A. E., A., Dowidar, Y., El-Nagar, H., Wafa, W., El-Ratel, I., Mousbah, A. (2022). A Review on Various Antioxidants Utilized in Bovine Semen Extenders. Journal of Applied Veterinary Sciences, 7(2), 13-24. doi: 10.21608/javs.2022.115377.1120
Abdel-khalek A. E.; Y. A. Dowidar; H. A. El-Nagar; W. M. Wafa; I. T. El-Ratel; A. M. Mousbah. "A Review on Various Antioxidants Utilized in Bovine Semen Extenders". Journal of Applied Veterinary Sciences, 7, 2, 2022, 13-24. doi: 10.21608/javs.2022.115377.1120
A. E., A., Dowidar, Y., El-Nagar, H., Wafa, W., El-Ratel, I., Mousbah, A. (2022). 'A Review on Various Antioxidants Utilized in Bovine Semen Extenders', Journal of Applied Veterinary Sciences, 7(2), pp. 13-24. doi: 10.21608/javs.2022.115377.1120
A. E., A., Dowidar, Y., El-Nagar, H., Wafa, W., El-Ratel, I., Mousbah, A. A Review on Various Antioxidants Utilized in Bovine Semen Extenders. Journal of Applied Veterinary Sciences, 2022; 7(2): 13-24. doi: 10.21608/javs.2022.115377.1120
A Review on Various Antioxidants Utilized in Bovine Semen Extenders
1Animal Production Department, Faculty of Agriculture, Mansoura University, Egypt.
2Department of Biotechnology, Faculty of Agriculture, Cairo, Al-Azhar University,
3Animal Production Research Institute, Agricultural Research Center, Dokki, Giza, Egypt
4Poultry Production Department, Faculty of Agriculture, Damietta University, Egypt
5Biotechnology department, Faculty of Agriculture, Al-azhar University, Egypt
Receive Date: 09 January 2022,
Revise Date: 09 February 2022,
Accept Date: 26 February 2022
Abstract
High-quality cryopreserved bovine semen is vital for successful artificial insemination (AI) program, which is the most inexpensive and the quickest mode of genetic improvement. Cryopreservation of sperm is an effective method in infertility management; however, it can also affect spermatozoa in post-thawed semen. Many researchers showed that supplementation of extenders with antioxidants provides a cryoprotective effect on sperm quality by minimizing the harmful impact of reactive oxygen species (ROS) and enhancing post-thaw spermatozoa. This review sheds light on the protective effects of various antioxidants considered to reduce the oxidative stress following freeze-thawing of bull semen in AI programs.
Abdel-Khalek, E.A., El-Nagar, H.A., and Omima M. Ibrahim., 2016. Effect of Leptin and Melatonin as protective additive to Tris extender on frozen semen quality of Buffalo Bulls. J. Animal and Poultry Prod., Mansoura Univ., 7(7): 225 – 231.
Ahmed, H., Jahan, S., Salman, M.M., and Ullah, F., 2019. Stimulating effects of Quercetin (QUE) in tris citric acid extender on post thaw quality and in vivo fertility of buffalo (Bubalusbubalis) bull spermatozoa.Theriogenology, 134:18–23.
Ahmed, H., Jahan, S., Ullah, H. Ullah, F. and Salman, M.M., 2020. The addition of resveratrol in tris citric acid extender ameliorates post-thaw quality parameters, antioxidant enzymes levels, and fertilizing capability of buffalo (Bubalus bubalis) bull spermatozoa. Theriogenology, 152:106–113.
Ahmed, W.M., El-Tohamy, M.M., 1997. Zinc profile in blood and semen of breeding buffalo-bull with particular emphasis on age variation and semen characteristics. In Proceedings of the 5th World Buffalo Congress, Royal Palace, Caserta, Italy, 13–16 October 1997.
Aisen, E.G., Quintana, M., Medina, V., Morillo, H., and Venturino, A., 2005. Ultramicroscopic and biochemical changes in ram spermatozoa cryopreserved with trehalose based hypertonic extenders. Cryobiology, 50: 239-249. ISSN 1981-7223
Akhter, S., Rakha, B.A., Ansari, M.S., Andrabi, S.M.H., and Ullah. N., 2011. Storage of Nili-Ravi buffalo (Bubalus bubalis) semen in skim milk extender supplemented with ascorbic acid and α-tocopherol. Pak. J. Zool., 43: 273-277. DOI: 0030-9923/2011/0002-0273 $ 8.00/0
Akhter, S., Rakha, B.A., Iqbal, R. N., and Ansari, M.S., 2014. Effect of Bovine Serum Albumin on Motility, Plasmalemma, Viability and Chromatin Integrity of Buffalo Bull Spermatozoa. Pak. J. Zool., 46(1): 115-120. DOI: 0030-9923/2014/0001-0115 $ 8.00/0
Aljady, A.M., Kamaruddin, M.Y., Jamal, A.M., and Mohd Yassin, M.Y., 2010. Biochemical study on the efficacy of Malaysian honey in infected wounds: An animal model. Med. J. Islam Acad. Sci.; 13(3): 125-132.
Amirat-Briand, L., Bencharif, D., Vera-Munoz, O., Bel Hadj Ali, H., Destrumelle, S., Desherces, S., Schmidt, E., Anton, M., and Tainturier, D., 2009. Effect of glutamine on postthaw motility of bull spermatozoa after association with LDL (low density lipoproteins) extender: preliminary results, Theriogenology, 71: 1209– 1214
Andrabi, S.M.H., Ansari, M.S., Ullah, N., and Afzal, M., 2008. Effect of nonenzymatic antioxidants in extender on post-thaw quality of buffalo (bubalus bubalis) bull spermatozoa. Pak. Vet. J., 28(4): 159-162.
