Histological and Clinical Finding of Systemic Melatonin on Wound Healing after Tooth Extraction in Rabbits.

Taqa, Ghada A.; Albanna, Shahad S.

doi:10.21608/javs.2021.98538.1107

Histological and Clinical Finding of Systemic Melatonin on Wound Healing after Tooth Extraction in Rabbits.

Document Type : Original Article

Authors

Department of Dental Basic Science, College of Dentistry, University of Mosul, Mosul, Iraq.

10.21608/javs.2021.98538.1107

Abstract

This study aimed to evaluate the effects of systemic melatonin on wound healing after tooth extraction.This study was conducted on twenty healthy adult male New Zealand albino rabbits. Tooth extraction was done on all rabbits, and then animals were divided into two major groups. Control group (n=10): received no treatment. Melatonin treated group (n=10): received melatonin orally (1mg\kg) dissolved in distilled water; gave it to the rabbits once daily for a determined period. On days seventh and fourteenth, all animals in each group were sacrificed. The wound area site undergoes histological examination to compare the healing among the control group and melatonin treated0group. The present study demonstrated that melatonin treated group found to accelerate wound healing manifested by a score of angiogenesis (1.6 ± 0.54), granulation tissue (2 ± 0.70) and reepithelization (1.2 ± 0.44) while a decrease in inflammatory cells (0 ± 0.00) in comparison to control group at 7^th and (2 ± 0.70),( 2.2 ± 0.44),(2.6 ± 0.54) respectively and decrease in inflammation(0 ± 0.00) at the 14^th day.In this study, we found that systemic melatonin accelerates socket wound healing after tooth extraction.
.

Keywords

Main Subjects

Pharmacology and toxicology

References

ALI, Z., and BHASKARr, S. B., 2016. Basic statistical tools in research and data analysis. Indian journal of anaesthesia, 60(9), 662.

https://dx.doi.org/10.4103%2F0019-5049.190623.

AYRANCI, B., KALENDER, A. M., SEVIMLI, R., KORKMAZ, M. F., and BILAL, O., 2016. The effect of tramadol HCL and paracetamol on fracture healing in rat tibia model.

https://dx.doi.org/10.5455/medscience.2016.05.8527.

BARRIGA, C., MARTIN, M. I., TABLA, R., ORTEGA, E., and RODRIGUEZ, A. B., 2001. Circadian rhythm of melatonin, corticosterone and phagocytosis: effect of stress. Journal of Pineal Research, 30(3), 180–187.https://doi.org/10.1034/j.1600-079X.2001.300307.x.

CHEN, K., TONG, C., CONG, P., LIU, Y., SHI, X., LIU, X., ZHANG, J., ZOU, R., XIAO, K., and NI, Y., 2021. Injectable melatonin-loaded carboxymethyl chitosan (CMCS)-based hydrogel accelerates wound healing by reducing inflammation and promoting angiogenesis and collagen deposition. Journal of Materials Science & Technology, 63, 236–245. https://doi.org/10.1016/j.jmst.2020.06.001.

DE RENSIS, F., MORINI, G., GARCIA-ISPIERTO, I., and López-Gatius, F., 2020. To what extent does photoperiod affect cattle reproduction? Clinical perspectives of melatonin administration. Annals of Animal Science Science, 2020, vol. 20, núm. 3, p. 797-809.https://doi.org/10.1034/j.1600-079X.2001.300307.x.

FARINA, R., and TROMBELLI, L., 2011. Wound healing of extraction sockets. Endodontic Topics, 25(1), 16–43. https://doi.org/10.1111/etp.12016.

GUPTA, A., and KUMAR, P., 2015. Assessment of the histological state of the healing wound. Plastic and Aesthetic Research, 2, 239–242.

https://doi.org/10.4103/2347-9264.158862.

HARDELAND, R. 2017. Melatonin and the pathologies of weakened or dysregulated circadian oscillators. Journal of Pineal Research, 62(1), e12377.

https://doi.org/10.1111/jpi.12377.

IMENSHAHIDI, M., KARIMIO, G., and HOSSENZAHED, H., 2020. Effects of melatonin on cardiovascular risk factors and metabolic syndrome: a comprehensive review. Naunyn-Schmiedeberg’s Archives of Pharmacology, 393(4), 521–536. https://doi.org/10.1007/s00210-020-01822-4.

JIN, H., ZHANG, Z., WANG, C., TANG, Q., WANG, J., BAI, X., WANG, Q., NISAR, M., TIAN, N., and WANG, Q., 2018. Melatonin protects endothelial progenitor cells against AGE-induced apoptosis via autophagy flux stimulation and promotes wound healing in diabetic mice. Experimental & Molecular Medicine, 50(11), 1–15.

https://doi.org/10.1038/s12276-018-0177-z.

KACZMAREK-SZCZEPANSKA, B., OSTROWSKA, J., KOZLOWSKA, J., SZOTA, Z., BROZENA, A. A., DREIER, R., REITER, R. J., SSLOMINSKI, A. T., STEINBRINK, K., and KLESZCZYNSKI, K., 2021. Evaluation of Polymeric Matrix Loaded with Melatonin for Wound Dressing. International Journal of Molecular Sciences, 22(11), 5658.

https://doi.org/10.3390/ijms22115658.

KHOSWANTO, C. 2019. A new technique for research on wound healing through extraction of mandibular lower incisors in Wistar rats. European Journal of Dentistry, 13(02), 235–237. https://dx.doi.org/10.1055%2Fs-0039-1694312.

