Rasheed, T., Jwher, D. (2021). Isolation, identification and sensitivity of Mannheimia haemolytica from Ewes udder with clinical mastitis. Journal of Applied Veterinary Sciences, 6(3), 25-30. doi: 10.21608/javs.2021.76049.1079
T. J. Rasheed; Dh. M. Jwher. "Isolation, identification and sensitivity of Mannheimia haemolytica from Ewes udder with clinical mastitis". Journal of Applied Veterinary Sciences, 6, 3, 2021, 25-30. doi: 10.21608/javs.2021.76049.1079
Rasheed, T., Jwher, D. (2021). 'Isolation, identification and sensitivity of Mannheimia haemolytica from Ewes udder with clinical mastitis', Journal of Applied Veterinary Sciences, 6(3), pp. 25-30. doi: 10.21608/javs.2021.76049.1079
Rasheed, T., Jwher, D. Isolation, identification and sensitivity of Mannheimia haemolytica from Ewes udder with clinical mastitis. Journal of Applied Veterinary Sciences, 2021; 6(3): 25-30. doi: 10.21608/javs.2021.76049.1079
Isolation, identification and sensitivity of Mannheimia haemolytica from Ewes udder with clinical mastitis
Department of Veterinary Public Health, College of Veterinary Medicine, University of Mosul, Iraq.
Receive Date: 11 May 2021,
Revise Date: 13 June 2021,
Accept Date: 16 June 2021
Abstract
The current study aims to isolate and identify M. haemolytica from ewes with clinical mastitis using conventional techniques with comparative study to their resistance to several types of antibiotics for the isolated bacteria from udder. The study was carried out in different regions of Nineveh governorate. Two Hundred sixty-six samples (133 milk samples and 133 udder skin swabs) were collected During December 2020, after the clinical examinations of the flocks. The samples were put in test tubes containing Brain Heart Infusion (BHI) Broth; then transferred into the Veterinary Public Health Laboratory, College of Veterinary Medicine, University of Mosul for different bacteriological, biochemical examination and sensitivity tests. The results showed that there is a matching between bacterial isolation ratios of milk and udder skin swabs of sheep affected by clinical mastitis at 51.12%. The results also showed a difference in the sensitivity test between M. haemolytica isolated from milk and skin swabs. It can be concluded that, the improper and indiscriminate use of veterinary medicines is one of the most important causes for the increase in bacterial resistance to antibiotics in treated cases without consulting a veterinarian.
AMIR, H.A., 2013. Mastitis in housed dairy buffaloes: incidence, etiology, clinical finding, antimicrobial sensitivity and different medical treatment against E.coli mastitis. Life Sci. J.;10 (1): pp 532-538.
AHMED, S. AND JWHER, D., 2019. Detection Of Streptomycin Residues In Sheep Carcasses In Duhok Province, Kurdistan Region, Iraq. Journal of Applied Veterinary Sciences, 4(2), pp.41–44.
ARSENAULT, J., DUBREUIL, P., HIGGINS, R., AND BÉLANGER, D., 2008. Risk factors and impacts of clinical and subclinical mastitis in commercial meat-producing sheep flocks in Quebec, Canada. Preventive Veterinary Medicine, 87(4): 373-393. doi.org/10.1016/j.prevetmed.2008.05.006
BAUER, A. W., KIRBTY, W. A. M., SHERRIES, J. S., AND TURK, M., 1996. Antibiotics susceptibility testing by a standardized single disk method. Am. J. Clin. Patho., 45(1):495-499.
BERGONIER, D., BERTHELOT, X., 2003. New advances in epizootiology and control of ewe mastitis. Livest. Prod. Sci. Elsevier BV;79 (1):1-16. doi.org/10.1016/s0301-6226(02)00145-8.
BERGONIER, D., DE CREMOUX, R., RUPP, R., LAGRIFFOUL, G., BERTHELOT, X., 2003. Mastitis of small dairy ruminants. Vet. Res. 34(5):689-716.
BETERMIER, M., POQUET, I., ALAZARD, R., AND CHANDLER, M., 1993. Involvement of Escherichia coli FIS protein in maintenance of bacteriophage Mu lysogeny by the repressor: control of early transcription and inhibition of transposition. J. Bacteriol. 175:3798–3811. doi.org/10.1128/jb.175.12.3798-3811.1993.
BOJESEN, A.M., LARSEN, J., PEDERSEN, A.G., MÖRNER, T., MATTSON, R., AND BISGAARD, M., 2007. Identification of a novel Mannheimia granulomatis lineage from the lesions in roe deer (Capreolus capreolus). J. Wildlife. Dis., 43(1): 345-352.
BORKAR, SG, 2017. Biochemical Tests Used in Identification of Bacteria. Laboratory Techniques in Plant Bacteriology, pp.93-108. doi.org/10.1201/9781315206882-17.
BOURABAH, A., AYAD, A., BOUKRAA, L., HAMMOUDI, S., BENBAREK, H., 2013. Prevalence and etiology of subclinical mastitis in goats of the Tiaret region, Algeria. Glob. Vet., 11(1):604-608.
BURRIEL A. R., 1997. Isolation of Pasteurella haemolytica from grass, drinking water and straw bedding used by sheep. Curr. Microbiol., 35(1):316-318.
BURROWS, G. E., 1980. Therapeutic consideration in the use of antibacterials. Bov. Prac. (15): 99-102.
CONTRERAS, G.A., AND RODRIGUEZ, J.M., 2011. Mastitis: comparative etiology and epidemiology. Journal of Mammary Gland Biology and Neoplasia, 16(4): 339-356.