Ansari, M.S., Rakha, B.A., Ullah, N., Andrabi, S.M.H., Iqbal, S., Khalid, M., and Akhter, S.,2010. Effect of exogenous glutathione in extender on the freezability of Nili-Ravi buffalo (Bubalus bubalis) bull spermatozoa. Anim. Sci. Pap. Rep., 28: 235-244. ISSN : 0860-4037
Ansari, M.S., Rakha, B.A., and Akhter, S., 2011. Effect of butylated hydroxytoluene in extender on motility, plasmalemma and viability of Sahiwal bull spermatozoa. Pak. J. Zool., 43: 311-314. DOI: 0030-9923/2011/0002-0311 $ 8.00/0
Ansari, M.S., Rakha, B.A., Ullah, N., Andrabi S.M.H., and Akhter, S., 2011a. Glutathione addition in tris-citric egg yolk extender improves the quality of cooled buffalo (Bubalus bubalis) bull semen. Pak. J. Zool., 43: 46-55. doi: 10.1016/j.repbio.2012.10.001.
Ansari, M.S., Rakha, B.A., Ullah, N., Andrabi, S.M.H., Khalid, M., and Akhter, S., 2011b. Effect of L-cysteine in tris-citric egg yolk extender on post thaw quality of Nili-Ravi buffalo (Bubalus bubalis) bull spermatozoa. Pak. J. Zool., 43: 41-47. DOI: 0030-9923/2011/0001-0041 $ 8.00/0
Ansari, M.S., Rakha, B.A., and Akhter, S., 2011d. Effect of L-cysteine in extender on post-thaw quality of Sahiwal bull semen. Anim. Sci. Pap. Rep., 29: 197-203.
Ansari, M.S., Rakha, B.A., Andrabi, S.M.H., Ullah, N., Iqbal, R., Holt, W.V. and Akhter, S., 2014. Thioglycol in extender improves the post-thaw quality of Buffalo (BUBALUS BUBALIS) Bull spermatozoa. J. Anim. Plant Sci. 24(4): 1256- 1259. ISSN:1018-7081
Asadpour, R., Jafari, R. and Nasrabadi, H.T., 2011. Influence of added vitamin C and vitamin E on frozen-thawed bovine sperm cryopreserved in citrate and Tris-based extenders. Vet. Res. Forum, 2: 37 – 44. ISSN : 2008-8140
Asadpour, R., Jafari, R., and Tayefi-Nasrabadi, H., 2011a. Effect of various levels of catalase antioxidant in semen extenders on lipid peroxidation and semen quality after the freeze-thawing bull semen. Vet. Res. Forum, 2(4): 218-221. ISSN : 2008-8140
Asadpour, R., Jafari, R., and Tayefi-Nasrabadi, H., 2012. The effect of antioxidant supplementation in semen extenders on semen quality and lipid peroxidation of chilled bull spermatozoa. Iranian J. Vet. Res., 13 (3): 246– 249.
Asghari, M., and Hasanlooe, A.R. ,2015. Interaction effects of salicylic acid and methyl jasmonate on total antioxidant content, catalase and peroxidase enzymes activity in “Serosa” strawberry fruit during storage. Scientia Horti., 197: 490–495. https://doi.org/10.1016/j.scienta.2015.10.009
Ashrafi, I., Kohram, H., and Ardabili, F.F., 2013. Antioxidative effects of melatonin on kinetics, microscopic and oxidative parameters of cryopreserved bull spermatozoa. Anim. Reprod. Sci., 139: 25–30. DOI: 10.1016/j.anireprosci.2013.03.016
Badr, M. R., Mary, G., El-Malak, A., and Hassan, M.H., 2010. Effect of Trehalose on cryopreservation, oxidative stress and DNA integrity of buffalo spermatozoa. J.Reprod. Infertil., 1(2): 50-57. ISSn: 2079-2166.
Badr, M., Rawash, Z., Azab, A., Dohreg, R., Ghattas, T., and Fathi, M., 2021. Spirulina platensis extract addition to semen extender enhances cryotolerance and fertilizing potentials of buffalo bull spermatozoa. Anim. Reprod., 18(2): 1-12. https://doi.org/10.1590/1984-3143-AR2020-0520
BADR, M.R., AZAB, A.M.S., and RAWASH, Z.M., 2014. Effect of Trehalose, Cysteine and Hypotaurine on Buffalo Bull sperm freezaBility, ultrastructure changes and fertilizing poteNtials. Assiut Vet. Med. J., 60 (142).
Ball, B.A., Medina., V., Gravance, C.G., and Baumber, J., 2001. Effect of antioxidants on preservation of motility, viability and acrosomal integrity of Equine spermatozoa during storage at 5oC. Theriogenology, 56: 577-589. DOI: 10.1016/s0093-691x(01)00590-8
Bansal, A.K., and Bilaspuri, G.S., 2008. Effect of manganese on bovine sperm motility, viability, and lipid peroxidation in vitro. Anim. Reprod., 5(3/4): 90-96.
