Characterization of Egyptian Isolates of Canine Distemper and Canine Parvoviruses

Document Type : Original Article

Authors

1 Veterinary Serum and Vaccine Research Institute, Agriculture Research Center, Abasia,Cairo, Egypt.

2 Central Laboratory for Evaluation of Veterinary Biologics, Agriculture Research Center, Abasia, Egypt.

Abstract

This study was aimed to investigate the incidence of CD and CP viruses in Cairo, Egypt. In a private Vet. Clinic in Cairo, five local breed puppies of about 3-5 months of age were supposed to be infected with the virus of canine distemper (CDV) showing fever, runny nose, salivation and coughing. Another 6 German Shepard and 4 Gryphon puppies of about 6-9 months of age were suffered from fever and bloody diarrhea suspected to be infected with canine parvovirus (CPV). All of these puppies had no history of vaccination. Trials of virus segregation in Vero and MDCK cell lines and usage of virus neutralization test (VNT) using specific anti-CD and anti-CP sera revealed that 3 out of 5 nasal swabs were positive to CD virus and 3 out of 10 fecal swabs were positive to CPV. These results were confirmed by Rt-PCR showing positive amplification with CD and CP, respectively. So, we could say that VNT and Rt-PCR are essential assays to identify CD and CP viruses and puppy vaccination should not be neglected to protect them against such diseases.

