A. E. El-Shamandy, O., Abd El-Aziz, H. (2020). Efficacy Of Lyophilized Inactivated Rabies Vaccine. Journal of Applied Veterinary Sciences, 5(4), 68-72. doi: 10.21608/javs.2020.120348
Omaima A. E. El-Shamandy; Heba M.G Abd El-Aziz. "Efficacy Of Lyophilized Inactivated Rabies Vaccine". Journal of Applied Veterinary Sciences, 5, 4, 2020, 68-72. doi: 10.21608/javs.2020.120348
A. E. El-Shamandy, O., Abd El-Aziz, H. (2020). 'Efficacy Of Lyophilized Inactivated Rabies Vaccine', Journal of Applied Veterinary Sciences, 5(4), pp. 68-72. doi: 10.21608/javs.2020.120348
A. E. El-Shamandy, O., Abd El-Aziz, H. Efficacy Of Lyophilized Inactivated Rabies Vaccine. Journal of Applied Veterinary Sciences, 2020; 5(4): 68-72. doi: 10.21608/javs.2020.120348
Efficacy Of Lyophilized Inactivated Rabies Vaccine
1Veterinary Serum and Vaccine Research Institute, Abassia, Cairo, Agriculture Research Center (ARC), Egypt.
2Central Laboratory for Evaluation of Veterinary Biologics, Abassia, Agriculture Research Center (ARC), Egypt.
Receive Date: 19 September 2020,
Revise Date: 13 October 2020,
Accept Date: 21 October 2020
Abstract
An interesting finding that emerged from the study was the difference in potency between inactivated rabies vaccines prepared in liquid and lyophilized forms. Inactivated rabies vaccine prepared from BHK21 infected cell culture in the two forms revealed that its immunogenicity was completely preserved on lyophilization. The two vaccine formulae were found to be free from foreign contaminants, safe in both inoculated mice and puppies and potent inducing high levels of specific rabies antibodies in vaccinated puppies. Such antibodies were increased gradually from the first-week post-vaccination to the same extent in the blood of immunized puppies with the lyophilized vaccine as in that of puppies immunized with liquid vaccine. These results confirmed the claim that inactivated rabies vaccines could be lyophilized with retention of potency. Considering the possible value of a stable, freeze-dried rabies vaccine that could be stored and transported with ease, it was considered desirable to confirm the above findings.
AL-BEHWAR, A.M., 2009. Studies on prophylactic and emergency vaccination of farm animals against rabies, Ph. D. Thesis (Infectious diseases) Fac. Vet. Med. Cairo Univ.
AMANI, A. SALEH; EL-GALLAD, S.B.; KHODEIR, M.H. AND AZAB, A.M., 2002. Trivalent inactivated vaccine for dogs (Rabies, Canine Distemper and Canine Parvo), J. Egypt Vet. Med. Ass. 62, No.2: 209-222.
ATTYAT M. KOTB, 1994. Studies on preparation of canine parvo vaccine, Ph. D. Thesis (Microbiology) Fac. Vet. Med. Cairo Univ.
BASS, E.P.; GILL, M.A., AND BECKENHAUR, W.H., 1982. Development of a modified live canine origin parvovirus vaccine JAVMA, 181 (9): 909-913.
CHARLES. E.RUPPRECHT, STANLY.A.PLOTKIN 2013. Scientific Basis of the Disease and Its Management. Elsevier, Science Direct, sixth edition2013.
DEBORAH J.BRIGGS, THIRUMENI NAGARAJAN, CHARLES E.RUPPRECHT, 2013. Scientific Basis of the Disease and Its Management. Elsevier, Science Direct, third edition chapter13, 2013.
EDRIES, S. M., 1994. Production of inactivated tissue culture rabies vaccine,Ph. D. Thesis, Fac. Vet. Med. Virology, Cairo Univ
EDRIES, S.M.; AMANI, A. SALEH; ALBEHWAR, A.M. AND HEMMET, S. E. EL-SHAMY,2017. Improvement of inactivated rabies vaccine using montanid pet gel-aBenha J. Applied Sci. (BJAS) Vol (2) Issue (1) Oct. (2017), 145-149
EIAKA, A.M.E., 2017. Virological and immunological studies on the effect of bee venom on rabies virus, Ph. D. Thesis (Virology) Fac. Vet. Med. Benha Univ.