Bath, M.L. 2010. Inhibition of in vitro fertilizing capacity of cryopreserved mouse sperm by factors released by damaged sperm, and stimulation by glutathione. PLoS One, 24: e9387.
https://doi.org/10.1371/journal.pone.0009387
Beconi, M.T., Francia, C.R., Mora, N.G., and Affranchino., 1993. Effect of natural antioxidant of frozen bovine semen preservation. Therio., 40:841-851. https://doi.org/10.1016/0093-691X(93)90219-U
Beheshti, R., Aiden Asadi, A., and Naser Maheri-Sis, N., 2011. The effect of vitamin E on post-thawed buffalo bull sperm parameters. J. American Sci., 7(6): 227-231. ISSN: 1545-1003). http://www.americanscience.org
Bilodeau, J.F., Blanchette, S., Gagnon, I.C., and Sirard, M.A., 2001. Thiols prevent H2O2- mediated loss of sperm motility in cryopreserved bull semen, Theriogenology, 56: 275–286. DOI: 10.1016/s0093-691x(01)00562-3
Boots, A.W., Haenen, G.R., and Bast, A., 2008. Health effects of quercetin: from antioxidant to nutraceutical. Eur. J. Pharmacol., 585: 325–337. DOI: 10.1016/j.ejphar.2008.03.008
Bucak, M.N., Ataman, M.B., Baspinar, N., Uysal, O., Taspinar, M., Bilgili, A., Ozturk, C., Gungor, S., EInanc, M., and Akal, E., 2014. Lycopene and resveratrol improve postthaw bull sperm parameters: sperm motility, mitochondrial activity and DNA integrity. Andro., 47: 542-545. doi: 10.1111/and.12301
Bucak, M.N., Baspinar, N., Tuncer, P.B., Coyan, K., Sankozan, S., Akalin, P.P., and Kucukgunay, S. , 2012. Effects of curcumin and dithioerythritol on frozen-thawed bovine semen. Andro., 44: 102–109. doi: 10.1111/j.1439-0272.2010.01146.x
Bucak, M.N., Tuncer, P.B., Sarıozkan, S., Pınar, N.B., Pınar, M.T., Coyan, K., Bilgili, A., Akalın, P.P., and Buyukleblebici, S., 2010. Effects of antioxidants on post-thawed bovine sperm and oxidative stress parameters: Antioxidants protect DNA integrity against cryodamage. Cryobiology, 61: 248–253. doi:10.1016/j.cryobiol.2010.09.001
Chhillar, S., Singh, V.K., Kumar, R., and Atreja S.K., 2012. Effects of Taurine or Trehalose supplementation on functional competence of cryopreserved Karan Fries semen. Anim. Reprod. Sci., 135 (1-4):1-7.
Chikhaliya P.S., Ahlawat, A.R., Singh, V.K., Vijyeta, H.P., Odedra, M.D., and Vala, K.B., 2018. Effect of Different Concentrations of Taurine on Certain Biochemical Parameters During Cryopreservation of Gir Bull Semen. Int. J. Curr. Microbiol. App. Sci., 7(7): 1441-1447. https://doi.org/10.20546/ijcmas.2018.707.171
Chuawongboon, P., Sirisathien, S., Pongpeng, J. Sakhong D., nagai, T., and Vongpralub, T., 2017. Effects of supplementation of iodixanol to semen extender on quality and fertilization ability of frozen–thawed Thai native bull sperm. Anim. Sci. J., 88: 1311–1320. DOI: 10.1111/asj.12798
Collodel, G., Federico, M.G., Geminiani, M., Martini, S., Bonechi, C., Rossi, C., Figura, N., and Moretti, E., 2011. Effect of transresveratrol on induced oxidative stress in human sperm and in rat germinal cells. Reprod. Toxicol. 31:239–246. DOI: 10.1016/j.reprotox.2010.11.010
Coyan, K., Bas¸ pınar, N., Bucak, M.N., Akalın, P.P., Ataman, M.B., Omur, A.D., Gungor, S.¸ Kucukgunay, S., Ozkalp, B., and Sarıozkan, S., 2010. Influence of methionine and dithioerythritol on sperm motility, lipid peroxidation and antioxidant capacities during liquid storage of ram semen. Res. Vet. Sci. 89:426–431. DOI: 10.1016/j.rvsc.2010.03.025
Daghigh-Kia H., Olfati-Karaji, R., Hoseinkhani, A., and Ashrafi, I., 2014. Effect of rosemary (Rosmarinus officinalis) extracts and glutathione antioxidants on bull semen quality after cryopreservation. Span. J. of Agri. Res., 12(1): 98-105. http://dx.doi.org/10.5424/sjar/2014121-4486
Daghigh-Kia, H., Farhadi, R., Ashrafi, I., and Mehdipour, M., 2016.Anti‐oxidative Effects of Ethanol Extract of Origanum vulgare on Kinetics, Microscopic and Oxidative Parameters of Cryopreserved Holstein Bull Spermatozoa. Iran. J. of Appl. Anim. Sci., 6(4): 783-789.