Keywords

Main Subjects

References

ANDREAS, BEINEKE; BAUMGÄRTNER, WOLFGANG; WOHLSEIN, PETER, 2015. Cross-species transmission of canine distemper virus—an update." One Health. 1: 49–59.
APPEL MJG, COOPER BJ, GREISEN H. AND CARMICHAEL LE., 1978. Status report: canine viral enteritis. J Am Vet Med Assoc; 173:1516–1518.
APPEL MJG, SCOTT FW. AND CARMICHAEL LE., 1979. Isolation and immunization studies of a canine parvo-like virus from dogs with hemorrhagic enteritis. Vet Res; 105:156–159.
BLACK JW, HOLSCHER MA, POWELL HS. AND BYERLY CS., 1979. Parvoviral enteritis and panleucopenia in dogs. J Med Small Anim. Clin; 74:47–50.
CARLETON HM., 1967. Histological technique 4th Ed., New York, London and Toronto, Oxford University. Press
 CARMICHAEL, L.E. AND JOUBERT, R.V.H., 1983. A modified live canine parvovirus vaccine 2, Immune response; Cornell Vet. 73: 13-29.
CHO HS, SONG JE, PARK YS. AND PARK NY., 2004. Diagnosis of the canine parvovirus in fecal samples by in situ hybridization. Int Vet J; 81:855–859.
DECARO N, ELIA G, CAMPOLO M, DESARIO C, LUCENTE MS. AND BELLACICCO AL., 2005. New approaches for molecular characterization of canine parvovirus type-2 strains. J Vet Med B ; 52:316–319.
DECARO N, ELIA G, MARTELLA V, CAMPOLO M, DESARIO C. AND  CAMERO M., 2006. Characterization of the canine parvovirus type 2 variants using minor groove binder probe technology. J Virol. Methods; 133:92.
DEEM, SHARON L.; SPELMAN, LUCY H.; YATES, REBECCA A.; MONTALI AND RICHARD J., 2000. Canine Distemper in Terrestrial Carnivores: A Review," Journal of Zoo and Wildlife Medicine. 31 (4):441–451. 
DEWEY, C.W., 2003. A Practical Guide to Canine and Feline Neurology. Iowa State Press 
GABRIELLA ELIA; NICOLA DECARO; VITO MARTELLA AND FRANCESCO CIRONE, 2006. Detection of canine distemper virus in dogs by real-time RT-PCR; Journal of Virological Methods 136 (1-2):171-6.
GREENE, CE; VANDEVELDE, M., 2012. "Chapter 3: Canine distemper". In Greene, Craig E. (ed.). Infectious diseases of the dog and cat (4th ed.). St. Louis, Mo.: Elsevier/Saunders. pp. 25–42
HAYES MA, RUSSELL RG, MUELLER RW AND LEWIS RJ., 1979. Myocarditis in young dogs associated with a parvovirus-like agent. Can Vet J; 20:126–132. 
HO-SEONG C, JONG K. AND NAM-YONG P., 2006. Detection of canine parvovirus in fecal samples using loop-mediated isothermal amplification. J Vet Diagn Investigation; 18:81–84. 
HIRAYAMA, K.; KANO, R.; KANANI, TH; TUCHIYA, K.; TSUYAMA, S.; NAKAMURA, Y.; SASAKI, Y. AND HASEGAWA, A., 2005. VP2gene of a canine parvovirus isolate from stool of a puppy, J. Vet. Med. Scib. 67: 139-143
HOANG M, WU H-Y, LIEN Y-X, CHIOU M-T, LIN C-N., 2019. A SimpleProbe® real-time PCR assay for differentiating the canine parvovirus type 2 genotype. J Clin Lab Anal. 2019;33: e22654
https://doi.org/10.1002/jcla.22654
IKEDA, YASUHIRO; NAKAMURA, KAZUYA; MIYAZAWA, TAKAYUKI; CHEN, MING-CHU; KUO, TZONG-FU; LIN, JAMES A; MIKAMI, TAKESHI; KAI, CHIEKO AND TAKAHASHI, EIJI.,  2001. "Seroprevalence of Canine Distemper Virus in Cats." Clin Vaccine Immunol. 8 (3):641–644. 
JIANCHANG WANG; JINFENG WANG; RUIWEN LI, LIBING LIU & WANZHE YUAN, 2017.  Rapid and sensitive detection of canine distemper virus by real-time reverse transcription recombinase polymerase amplification; BMC Veterinary Research volume 13, Article number: 241.
KUMAR M, CHIDRI S. AND  NANDI S., 2010. Molecular cloning and restriction endonuclease analysis of canine parvovirus DNA amplified by polymerase chain reaction. Glob Vet., 4(2):125–129. 
MEREDITH M CLANCY , KATHRYN C GAMBLE, DOMINIC A TRAVIS, 2013. Detection of canine distemper virus serum neutralizing antibodies in captive US phocids; Zoo Wildl Med., Mar;44(1):70-8.
MOHAMED Z. SAYED-AHMED; ZAHRAA ELBAZ; EMAD YOOUNIS AND MOHAMED H. KHODEIR , 2020. Canine parvovirus infection in dogs: Prevalence and associated risk factors in Egypt; World Vet. J. 10 (4): 571-577.
NANDI S, KUMAR M, ANBAZHAGAN R, CHIDRI S. AND CHAUHAN RS., 2007. A sensitive method to detect canine parvoviral DNA in the stool samples by polymerase chain reaction. Indian J Comp Microbiol Immunol Infect Dis., 27:56–57. 
NANDI S, CHIDRI S, KUMAR M. AND  CHAUHAN RS. 2010. Occurrence of canine parvovirus type 2c in the dogs with hemorrhagic enteritis in India. Res Vet Sci., 88:169–171.
PARRISH, C.R., CARMICHAEL, L.E. AND ANTCZAK, D.F., 1982. Antigenic relationships between canine parvovirus type 2, feline panleukopenia virus and mink enteritis virus using conventional antisera and monoclonal antibodies. Archives of Virology 72, 267–278
PEREIRA CA, MONEZI TA, MEHNERT DU, D’ANGELO M. AND DURIGON EL., 2000. Molecular characterization of canine parvovirus in Brazil by PCR. Vet Microbiol., 75:127–133.
RABIE ETMAN; MAHMOUD S. SAFWAT AND MOHAMED H. KHODEIR,2021. Molecular characterization of canine parvovirus-2 (CPV-2) in dogs in Egypt: Adv. Anim. Vet. Sci. 9 (6): 933-940
RAMADASS P. AND KHADHER TGA., 1982. Diagnosis of canine parvovirus infection by agar gel precipitation test and fluorescent antibody techniques. Cheriton., 11:323–326. 
ROFAIDA M. SOLIMAN; NABIL M. BAKER; MOHAMED Y. NASR AND MOHAMED H. KHODEIR, 2018. Clinical, virological and molecular characterization of canine parvovirus in dogs; EJPMR, 5 (4): 525-535
SAMBROOK, J.; FRITSCH, E.F. AND MANIATIS, T., 1989. Molecular cloning: A laboratory manual, 2nd ed.Cold Spring Harbor Lab., NY.
SCAGLIARINI A, DAL POZZO F, GALLINA L, VACCARI F. AND MORGANTI, L., 2007. TaqMan based real time PCR for the quantification of canine distemper virus. Vet Res Commun. 31: 261–263. pmid:17682890
SEKI F, ONO N, YAMAGUCHI R. AND YANAGI Y., 2003. Efficient isolation of wild strains of canine distemper virus in Vero cells expressing canine SLAM (CD150) and their adaptability to marmoset B95a cells. J Virol ; 77:9943–9950
SERAGELDIN SULTAN, NGUYEN THI LAN TOSH-IKIUEDA, RYOJIYAMAGUCHI, KEN MAEDA AND KAZUSHIGE K.A.I., 2009. Propagation of Asian isolates of canine distemper virus (CDV) in hamster cell lines; Acta Vet Scand., 51(1): 38.
SHISHIDO A.; K. YAMANOUCHI; M. HIKITA; T. SATO; A. FUKUDA AND F. KOBUNE, 1967. Development of a cell culture system susceptible to measles, canine distemper, and rinderpest viruses; Archiv für die gesamte Virus for schung September 1967, Volume 22, Issue 3–4, pp 364–380
TOMASZEWICZ BROWN A, MCALOOSE D, CALLE PP, AUER A, POSAUTZ A, SLAVINSKI S., BRENNAN R., WALZER, C.,. AND SEIMON, T. A., 2020. Development and validation of a portable, point-of-care canine distemper virus qPCR test. PLOS ONE 15(4): e0232044. https://doi.org/10.1371/journal.pone.0232044
UNIVERSITY OF WISCONSIN MADISON SHELTER MEDICINE, 2010. Canine distemper. https://www.uwsheltermedicine.com/library/guidebooks/canine-parvovirus/introduction-and-significance-in-a-shelter
WOODS CB, POLLOCK RVH. AND CARMICHAEL LE., 1980. Canine parvoviral enteritis. J Am Anim Hosp Assoc., 16:171–179
YONEDA, A.; TUCHIYA, K.; TAKASHIMA, Y.; ARAKAWA, T.; TSUJI, N.; HAYASHI, Y. AND MATSUMOTO, Y., 2008. Protection of mice from rabies by intranasal immunization with inactivated rabies virus. Exp. Anim., 57 (1): 1-9
YONG HWAN KIM; KYU-WOAN CHO; HWA YOUNG YOUN; AND HWA YOUNG YOUN, 2001. Detection of canine distemper virus (CDV) through one-step RT-PCR combined with nested PCR; Journal of Veterinary Science 2(1):59-63

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History

  • Receive Date: 05 July 2021
  • Revise Date: 14 August 2021
  • Accept Date: 18 August 2021

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