KULKARENI, S., MEER, M., and GEORGE, R., 2019. Efficacy of photobiomodulation on accelerating bone healing after tooth extraction: a systematic review. Lasers in Medical Science, 34(4), 685–692. https://doi.org/10.1007/s10103-018-2641-3.

LERNER, A. B., CASE, J. D., TAKAHASHI, Y., LEE, T. H., & MORI, W. 1958. Isolation of melatonin, the pineal gland factor that lightens melanocyte S1. Journal of the American Chemical Society, 80(10), 2587-2587.‏ https://doi.org/10.1021/ja01543a060

LUCACIO, O., GHEBAN, D., SORITAU, O., BACIUT, M., CAMPIAN, R. S., and BACIUTţ, G., 2015. Comparative assessment of bone regeneration by histometry and a histological scoring system. Revista Română de Medicină de Laborator Vol, 23(1). https://sciendo.com/article/10.1515/rrlm-2015-0009.

MA, Q., REITER, R. J., and CHEN, Y., 2020. Role of melatonin in controlling angiogenesis under physiological and pathological conditions. Angiogenesis, 23(2), 91–104.

https://doi.org/10.1007/s10456-019-09689-7.

MEI, Q., YU, J.-P., XU, J.-M., WEI, W., XIANG, L., and YUE, L., 2002. Melatonin reduces colon immunological injury in rats by regulating activity of macrophages. Acta Pharmacologica Sinica, 23(10), 882–886. https://pubmed.ncbi.nlm.nih.gov/12370092/.

PUGAZHENTHI, K., KAPOOR, M., CLARKSON, A. N., HALL, I., and APPLETON, I., 2008. Melatonin accelerates the process of wound repair in full‐thickness incisional wounds. Journal of Pineal Research, 44(4), 387–396.

https://doi.org/10.1111/j.1600-079X.2007.00541.x.

SALDUZ, A., DIKICI, F., KILICOGLU, Ö. I., BALCIı, H. I., AKGUL, T., KURKCU, M., KURTOGLU, C., and TOZUN, R., 2017. Effects of NSAIDs and hydroxyapatite coating on osseointegration: Biomechanical and histological study on rabbits. Journal of Orthopaedic Surgery, 25(1), 2309499016684410. https://doi.org/10.1177%2F2309499016684410.

SALIM, H. A., and MUNAWAH, B. S., 2020. Histopathological Effects of Histoacryl on Oral Secondary Wound Healing: A Comparative Experimental Study. Al-Rafidain Dental Journal, 13(3), 515–523.

http://dx.doi.org/10.33899/rden.2020.165388.

SHARAFATI-CHALESHTORI, R., SHIRZAD, H., RAFIEIAN-KOPAEI, M., and SOLTANI, A., 2017. Melatonin and human mitochondrial diseases. Journal of research in medical sciences: the official journal of Isfahan University of Medical Sciences, 22.‏https://dx.doi.org/10.4103%2F1735-1995.199092

SOYBIR, Gü., TOPUZLU, C., ODABAS, Ö., DOLAY, K., BILIR, A., and KOKSOY, F., 2003. The effects of melatonin on angiogenesis and wound healing. Surgery Today, 33(12), 896–901.

https://doi.org/10.1007/s00595-003-2621-3.

TAN, D.-X., MANCHESTER, L. C., ESTEBAN-ZUBERO, E., ZHOU, Z., and REITER, R. J., 2015. Melatonin as a potent and inducible endogenous antioxidant: synthesis and metabolism. Molecules, 20(10), 18886–18906.

https://doi.org/10.3390/molecules201018886.

TAQA, G. A., MUSTAFA, E. A., and Al-HALIEM, S. M., 2014. Evaluation of Anti-Bacterial and Efficacy of plant extract (Urtica urens) on Skin Wound Healing in Rabbit. International Journal of Enhanced Research in Science Technology & Engineering, 3(1), 64–70. http://www.erpublications.com/.

TAQA, G.A. Al-SHEIKH, H.A., and Al-ALLAF, L.I., 2021. Effects of Melatonin on Behavioural Activities in Acetaminophen-induced Autism in Rat. Journal of Applied Veterinary Sciences.

https://journals.ekb.eg/article_193738_0.html.

XU, Y., CHEN, X., QIAN, Y., TANG, H., SONG, J., QU, X., YUE, B., and YUAN, W., 2020. Melatonin‐Based and Biomimetic Scaffold as Muscle–ECM Implant for Guiding Myogenic Differentiation of Volumetric Muscle Loss. Advanced Functional Materials, 30(27), 2002378. https://doi.org/10.1002/adfm.202002378.

YEYEN, S., KARAKAS, D. O., BUDAK, E. T., and YILMAZ, I., 2013. The effects of different concentrations of epinephrine adjuvant to levobupivacaine on wound healing. Archives of Clinical and Experimental Surgery (ACES), 2(2), 92–96. http://dx.doi.org/10.5455/aces.20121002024412.

YULIIATI, K. T., RANANDA, I., and SOESILAWATI, P., 2020. Increasing macrophages in tooth extraction wound healing after induction of freeze-drying gel Aloe vera 90% on Cavia cobaya. Malaysian J Med Heal Sci, 16(4), 87–91.

https://medic.upm.edu.my/upload/dokumen/2020070611253117_MJMHS_0164.pdf