Dorostkar K., Alavi-Shoushtari, S.M., and Mokarizadeh, A., 2012. Effects of in vitro selenium addition to the semen extender on the spermatozoa characteristics before and after freezing in water buffaloes (Bubalus bubalis). Vet. Res. Forum, 3 (4): 263 – 268 PMCID: PMC4313046, PMID: 25653769
Dorostkar, K., Alavi Shoushtari, S.M., and Khaki, A., 2014. Effects of in vitro zinc sulphate additive to the semen extender on water buffalo (bubalusbubalis) spermatozoa before and after freezing. Int. J. Ferti. Steril., 8(3): 325-332. PMCID: PMC4221520, PMID: 25379162
Dowidar, Y.A., El-Nagar, H.A, El Refy, A.M. and Mousbah, A.M. 2018. Cryopreservation and Quality Assessment of Buffalo Bull (Bubalus bubalis) Semen Using New Moringa Extender and Antioxidant Co-Q10. J. Anim. Poult. Prod., Mansoura Univ., 9: 375 – 381. DOI: 10.21608/JAPPMU.2018.41144
El-Nagar, H.A., EL Refy, A.M., A. M. and Mousbah, A.M., 2019. Freezing and Fertilizing abilities of Summer Semen of Egyptian buffalo (Bubalus bubalis) Bulls Using Moring Extract as Antibiotic or as a New Promising Extender. J. Anim. and Poult. Prod., Mansoura Univ., 10 (3): 73 – 81. DOI: 10.21608/jappmu.2019.40527
El-Sheshtawy, R.I., and El-Nattat, W.S., 2017. Effect of Diospyros kaki enriched extender on cattle bull sperm parameters and conception rate. Asian Pacific Journal of Reproduction; 6(3): 128-132. DOI: 10.12980/apjr.6.20170306
El-Sheshtawy, R.I., and El-Nattat, W.S., 2017a. Impact of silymarin enriched semen extender on bull sperm preservability. Asian Pacific Journal of Reproduction; 6(2): 81-84. DOI: 10.12980/apjr.6.20170206
El-Sheshtawy, R.I., and El-Nattat, W.S., 2018. Effect of tris-extender supplemented with various concentrations of strawberry (Fragaria spp.) on bull semen preservability. Asia. Paci. J. of Reprod., 7(2): 93-96. doi: 10.4103/2305-0500.228019
El-Sheshtawy, R.I., El-Nattat, W.S., Ali, A.H., and Sabra, H.A., 2014a. The Effect of Phoenix dactylifera pollen grains tris-infusion on semen preservability of local bull breeds. Glob. Vet., 13(5): 728-732. DOI: 10.5829/idosi.gv.2014.13.05.86145
El-Sheshtawy, R.I., El-Nattat, W.S., and Ali, G.A., 2016. Preservability of Buffalo semen using tris-extender enriched with different concentrations of Strawberry. Int. J. of Chem. Tech. Res., 9(6): 198-202.
El-Sheshtawy, R.I., El-Nattat, W.S., Sabra, H.A., and Ali, A.H., 2014b. Effect of Honey Solution on Semen Preservability of Local Breeds of Cattle Bulls. Wld. Appl. Sci. J., 32(10): 2076-2078.DOI: 10.5829/idosi.wasj.2014.32.10.911
El-Sheshtawy,R.I., El-Nattat, W.S., Shalaby, S.I.A., Shahba, M.I., and Al-Se'dawy, I.E., 2016a. Chilled and post-thawed semen characteristics of buffalo semen diluted in tris extender enriched with date palm pollen grains (TPG). Asia. Paci., J. of Reprod., 5(3): 252–255. DOI : 10.1016/j.apjr.2016.04.001
El-Sisy, G.A., El-Nattat, W.S., and El-Sheshtawy, R.I., 2008. Effectof superoxide dismutase and catalase on viability of cryopreserved buffalo spermatozoa. Global Veterinaria, 2(2): 65–61. ISSN: 1992-6197
El-Tantawy, W.H. 2016. Antioxidant effects of Spirulina supplement against lead acetate-induced hepatic injury in rats. J. Tradit. Complement. Med., 6: 327–331. DOI: 10.1016/j.jtcme.2015.02.001
Esteves, S.C., Spaine, D.M., and Cedenho, A.P., 2007. Effects of pentoxyfylline treatment before
freezing on motility, viability and acrosome status of poor quality human spermatozoa cryopreserved by the liquid nitrogen vapor method. Brazi. J. Med. Biol. Res., 40. DOI: 10.1590/s0100-879x2006005000118
Fernandez-Santos, M.R., Dominguez-Rebolledo, A.E., Esteso, M.C., Garde, J.J., and Martinez-Pastor, F., 2009. Catalase supplementation on thawed bull spermatozoa abolishes the detrimental effect of oxidative stress on motility and DNA integrity. Int. J. Androl., 32: 353–359. DOI: 10.1111/j.1365-2605.2008.00871.x
Fu, J., Yang, Q., and Li, Y., 2018. A mechanism by which Astragalus polysaccharide protects against ROS toxicity through inhibiting the protein dephosphorylation of boar sperm preserved at 4 degrees C. J.Cell Physiol., 233, 5267–5280. DOI: 10.1002/jcp.26321
FUKUZAWA, K., SAITOH, Y., AKAI, K., KOGURE, K., UENO, S., TOKUMURA, A., OTAGIRI, M., and SHIBATA, A., 2005. Antioxidant effect of bovine serum albumin on membrane lipid peroxidation inducedby iron chelate and superoxide. Biochim. biophys. Acta., 1668: 145-155. DOI: 10.1016/j.bbamem.2004.12.006
Garg, A., Kumaresan, A. and Ansari, M.R., 2009. Effect of hydrogen peroxide (H2O2) on fresh and cryopreserved buffalo sperm functions during incubation at 37oC in vitro. Reprod. Dome. Anim., 44: 907-912. DOI: 10.1111/j.1439-0531.2008.01115.x
Ghosh, I., Bharadwaj, A., and Datta, K., 2002. Reduction in the level of hyaluronan binding protein (HABP1) is associated with loss of sperm motility. J. Reprod. Immunol. 53:45–54. DOI: 10.1016/s0165-0378(01)00095-x
Gohar, A., Khan, H., Yousef, M.S. Ahmed, I., Ali, Q., Khan, M., Khan, D., Hayat, Y., Ali, F., Ahmed, I., Salim, M., and Ullah, F., 2014. Assessment of Alpha Lipoic Acid Inclusion In Semen Extenders On Cryopreservation Of Nili-Ravi Buffalo Bull Spermatozoa. Life Sci. J., 11 (9s).45-50. ISSn: 1097-8135
Granaci, V., 2007. Contributions on the study of the cryoresistance increase of the bull semen material. Bulletin of University of Agricultural Sciences and VeterinaryMedicine Cluj-Napoca. Anim. Sci. Biotechnol.,63/64:387–391.
