeria. MedCrave Online Journal of Biology and Medicine (MOJBM), 2: 266 - 269. DOI: 10.15406/mojbm.2017.02.00053.

FALLAH, S. H., ASGHARPOUR, F., NADERIAN, Z., and MOULANA, Z., 2013. Isolation and Determination of Antibiotic Resistance Patterns in Non-typhoid Salmonella spp. isolated from chicken. Enteric Pathogens, 10: 5812 - 9416. DOI: 10.17795/ijep9416.

FEASEY, N. A., DOUGAN, G., KINGSLEY, R. A., HEYDERMAN, R. S., and GORDON, M. A., 2012. Invasive non-typhoidal Salmonella disease: an emerging and neglected tropical disease in Africa. Lancet,379: 89 – 99.

https://doi.org/10.1016%2FS0140-6736(11)61752-2

FOOD AND AGRICULTURAL ORGANIZATION (FAO), 1992. Manual of Food Quality Control 4, Revuel. Microbiological analysis, F.A.O., Rome, Italy. Pp 338.

HASSANIN, F. S., REHAM, A. A., SHAWKY, N. A., and GOMAA, W. M., 2014. Incidence of Escherichia coli and Salmonella in Ready to eat Foods. Benha Veterinary Medical Journal,27: 84 - 91.

INTERNATIONAL ORGANIZATION OF STANDARDIZATION (ISO), 2007. International Standard, Microbiology of feed and animal feeding stuffs-horizontal method for the detection of Salmonella spp. Ammendment 1: Annex D: Detection of Salmonella spp in animal. Available at: www. aait.org.cn/web/images/upload/2013/07/11/20137111148308281.pdf. Accessed 24th June, 2022.

INYANG, C. U., IGYOR, M. A., and UMA, E. N., 2005. Bacterial quality of a smoked meat product (Suya). Nigerian Food Journal, 23: 239 - 242. DOI: 10.4314/nifoj.v23i1.33622.

JANTSCH, J., CHIKKABALLI, D., and HENSEL, M., 2011. Cellular aspects of immunity to intracellular Salmonella enterica. Immunological Reviews, 240: 85 – 95.

JAY, J. M. 2000. Modern Food Microbiology (6th ed., pp. 60 - 89). Gaithersburg. Maryland: Aspen Publishers Inc..

KONNE, F. E., MONSI T. P., and WOKEM G. N., 2018. Bacteriology of Suya Meat Sold in Bonny Local Government Area, Rivers State. Asian Journal of Medicine and Health, 10: 1 - 7.

MALICK, M. G., CARL, B., and ANDREW, J. P., 2018. Typhoid and paratyphoid fever: a call to action. Lippincott Williams & Wilkins Open Access,31: 440 – 448.

MOSHOOD, A. Y., TENGKU, H., and IBRAHIM, H., 2012. Isolation and Identification of Bacteria Associated with Balangu (Roasted Meat Product) Sold in Bauchi. Nigeria. IOSR Journal of Pharmacy, 2: 38 - 48.

NORMA, H., and SANTOS, G., 2018. Animals as Sources of Food-borne Pathogens: A Review. Animal Nutrition, 4: 250 - 255.

https://doi.org/10.1016/j.aninu.2018.04.006

OGBU, K. I., PRAM, V. A., CHUKWUDI, I. C., MOMOH, A. H., and AGWU, E. O., 2016. Survey of Bacterial and Fungal Contamination on Beef Suya sold in Jos and Environs. International Journal of Science and Applied Research, Volume 1. https://www.researchgate.net/publication/315720849_Survey_of_bacterial_and_fungal_contamination_on_beef_suya_sold_in_Jos_and_environs

OLADUNJOYE, Z. M., QUADRI, J. A., OJURE, M. A., and USIOBEIGBE, O. S., 2019. Isolation of Pathogenic Organisms from Suya Meat Obtained from Selected Local Governments in Ogun East Senatorial District of Ogun State, Nigeria. International Research in Medical and Health Sciences, 2: 1 - 7.

OLUDAIRO, O. O., KWAGA, J. K. P., DZIKWI A. A., and KABIR J., 2013a. The genus Salmonella, isolation and occurrence in wildlife. International Journal of Microbiology and Immunology Research, 1: 047-052.

OLUDAIRO, O. O., KWAGA, J. K. P., DZIKWI, A. A., and KABIR, J., 2019. Drug of choice in the treatment of multiple drug resistant (MDR) Salmonellae isolated from wildlife in Nigeria. Journal of Veterinary and Biomedical Science, 2: 170–180.

OLUDAIRO, O. O., KWAGA, J. K. P., DZIKWI, A. A., and KABIR, J., 2013b. Prevalence of Salmonella spp. in captive wildlife at the National Zoological Garden Jos, Nigeria. Current Research in Microbiology and Biotechnology, 6: 285-288.

OLUDAIRO, O. O., KWAGA, J. K. P., KABIR, J., ABDU, P. A., GITANJALI, A., PERRETS, A. CIBIN, V., LETTINI, A. A., and AIYEDUN, J. O., 2022. Review of Salmonella Characteristics, History, Taxonomy, Nomenclature, Non Typhoidal Salmonellosis (NTS) and Typhoidal Salmonellosis (TS). Zagazig Veterinary Journal, 50 (2): 160-171.

OLUDAIRO, O. O., KWAGA, J. K. P., KABIR, J., ABDU, P. A., GITANJALI, A., PERRETS, A., CIBIN V., LETTINI, A., and AIYEDUN, J. O., 2022b. "A review of the International Organization for Standardization (ISO) guidelines for the detection of Salmonella from faeces". Journal of Applied Veterinary Sciences, doi: 10.21608/javs.2022.146858.1158.

QUINN PJ, MARKEY BK, LEONARD FC, HARTIGAN P, FANNING S., and FITZPATRICK E., 1994. Clinical Veterinary Microbiology, Mosby, London.

RAJAGOPAL, R., and MINI, M., 2013. Outbreaks of salmonellosis in three different poultry farms of Kerala, India. Asian PacificJournal of Tropical Biomedicine , 3: 496 - 500.

TAFIDA, S. Y., KABIR, J., KWAGA, J. K. P., BELLO, M., UMOH, V. J., YAKUBU, S. E., NOK, A. J., and HENDRIKSEN, R., 2013. Occurrence of Salmonella in Retail Beef and Related Meat Products in Zaria, Nigeria. Food Control, 32: 119 – 124.

TESSARI, E. N. C., KANASHIRO, A. M. I., STOPPA, G. F. Z., LUCIANO, R. L., DE CASTRO, A. G. M., and CARDOSO ANA LUCIA, S. P., 2013. Important Aspects of Salmonella in the Poultry Industry and in Public Health. In Dr. Mahmoud, S. M., Salmonella - A Dangerous Food-borne Pathogens.

TSAKU, P. A., NGWAI, Y. B., PENNAP, G. R. I., ISHALEKU, D., IBRAHIM, T., NKENE, I. H., and ABIMIKU, R. H., 2019. Extended-Spectrum Beta-Lactamase-production in Escherichia coli isolated from door handles in Nasarawa State University, Keffi, Nigeria. Heliyon;5(8).

doi: 10.1016/j.heliyon.201e02177

WORLD HEALTH ORGANIZATION (WHO), 2015. WHO Estimates of The Global Burden of Foodborne Diseases. Retrieved July 24, 2022, from https://www.who.int/foodsafety/publications/foodborne_disease/fergreport/en/.