Factors Affecting on Production of Clostridium novyi type (B) Alpha Toxin

Ahmed, Marwa; Yehia, Marwa; Ahmed, Hala ElSawy

doi:10.21608/javs.2022.123292.1129

Factors Affecting on Production of Clostridium novyi type (B) Alpha Toxin

Document Type : Original Article

Authors

¹ Anaerobic Department, Veterinary Serum and Vaccine Research Institute (VSVRI), Agriculture Research Center (ARC), Egypt.

² Rift Valley Fever Department, Veterinary Serum and Vaccine Research Institute (VSVRI), Agriculture Research Center (ARC), Egypt.

³ Quality control lab (QCL) , Agriculture Research Center (ARC), Egypt.

10.21608/javs.2022.123292.1129

Abstract

Clostridium novyi type B is an anaerobic bacterium that causes black disease (infectious necrotic hepatitis). The alpha toxin is considered as a major virulence factor. The disease has been controlled using toxoid vaccines. Production of a high yield of bacterial toxin is very important in the preparation of effective toxoids. The present study aimed to follow the most satisfactory growth requirements to produce a high yield of alpha toxin. This study tested the effect of different concentrations of meat pieces (0. 0.5, 1.5, 2 and 2.5%), adding yeast extract in concentration of (0.5%), variable incubation times (24, 48, 72, 96 and 120 hours) and different pH values (7, 7.5 and 8).The results showed that the best media used for producing alpha toxin of Clostridium novyi type B was the one that contains yeast extract in the concentration of 0.5% with absence of meat pieces and adjusting pH between 7.5- 8 and incubation for 24- 48 hrs. that leads to high bacterial growth and toxin yield production optimum physical condition as incubated period 24- 48 hrs. and favorable pH for growth production and toxin yield of Clostridium novyi type B which lead consequently to produce a high potent Clostridium novyi vaccine.

Keywords

Main Subjects

Bacteriology

References

ARDEHALI, M., MOOSAWI, M., and PILEHCHIAN, R., 1992. Mass Production of Clostridium oedematiens Vaccine against Black Disease of Sheep Arch. Imt. Razi. 42/43, 85-89.

AQUINO, P. L. M., FONSECA, F. S., MOZZER, O. D., GIORDANO, R. C., and SOUSA, R., 2016. Optimization of the Production of Inactivated Clostridium novyi Type B Vaccine Using Computational Intelligence Techniques Jr Appl Biochem Biotechnol 179:895–909. Doi:10.1007/s12010-016-2038-3.

BALL, D.M., TASSELL, R.L.V., ROBERTS, M.D., HAHN,P. E., LYERLY, D.M., and WILKINS, T. D., 1993. Purification and characterization of alpha- toxin produced by Clostridium novyi type A. Infect. Immun. 61: 2912- 2918. Doi: 10.21608/eajbsc.2020.115949

BARD R. C., and MCCLUJNG L. S., 1948. Biochemical properties of the toxins of Clostridium novyi and Clostridium hemolyticum. Journal of bacteriology vol. 56 665- 670. Doi: 10.1128/jb.56.5.665-670.1984.

GADALLA, M.S., FARRAG, I., and SHARF, D., 1974. Effect of growth requirement on the improvement of clostridial vaccine. J. Egypt. Vet. Med. Ass. 34: 19-28.

GADALLA, M.S., IKBAL FARRAG, LOTFY, O., MAHMOUD, M.S., EL DANAF, N.A., DORREYA SHARAF and HUSSEIN, M.1971.The immunogenicity of alum precipitated multicomponent clostridial vaccine. J. Egypt. Vet. Med. Ass., 31 :135-150.

ISPOLATOVSKAYA, M.V. 1971. Type A Clostridium perfringens toxin. In Microbial Toxins, Vol. IIA. S. Kadis, T.C. Montie and S.J. Ajl, Eds. pp. 109-158. New York: Academic Press.

MARION LECLERC, LILA ELFOUL-BENSAID and ANNICK BERNALIER, 1998. Effect of Yeast Extract on Growth and Metabolism of H2-Utilizing Acetogenic Bacteria from the Human Colon. Current Microbiology Vol. 37 (1998), pp. 166–171. Doi: 10.1007/s002849900358.

MIYAKAWA-FERNANDEZ, M.E.; MARCELLINO, R., and UZAL, F.A., 2007. Clostridium perfringens type A toxin production in 3 commonly used culture media. J. Vet. Diagn. Invest. 19:184-186. Doi: 10.1177/104063870701900208.

MOHSEN F. NAJAFI, MOHSEN M. ARZ, BEHJAT MAJEDI and H. JANDAGHI, 2019. Induction of alpha toxin production and reduction of time of cultivation of Clostridium novyi for vaccine production. 20^th International Congress of Microbiology of Iran.

NAVARRO, M. AND UZAL, F.A. 2016. Infectious Necrotic Hepatitis. In Clostridial Diseases in Animals; JohnWiley & Sons, Inc.: Hoboken, NJ, USA, 2016;ISBN9781118728291.https://doi.org/10.1002/9781118728291.ch23.

NISHIDA, S AND NAKAGAWARA, G. 1964. Isolation of toxigenic strains of Clostridium novyi from soil. J. Bacteriol. 88:1636-1640.

Doi:https://doi.org/10.1128/jb.88.6.1636-1640.1964

NISHIDA, S., MURAKAMI, M AND YAMAGISHI, T. 1962. Chopped meat broth as a medium for the preparation of toxins by clostridia. I. Production of toxins by Cl. welchii. Japan. J. Microbiol. 6:33-40. Doi: 10.1111/J.1348-0421.1962.TB00236.X.

OKSCHE, A., NAKOV, R. AND HABERMAN, E. 1992. Pharamacological and biochemical studies of cytoskeletal changes in cultured cells after extracellular application of Clostridium novyi alpha- toxin. Infect. Immun. 60: 3002- 3006. Doi: 10.1128/iai.60.73002-3006.1992.

TOFALO, R. AND SUZZI, G. 2016. Yeasts. In The Encyclopedia of Food and Health, 1st ed.; Caballero, B., Finglas, P.M., Toldrá, F., Eds.; Academic Press: Oxford, UK, 2016; Volume 1, pp. 593–599. Doi: 10.1016/b978-0-12-384947-2.00762-5.

UNDERWOOD, W. J., BLAUWIEKEL, R., DELANO, M. L., GILLESBY, R., MISCHLER, S. A., and SCHOELL, 2015. Biology and Diseases of Ruminants (Sheep, Goats, and Cattle). Laboratory Animal Medicine, 623–694. doi:10.1016/b978-0-12-409527-4.00015-8

WHITFIELD, L.; CYPHER, E.; GORDON, S.; PAUWELS, F.; LING, J.; COLLETT, M. AND UZAL, F. 2015. Necrotic hepatitis associated with Clostridium novyi infection (black disease) in a horse in New Zealand. N. Z. Vet. J. 2015, 63, 177–179. Doi: 10.1080/00480169.2014.973461.