Guerin, P., Guillaud, J. and, Menezo, Y., 1995.Hypotaurine in spermatozoa and genetalsecretions and its production by oviductepithelial cells in vitro. Hum. Reprod., 10:866-872. DOI: 10.1093/oxfordjournals.humrep.a136052
GUNGOR, S., AKSOY, A., YENİ, D., AVDATEK, F., OZTURK, C., ATAMAN, M.B., COYAN, K., BUCAK, M.N., BAŞPINAR, N., and AKALIN, P.P., 2016. Combination of Cysteamine and Lipoic Acid Improves the Post-Thawed Bull Sperm Parameters. Kocatepe Vet. J., 9(2):88-96. DOI: 10.5578/kvj.23105
Hu, J.H., Zan, L.S., Zhao, X.L., Li, Q.W., Jiang, Z.L., Li, Y.K. and Li, X., 2010. Effects of trehalose supplementation on semen quality and oxidative stress variables in frozen-thawed bovine semen. J. Anim. Sci., 88: 1657-1662. DOI: 10.2527/jas.2009-2335
Hussein, Y.S., Shaba, M.I., and El-Sheshtawy, R.I., 2019. Lactoferrin addition to Tris-extender reduces the detrimental effects of cryopreservation on Egyptian buffalo semen. Mid. East J. of App. Sci., 9(4): 1057-1062. DOI: 10.36632/mejas/2019.9.4.21
Ibrahim, S.F., Jaffar, F.H.F., Khairul Osman K., Mohamed S.F.S., Nang C.F., Ismail N.H., and Ismail M.I., 2011. Bull spermatozoa Motility: Optimization of Coenzyme Q10 and Alpha-Lipoic Acid Concetration. IIOABJ., 2(5): 8-13.
Jaiswal, B.S., Das, K., Saha, S., Dungdung, S.R., and Majumder, G.C., 2010. Purification and characterization of a motility initiating protein from caprine epididymal plasma. J. Cell Physiol., 222: 254–263. DOI: 10.1002/jcp.21947
Kadirvel, G., Kumar, S., Ghosh, S.K., and Perumal, P., 2104. Activity of antioxidative enzymes in fresh and frozen thawed buffalo (Bubalus bubalis) spermatozoa in relation to lipid peroxidation and semen quality. Asia. Paci. J. of Reprod., 3(3): 210-17.
Khaki, A., Batavani, R.A., and Najafi,G., 2013. The in vitro effect of leptin on semen quality of water buffalo (Bubalus bubalis) bulls. Vet. Res. Forum, 4(1): 7-12. Corpus ID: 2940273
Khan, H., Khan, M., Qureshi, M.S., Ahmad, S., Gohar, A., Ullah, H., Ullah, F., Hussain, A., Khatri, P., Shah,S.S.A., Rehman, H., and Khan, A., 2017. Effect of Green Tea Extract (Camellia sinensis) on Fertility Indicators of Post-Thawed Bull Spermatozoa. Pak. J. Zool., 49(4): 1243-1249. DOI:http://dx.doi.org/10.17582/journal.pjz/2017.49.4.1243.1249
Lange Consiglio, A., Dell'Aquila, M.E., Fiandanese, N., Ambruosi, B., Cho, Y.S., and Bosi, G., 2009. Effects of leptin on in vitro maturation, fertilization and embryonic cleavage after ICSI and early developmental expression of leptin (Ob) and leptin receptor (ObR) proteins in the horse. Reprod. Biol. Endocrinol.,7:113. doi: 10.1186/1477-7827-7-113
Lapointe, S., Ahmad, I., Buhr, M.M., and Sirard, M.A., 1996. Modulation of post-thaw motility, survival, calcium uptake and fertility of bovine sperm by magnesium and manganese. J. Dairy. Sci., 79: 2163-2169. DOI: 10.3168/jds.S0022-0302(96)76592-X
Longobardi, V., Valle, G., Iannaccone, F. Calabria, A., Vuolo, G.D., Damiano, S., Ciarcia, R., and Gasparrini, B., 2021. Effects of the antioxidant crocin on frozen-thawed buffalo (Bubalusbubalis) sperm, Ital. J. of Anim. Sci., 20(1): 2095-2101. DOI: 10.1080/1828051X.2021.1997653
Lovell, M.A., Xie, C., and Xiong, S., Markesberywe., 2003. Protection against amyloid beta peptide and iron/hydrogen peroxide toxicity by alpha lipoic acid. J. Alzheimers Dis., 5: 229-239. DOI: 10.3233/jad-2003-5306
Luangpirom, A., Junaimuang, T., Kourchampa, W., Somsapt, P., and Sritragool, O., 2013. Protective effect of pomegranate (Punica granatum Linn.) juice against hepatotoxicity and testicular toxicity induced by ethanol in mice. Anim. Biol. Anim. Husb. Int. J. Bioflux Soc., 5(1): 87-93. DOI: 10.1186/1472-6882-14-164
Longobardi, V., Zullo, G., Salzano, A., De Canditiis, C., Cammarano, A., De Luise, L., Puzio MV, Neglia, G., and Gasparrini, B. 2017. Resveratrol prevents capacitationlike changes and improves in vitro fertilizing capability of buffalo frozenthawed sperm. Theriogenology, 88: 1–8. DOI: 10.1016/j.theriogenology.2016.09.046
Mansouri, A., Embarek, G., Kokkalou, E., and Kefalas, P., 2005. Phenolic profile and antioxidant activity of the Algerian ripe date palm fruit (Phoenix dactylifera). Food Chem., 89: 411-420. DOI:10.1016/j.foodchem.2004.02.051
Motemani, M., Chamani, M., Sharafi, M., and Masoudi, R., 2017. Alpha-tocopherol improves frozen-thawed sperm quality by reducing hydrogen peroxide during cryopreservation of bull semen. Span. J., Agri. Res, 15(1): 7.
