Assessment of Three Anesthetics Used for Pulmonary Pharmacological Studies in Rats

Adam, Gareeballah Osman

doi:10.21608/javs.2022.119861.1123

Assessment of Three Anesthetics Used for Pulmonary Pharmacological Studies in Rats

Document Type : Original Article

Author

Gareeballah Osman Adam

Assistant Professor at Department of Pharmacology and Toxicology, College of Veterinary Medicine, Sudan University of Science and Technology, Sudan.

10.21608/javs.2022.119861.1123

Abstract

Experiments on pulmonary pharmacology are often performed in laboratory animals. Investigators collect organs, samples, or record modifications in the respiratory system during anaesthesia, which might significantly influence the data attained. Indeed, it is recommended to cautiously select the anaesthetic adapted for lung studies. This experiment aimed to evaluate three selected anaesthetics usually used in laboratory animal experiments, namely, (Zolite-rumpon (Z+R) mixture, halothane (HAL), and urethane (URE)) in rats. Rats were divided into four groups, control group, kept without treatment; Zolitel and Rumpon (Z+R) at a dose of 5 and 30 mg/kg body weight intraperitoneally, respectively; HAL group (5% with oxygen supply), and URE (1.5 mg/kg body weight) by inhalation. Blood samples were collected to determine the pH of the blood, bicarbonate (HCO₃^-), carbon dioxide partial pressure (pCO2), oxygen partial pressure (pO2), alveolar oxygen (A), saturated oxygen (O₂ Sat), magnesium (Mg²⁺), calcium (Ca²⁺), sodium (Na⁺), potassium (K⁺), and chloride (Cl⁻) ions, haematocrit (Hct), and haemoglobin (Hb). The result indicates that the Z+R mixture causes slight to no differences in the measured data. At the same time, HAL and URE greatly reduced the blood pH and significantly disrupted the values of HCO3-, pCO2, pO2, A, and O2 Sat. Together, HAL and URE induced metabolic acidosis and respiratory depression. Hence, the Z+R mix is suitable for the three anaesthetics pulmonary pharmacology experiments.

Keywords

Main Subjects

Pharmacology and toxicology

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