Ultrasonography, Histopathological Udder Alterations and Bacteriological Investigations For Diagnosis Of Mastitic Goats. Journal of Applied Veterinary Sciences .5(2):2020, Pp: 77-86. https://dx.doi.org/10.21608/javs.2020.85593
FTHENAKIS, G.C., ARSENOS, G., BROZOS, C., FRAGKOU, I.A., GIADINIS, N.D., GIANNENAS, I., 2012. Health management of ewes during pregnancy. Anim. Reprod. Sci. Elsevier BV; 130(4):198212.doi.org/10.1016/j.anireprosci.2012.01.016.
GARROD, L. P., LANBERT, HB, OGRADY, F., WATER WORTH, P . M., 1981. Antibiotic and chemotherapy. 5th.ed. Churchill Living stone, Edinburgh, pp; 262-271.
HEIN, WR, 2000. Breeding for Disease Resistance in Farm Animals, 2nd Edition. Eds. Axford, R.F.E., Bishop S.C., Nicholas, F.W. and Owen, J.B. Wallingford, Oxon, CABI Publishing, 418 pp. £75 (hard). The Veterinary Journal. Elsevier BV; 161(2):109. doi.org/10.1053/tvjl.2000.0510
KOTB, E. & GAFER, J., 2020. Molecular Detection Of Toxins And Disinfectant Resistance Genes Among Staphylococcus Aureus Isolated From Dairy Cattle In Egypt. Journal of Applied Veterinary Sciences, 5(1):35–45. https://dx.doi.org/10.21608/javs.2020.75411
LARSGARD, A., AND VAABENOE, A., 1993. Genetic and environmental causes of variation in mastitis in sheep. Small Ruminant Res., 12(3): 339-347. Doi: 10.1016/0921-4488(93)90069-T.
MACFADDIN, GF, 2000. Biochemical test for identification of medical bacteria. 3ed. William and Wilkins, USA.
MICHAEL, GB, KADLEC, K., SWEENEY, MT, 2012. ICEPmu1, an integrative conjugative element (ICE) of Pasteurella multocida: analysis of the regions that comprise 12 antimicrobial resistance genes. J. Antimicrob. Chemother. 67(1):84-90. doi.org/10.1093/jac/dkr406
OMALEKI, L., BARBER, SR, ALLEN, JL, BROWNING, GF, 2010. Mannheimia species associated with ovine mastitis. J. Clin. Microbiol. 48(1):3419-3422. doi.org/10.1128/jcm.01145-10
ONNASCH, H., HEALY, A.M., BROPHY, P., KINSELLA, A., DOHERTY, ML, 2002. A study of mastitis in Irish sheep. Res. Vet. Sci. 72(1):42-44. doi.org/10.1016/s0034-5288(02)90120-7
PEELER, E.J., GREEN, M.J., FITZPATRICK, J.L., GREEN, L.E., 2002. Study of clinical mastitis in British dairy herds with bulk milk somatic cell counts less than 150,000 cells/ml. Vet. Record. Wiley; 151(6):170-176. doi.org/10.1136/vr.151.6.170
PORTIS, E., LINDEMAN, C., JOHANSEN, L., 2012. A ten-year (2000-2009) study of antimicrobial susceptibility of bacteria that cause bovine respiratory disease complex Mannheimia haemolytica, Pasteurella multocida, and Histophilus somni - in the United States and Canada. J Vet Diagn.Invest., 24(1):932-944.
QUINN, PJ, MARKEY, BK, LEONARD, FC, HARTIGAN, P., FANNING, S. AND PATRICK, ESF, 2011. Veterinary Microbiology and Microbial Disease. John Willy & Son Ltd, UK.
RADOSTITS, O., GAY, C., BLOOD, D., AND HINCHCLIFF, K., 2000. Veterinary Medicine. A textbook of the diseases of cattle, sheep, pigs, goats and horses. WB Saunders Company Ltd. Edinburgh, London, New York. Oxford, Philadelphia, St. Louis, Sydney, Toronto.
SAHAY, S., NATESAN, K., PRAJAPATI, A., KALLESHMURTHY, T., SHOME, BR, RAHMAN, H., AND SHOME, R., 2020. Prevalence and antibiotic susceptibility of Mannheimia haemolytica and Pasteurella multocida isolated from ovine respiratory infection: A study from Karnataka, southern India. Veterinary World 13(9):1947-1954.
SANCHIS, R.; AND ABADIE, G., 1992. Antimicrobial susceptibility of Pasteurella haemolytica and Pasteurella trehalosi. Recucilde medicine. Veterinair. Vol. 168(10):807-813.
SURETTE, M.G., BUCH, S.J., AND CHACONAS. G., 1987. Transpososomes: stable protein-DNA complexes involved in the in vitro transposition of bacteriophage Mu DNA. Cell 49(1):254-262.
YOUSIF, SA, JWHER, DM 2021. Detection of multiple presences of antibiotic residues in slaughtered sheep at Duhok abattoir, Iraq. Iraqi J. Vet. Sci. 35(1):49-55. doi.org/10.33899/ijvs.2019.126259.1276
ZANINELLI, M., ROSSI, L., COSTA, A., TANGORRA, F.M., AGAZZI, A., SAVOINI, G., 2015. Signal spectral analysis to characterize gland milk electrical conductivity in dairy goats. Ital. J. Anim. Sci. 14(3):362-367. /doi.org/10.4081/ijas.2015.3518
ZECCHINON, L., FETT, T., AND DESMECHT, D., 2005. How Mannheimia haemolytica defeats host defense through a kiss of death mechanism. Vet. Res., 36(1):133-156. doi.org/10.1051/vetres:2004065.
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