Marqui, F.N., Martins, A., da Cruz, T.E., Berton, T.I.U. Dell’Aqua1, C.d.F., Júnior, J.A.D., and Oba, E., 2018. Addition of iodixanol in bull freezing extender improves the sperm membranes integrity. Animal Reprod. Sci., 194: 26–27. https://doi.org/10.1016/j.anireprosci.2018.04.058
Martins, H.S., da Silva, G.C., Cortes, S.F., Paes, F.O., Martins Filho, O.A., Araujo, M., Stahlberg, R., and Lagares, M.A., 2018. Lactoferrin increases sperm membrane functionality of frozen equine semen. Reprod. Domest. Anim., 53(3): 617-623. DOI: 10.1111/rda.13148
Maxwell, D.T., Jacobson, J.D., King, A., and Chan, P.J., 2002. Effect of pentoxifylline on tumor suppressor and protooncogene apoptosis in sperm. J. Assist. Reprod. Genet., 19(6):279-283. doi: 10.1023/A:1015725230011
Merton, J.S., Knijn, H.M., Flapper, H., Dotinga, F., Roelen, B.A., Vos, P.L., and Mullaart, E., 2013. Cysteamine supplementation during in vitro maturation of slaughterhouse- and opu-derived bovine oocytes improves embryonic development without affecting cryotolerance, pregnancy rate, and calf characteristics. Theriogenology, 80: 365–371. DOI: 10.1016/j.theriogenology.2013.04.025
Mirnamniha, M., Faroughi, F., Tahmasbpour, E. Ebrahimi, P. and Harchegani, A.b., 2019. An overview on role of some trace elements in human reproductive health, sperm function and fertilization process. Rev. Environ. Health, 34: 339–348. DOI: 10.1515/reveh-2019-0008
Mizera, A., Kuczaj, M., and Szul, A., 2019. Impact of the Spirulina maxima extract addition to semen extender on bovine sperm quality. Ital. J. Anim. Sci.18(1): 601-7.
https://doi.org/10.1080/1828051X.2018.1548914
Muzafer, A.B., Rao, M.M., Sanjay, A., Pandey, A.K., Verma, P.K., and Sultana, M., 2012. Effect of antioxidant supplementation on post-thaw sperm characteristics, membrane integrity, migration capability and lipid peroxidation in bull semen. Indian. J. Anim. Sci., 82: 457-460. ISSN: 0367-8318
Nang, C.F., Osman, K., Budin, S.B., Ismail, M.I., Jaffar, F.H.F., Mohamad, S.F.S., and Ibrahim, S.F., 2011. Bovine serum albumin: survival and osmolarity effect in bovine spermatozoa stored above freezing point. Andrologia, 44: 447–453. doi: 10.1111/j.1439-0272.2011.01203.x
Nasiri, A.H., Towhidi, A., and Zeinoaldini, S., 2012. Combined effect of DHA and α-tocopherol supplementation during bull semen cryopreservation on sperm characteristics and fatty acid composition. Andrologia 44: 550-555. DOI: 10.1111/j.1439-0272.2011.01225.x
Nguyen, V.H., Le, M.N., Nguyen, H.B., Ha, K.O., Pham, T.H.V., Nguyen, T.H., Dao, N.S.H., Nguyen, V.G., Nguyen, D.L., and Trinh, N.T., 2021. Propyl Gallate. Molbank 2021, M1201. https://doi.org/10.3390/M1201 Osman, K., Ibrahim, S.F., Zakaria, N.A., Ismail, M.I., Abd Karim, A.A., and Ismail, Z., 2012. Effect of Alpha lipoic on cattle sperm kinetics using computer assisted semen analysis. Res. J. Bio. Sci., 7 (2): 73-77. DOI: 10.3923/rjbsci.2012.73.77
Pankaj, P.K, Raina, S., Roy, B., Mohanty, T.K., and Mishra, A., 2009. Effect of Antioxidant Preservative on Cold Protection Ability of Low Grade Riverine Buffalo (Bubalus bubalis) Bull Spermatozoa, Asian-Aust. J. Anim. Sci., 22, 5 : 626 – 635. https://doi.org/10.5713/ajas.2009.70267
Patra R.C., Swarup, D., and Dwivedi, S.K., 2001. Antioxidant effects of alpha-tocopherol, ascorbic acid and L-methionine on lead induced-oxidative stress to the liver, kidney and brain in rats. Toxicology, 162: 81–88. DOI: 10.1016/s0300-483x(01)00345-6
Perreault, S.G., and Roger, B.J. 1992. Relationship between fertilizing ability and cAMP in human spermatozoa. J. Andrology, 13: 396-401. DOI: 10.1046/j.1341-8076.2003.00119_2.x
Peruma, P., Chamuah, J.K., and Rajkhowa, C., 2013. Effect of catalase on the liquid storage of mithun (Bos frontalis) semen. Asian Pac. J. Reprod., 2: 209–214. doi: 10.1016/S2305-0500(13)60148-7
Peruma, P., Selvaraju, S., Barik, A.K., Mohanty, D.N., Das, S., and Mishra, P.C., 2011. Role of reduced glutathione in improving post-thawed frozen seminal characters of poor freezable Jersey crossbred bull semen. Indian J. Anim. Sci., 81 (8): 807- 810.
