Khodeir, M., A.M., E., El-Shamandy, O., Y.A., S., A.T., E., M. Saber, S. (2021). Characterization of Egyptian Isolates of Canine Distemper and Canine Parvoviruses. Journal of Applied Veterinary Sciences, 6(4), 1-6. doi: 10.21608/javs.2021.84062.1088
M. H. Khodeir; Elbehwar A.M.; Omaima A.E. El-Shamandy; Soliman Y.A.; El-dakhly A.T.; Sahar M. Saber. "Characterization of Egyptian Isolates of Canine Distemper and Canine Parvoviruses". Journal of Applied Veterinary Sciences, 6, 4, 2021, 1-6. doi: 10.21608/javs.2021.84062.1088
Khodeir, M., A.M., E., El-Shamandy, O., Y.A., S., A.T., E., M. Saber, S. (2021). 'Characterization of Egyptian Isolates of Canine Distemper and Canine Parvoviruses', Journal of Applied Veterinary Sciences, 6(4), pp. 1-6. doi: 10.21608/javs.2021.84062.1088
Khodeir, M., A.M., E., El-Shamandy, O., Y.A., S., A.T., E., M. Saber, S. Characterization of Egyptian Isolates of Canine Distemper and Canine Parvoviruses. Journal of Applied Veterinary Sciences, 2021; 6(4): 1-6. doi: 10.21608/javs.2021.84062.1088
Characterization of Egyptian Isolates of Canine Distemper and Canine Parvoviruses
1Veterinary Serum and Vaccine Research Institute, Agriculture Research Center, Abasia,Cairo, Egypt.
2Central Laboratory for Evaluation of Veterinary Biologics, Agriculture Research Center, Abasia, Egypt.
Receive Date: 05 July 2021,
Revise Date: 14 August 2021,
Accept Date: 18 August 2021
Abstract
This study was aimed to investigate the incidence of CD and CP viruses in Cairo, Egypt. In a private Vet. Clinic in Cairo, five local breed puppies of about 3-5 months of age were supposed to be infected with the virus of canine distemper (CDV) showing fever, runny nose, salivation and coughing. Another 6 German Shepard and 4 Gryphon puppies of about 6-9 months of age were suffered from fever and bloody diarrhea suspected to be infected with canine parvovirus (CPV). All of these puppies had no history of vaccination. Trials of virus segregation in Vero and MDCK cell lines and usage of virus neutralization test (VNT) using specific anti-CD and anti-CP sera revealed that 3 out of 5 nasal swabs were positive to CD virus and 3 out of 10 fecal swabs were positive to CPV. These results were confirmed by Rt-PCR showing positive amplification with CD and CP, respectively. So, we could say that VNT and Rt-PCR are essential assays to identify CD and CP viruses and puppy vaccination should not be neglected to protect them against such diseases.
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