EL-KANAWATI, Z. R.; IKRAM, A. KARIM; AFAF, AMIN; AND EL-EBEEDY, A. A., 2000. Occurrence of rabies in Egypt during 1997-1999, J. Egyp. Vet. Med. Ass., 60: 47-54.
FAO., 1994. Quality control of rinderpest cell culture vaccine, Standard Operating Procedures, FAO report No.118
GIRARD, H.C.; BAYRAMOGLU, O.; EROL., N. AND BURGUT, A., 1977. Inactivation of (O1) FMD virus by binary ethylenimine (BEI), Bull. Off. Int. Epiz. 87(3-4): 201-217.
KHODIER, M. H., 1999. Studies on vaccination of farm animals (cattle, horse, sheep) in addition to dogs and cats with inactivated tissue culture rabies vaccine, Beni-Suif Vet. Med. J.;9 (3-A): 111-120.
KHODEIR, M. H. AND DAOUD, A. M., 2008. Preparation of antirabies hyperimmune serum for emergency Immunization of farm animals. 4th Int. Sci. Conf. NRC, 1-9 .
LARGHI, O.P. AND NEBEL, A.E., 1980. Rabies virus inactivation by Binary Ethyleneimine: New method for inactivated vaccine production, J.Clic.Microbiol.2: 120-122
NIGG, A. J. AND WALKER, P. L., 2009. Overview: prevention and treatment of rabies, Pharmacotherapy 2009 Oct.; 29 (10): 1182-1185.
REED, L.J. AND MUENCH, H., 1938. A simple method for estimating fifty percent (50%) endpoints, Amer. J. Hyg., 27: 493-497.
RUPPORECHT, C. E.;BRIGGS, D. AND BROWN, C. M., 2010. Use of a reduced (4 doses) vaccine schedule for post-exposureProphylaxis to prevent human rabies recommendations of the AdvisoryCommittee on immunization practices. MMWR Recomm. Rep. 59:1-9.
SHAO-ZHI, Z.; HUAN, Q.; ZHEN, W.; JU-LI, F.; QIAN, Z.; GUANG-MING, C.; RUI, L.; SHAN, F. AND JIE, S., 2010. Preliminary study on the freeze-drying of human bone marrow-derived mesenchymal stem cells, J. Zhejiang Univ. Sci. Bv., 11 (11).
SINGH, K.V.; OSMAN, O.A.; THANAA, I.BAZ AND IVON, EL-CICY, 1967. Colostral transfer of rinderpest neutralizing antibodies offspring of vaccinated dams,Can. J. Comp. Med. Vet. Sci., 31: 295-298.
VEERARAGHAVAN, N. AND SUBRAHMANYAN, T.P., 1961. Antigenic Value of Lyophilized Phenolized Antirabies Vaccine, Bull. Org. Mond. Santg Bull. Wid Hlth.Org.1961, 25, 115-117.
WANG, J. AND ZHANG, H., 2007. The influence of one-step −80 °C cryopreservation on the osteogenesis differentiation ability of human bone marrow-derived mesenchymal stem cells, Guangdong Med., 28(3):365–367.
WHO 1973. Expert committee on biological standardization; requirements of rabies vaccine for human use, Tech. Rep. Ser., 658: 54-130.
WHO (2018): Rabies vaccines and immunoglobulins, WHO position in April 2018.
YONEDA, A.; TUCHIYA, K.; TAKASHIMA, Y.; ARAKAWA, T.; TSUJI, N.; HAYASHI, Y. AND MATSUMOTO, Y., 2008. Protection of mice from rabies by intranasal immunization with the inactivated rabies virus. Exp. Anim., 57 (1): 1-9.
ZEINAB, T. S. SALAMA; NAGLAA, I. ALI; GUIRGUIS, W.I. AND KHODEIR, M.H., 2003. Modification of the inactivated trivalent vaccine (Rabies; canine distemper and canine parvo) used for dogs to improve its immunogenicity, J. Egypt Vet. Med. Ass. 63, No.2: 137-146.
ZHOU, X.L.; ZHU, H.; ZHANG, S.Z.; ZHU, F.M.; CHEN, G.M. AND YAN, L.X., 2007. Freeze-drying of human platelets: influence of saccharide, freezing rate and cell concentration, Cryo Letters., 28(3):187–196.