Perumal, P., 2014. Effect of Superoxide Dismutase on Semen Parameters and Antioxidant Enzyme Activities of Liquid Stored (5∘C) Mithun (Bos frontalis) Semen. J. of Animals, 1-9.
Pisoschi, A.M., and Pop, A., 2015. The role of antioxidants in the chemistry of oxidative stress: A review. Eur. J. Med. Chem., 97: 55–74. DOI: 10.1016/j.ejmech.2015.04.040
Rao, M.V., and Gangadharan, B., 2008. Antioxidative potential of melatonin against mercury induced intoxication in spermatozoa in vitro. Toxicology in Vitro, 22: 935–942. DOI: 10.1016/j.tiv.2008.01.014
Reddy, N.S.S., Mohanarao, G.J., and Atreja, S.K., 2010. Effects of adding taurine and trehalose to a tris-based egg yolk extender on buffalo Bubalus bubalis sperm quality following cryopreservation. Anim. Reprod. Sci., 119: 183- 190. doi:10.1016/j.anireprosci.2010.01.012
Reyes-Moreno, C., Gagnon, A., Sullivan, R., and Sirard, M.A., 2000. Addition of specific metabolites to bovine epididymal cell culture medium enhances survival and motility of cryopreserved sperm. J. Androl., 21: 876–886. PMID: 11105914
Rizk, B., Fountain, S., Avery, S. Palmer, C., Blayney, M., Macnamee, M., Mills C., and Brinsden, P., 2005. Successful use of pentoxifylline in malefactor infertility and previous failure of in vitro fertilization: A prospective randomized study. J. Assisted Reprodn. Gen., 12(10): 710-714.
ROCA, J., GILT, M.A., HERNANDEZ, M., PARRILIA, I., VAZQUEZ, J.M., and MARTINEZ, E.M., 2004. Survival and fertility of boar spermatozoa after freeze-thawing in extender supplemented with butylated hydroxytoluene. J. Androl., 25: 397–405. DOI: 10.1002/j.1939-4640.2004.tb02806.x
ROCHE, M., RONDEAU, P., SINGH, N.R., TARNUS, E., and BOURDON, E., 2008. The antioxidant properties of serum albumin. FEBS Lett., 582:1783-1787. DOI: 10.1016/j.febslet.2008.04.057
Sanchez-Gutierrez, M., Garcıa-Montalvo, E.A., Izquierdo-Vega, J.A., and Del Razo, L.M., 2008. Effect of dietary selenium deficiency on the in vitro fertilizing ability of mice spermatozoa. Cell Biol. Toxicol., 24:321-329. DOI 10.1007/s10565-007-9044-8
SHOAE, A., and ZAMIRI, M.G., 2008. Effect of butylated hydroxytoluene on bull spermatozoa frozen in egg yolk-citrate extender. Anim. Reprod. Sci., 104: 414-418. DOI: 10.1016/j.anireprosci.2007.07.009
SarıOzkan S, Tuncer PB, BUyükleblebici S, Bucak MN, Cantürk F, and Eken A., 2015. Antioxidative effects of cysteamine, hyaluronan and fetuin on post-thaw semen quality, DNA integrity and oxidative stress parameters in the Brown Swiss bull. Andrologia , 47: 138–147. doi: 10.1111/and.12236
Sarıozkan, S., Bucak, M.N., Tuncer, P.B., Buyukleblebici, S., and Canturk, F., 2014. Influence of various antioxidants added to TCM-199 on post-thaw bovine sperm parameters, DNA integrity and fertilizing ability. Cryobiology, 68: 129–133. http://dx.doi.org/10.1016/j.cryobiol.2014.01.007
Sariozkan, S., Tuncer, P.B., Bucak, M.N., and Ulutas, P.A., 2009. Influence of various antioxidants on microscopic oxidative stress indicators and fertilizing ability of frozen-thawed bull semen. ACTA VET. BRNO, 78: 463-469. https://doi.org/10.2754/avb200978030463
Shah, S.A.H., Andrabi, S.M.H., and Qureshi, I.Z., 2016. Effect of equilibration times, freezing and thawing rates on post-thaw quality of buffalo (Bubalus bubalis) bull spermatozoa. Andrology, 4: 972–976. DOI: 10.1111/andr.12214
Shukla, M.K., and Misra, A.K., 2005. Effect of antioxidants alpha - tocopherol, ascorbic acid and n-propyl gallate on Murrah semen cryopreservation. Buffalo J., 21: 27–38.
Shukla, M.K., and Misra, A.K., 2007. Effect of Bradykinin on Murrah buffalo (Bubalus bubalis) semen cryopreservation. Anim. Reprod. Sci., 97: 175–179. doi:10.1016/j.anireprosci.2006.02.015
Siems,W.E., Maul, B., Wieshner, B., Becker, M., Walther, T., Rothe, L., and Winkler, A., 2003. Effect of Kinins on mammalian spermatozoa and the impact of peptidolytic enzymes. Andrologia, 35: 44–54. DOI: 10.1046/j.1439-0272.2003.00536.x
Singla, R.K., and Giliyaru, V.B., 2011. Crocin: an overview indo glob. J Pharm Sci., 1(4):281–286. ISSN 2249- 1023
Somlev, B., and Subev, M., 1998. Effect of kininase II inhibitors on bradykinin-stimulated bovine sperm motility. Theriogenology, 50: 651–65. DOI: 10.1016/s0093-691x(98)00169-1
Sonmez, M., Yuce, A., and Turk, G., 2007. The protective effects of melatonin and Vitamin E on antioxidant enzyme activities and epididymal sperm characteristics of homocysteine treated male rats. Reprod.Toxico., 23: 226–231. DOI: 10.1016/j.reprotox.2006.11.003
Succu, S., Berlinguer, F., Pasciu, V., Satta, V., Giovanni G Leoni, G.G. and Naitana, S., 2011. Melatonin protects ram spermatozoa from cryopreservation injuries in a dose-dependent manner. Journalof Pineal Research 50: 310–318. Doi:10.1111/j.1600-079X.2010.00843.x
Swain, D.K., Kudu, A.K., Rath, A., and Mohapatra, A.P.K., 2009. Effect of addition of antioxidants on post-thaw semen quality of cryopreserved bull sperm. Indian J.Anim. Sci., 79(5): 470-472.
Tang, D., Chen, K., Huang, L., and Li, J., 2017. Pharmacokinetic properties and drug interactions of apigenin, a natural flavone. Expert Opin. Drug Metab. Toxicol., 13: 323–330. DOI: 10.1080/17425255.2017.1251903
Towhidi, A., and Parks, J.E., 2012. Effect of n-3 fatty acids and α-tocopherol on post-thaw parameters and fatty acid composition of bovine sperm. J. Assist. Reprod. Genetic, 29: 1051-1056. doi: 10.1007/s10815-012-9834-7
Tuncer, P.B., Bucak, M.N., Buyukleblebici, S., Sarıözkan, S., Yeni, D., Eken, A., Akalın, P.P., Kinet, H., Avdatek, F., Fidan, A.F., and Gundogan, M., 2011.The effect of cysteine and glutathione on sperm and oxidative stress parameters of post-thawed bull semen. Cryobiol., 61: 303-307. DOI: 10.1016/j.cryobiol.2010.09.009
Tvrdá E., Luká, N., Jambor, T., Lukáčová, J., Hashim, F., and Massányi, P., 2016. In vitro supplementation of lycopene to bovine spermatozoa: effects on motility, viability and superoxide production. Anim. Sci. Papers and Reports 34(4): 319-328.
Tvrda, E., Lukac, N., Lukacova, J., Knazicka, Z., and Massanyi, P., 2013. Stimulating and protective effects of vitamin e on bovine spermatozoa. J. Microbiol.Biotechnol. Food Sci., 2: 1386-1395.
Uysal, O., and Bucak, M.N., 2007. Effects of oxidized glutathione, bovine serum albumin, cysteine and lycopene on the quality of frozen-thawed ram semen. Acta Veterinaria Brno, 76:383-390. DOI:10.2754/avb200776030383
Uysal, O., Buck, M.N., Yavas, I., and Varisli, O., 2007. Effect of various antioxidants on the quality of frozen thawed bull semen. J. Anim. Vet. Ad., 6(12): 1362-1366. ISSn: 1680-5593.
Wafa, W.M., El-Nagar, H.A., Hussein Y.S., and Saeed, A.M., 2021. Effect of Different Antioxidant Sources Added to Buffalo Semen Extender During Cryopreservation on Freezability and Fertility of Buffalo Spermatozoa. J. Anim. Health Prod. 9(3): 222-228. DOI:10.17582/journal.jahp/2021/9.3.222.228
Wang, H., Lu, P., Yuan, C., Zhao, J., Liu, H., Lu, W., Wang, J. 2021. Effects of Apigenin and Astragalus Polysaccharide on the Cryopreservation of Bull Semen. Animals, 11: 1506. doi.org/10.3390/ani11061506
Watanabe, H, and Fukui, Y., 2006. Effects of dithiothreitol and boar on pronuclear formation and embryonic development following intracytoplasmic sperm injection in pigs. Theriogenol., 65:528–539. https://doi.org/10.1016/j.theriogenology.2005.04.031
Yunes, R., Fernández, P., Doncel, G.F., and Acosta, A.A., 2005. Cyclic nucleotide phosphodiesterase inhibition increases tyrosine phosphorylation and hyper motility in normal and pathological human spermatozoa. Biocell, 29(3):287-293. PMID: 16524250